水飞蓟复方制剂对急性酒精性肝损伤的保护作用研究

目的探究水飞蓟复方制剂对急性酒精性肝损伤小鼠的保护作用。方法将60只雄性KM小鼠随机分为空白对照组,模型组,水飞蓟复方制剂低、中、高剂量组,共5组,每组12只。空白对照组和模型组灌胃相同体积的无菌水,其余各组灌胃对应的水飞蓟复方制剂溶液,在灌胃的第28 d,除空白对照组外,其余组小鼠每隔12 h灌胃50%酒精,共计3次,诱导小鼠急性酒精性肝损伤模型。采用苏木精-伊红(hematoxylin eosin,HE)染色观察小鼠肝脏病理学变化;测定小鼠肝脏中丙二醛(malondialdehyde,MDA)、谷胱甘肽(glutathione,GSH)、甘油三酯(triglyceride,TG)的含量和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、总超氧化物歧化酶(total superoxide dismutase,T-SOD)、过氧化氢酶(catalase,CAT)的活性,测定小鼠血清中谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)的活性以及白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的水平。结果HE染色显示水飞蓟复方制剂组中的肝细胞水肿、气球样变现象得到改善;与模型组相比,在水飞蓟复方制剂高剂量组中,GSH含量显著升高(P<0.05),在其中剂量组中,T-SOD、CAT活性显著升高(P<0.05),MDA含量显著降低(P<0.05);利用生化分析仪测定水飞蓟复方制剂的转氨酶活性,发现在其中、高剂量组中的ALT、AST活性显著降低(P<0.01);酶联免疫吸附(enzyme linked immunosorbent assay,ELISA)法测定各组炎症因子水平,显示水飞蓟复方制剂低剂量组中的IL-1β水平和中、低剂量组中TNF-α水平显著降低(P<0.01)。结论水飞蓟复方制剂通过提高肝脏的抗氧化能力、降低血液中转氨酶活性、抑制促炎细胞因子的产生,从而在急性酒精性肝损伤中发挥一定的保护作用,为相关保健食品的研发应用提供一定价值的数据支持。

重组人生长激素在多囊卵巢综合征不孕患者中的应用

重组人生长激素在辅助生殖领域中被广泛应用于不孕症人群,其中就包括多囊卵巢综合征(PCOS)患者。rhGH通过基因重组技术产生,其理化性质与人体的生长激素完全相同。针对PCOS不孕患者,外源性补充rhGH可发挥纠正生长激素分泌不足、改善卵泡发育障碍、改善子宫内膜容受性及调节脂质代谢紊乱等作用,最终可能有利于改善PCOS患者的妊娠结局。但对于rhGH的适用人群及使用方法尚无统一标准。该文就rhGH在PCOS患者中的作用机制及具体应用作一综述。

绿色化工环保技术与环境治理的关系

随着社会和经济的快速发展,我国化工企业对于环境的污染问题也越来越严重。为了我国的可持续发展,需要实行绿色化工环保技术,加强环境治理,在传统化工企业能够良好发展的前提下,采取节能减排的方式,优化更新技术设备,使化工生产的过程中具有节约性和环保性,通过相关技术科学处理化学生产中的废料,避免污染周围环境。

化工给排水系统存在的不足与优化

随着我国现代经济社会的稳步建设,现代化工行业也迎来了发展的黄金周期,但对应的化工安全隐患现象却越发频繁。化工行业作为推动我国现代经济社会稳定建设的核心部分之一,区别于其他行业最明显的特征就在于,化工行业的发展建设包含了多种高危化学材料以及设备,稍有不慎就会造成巨大的化工安全隐患。而化工给排水系统作为现代化工行业发展的关键性保障,更是有效抑制化工火灾安全隐患的重要途径。对此,基于化工给排水系统的结构,分析当前化工给排水系统存在的不足,重点阐述如何解决化工给排水系统运行漏洞。

Dynamic Observation of Cartilage Damage and Serum IL-1β, TNF-α, IL-6 and IL-17 in Post-traumatic Rat Osteoarthritis Model

The pathogenesis of animal osteoarthritis(OA)is not clear.The aim of this study was to investigate the dynamic changes of cartilage lesions and serum IL-1β,TNF-α,IL-6 and IL-17 inflammatory factors in a post-traumatic osteoarthritis model in rats,and to explore their values in monitoring the progression of osteoarthritis.Forty male SD rats were randomly divided into the control group(n=20)and the model group(n=20).The OA model was established by anterior cruciate ligament transection combined with partial medial meniscus resection(ACLT+PMMx)in the model group,and sham operation was performed in the control group.On the 0th,10th,20th and 30th days after modeling,the degree of joint swelling and the number of white blood cells in the peripheral blood were evaluated,and tibia samples were taken for macroscopic observation score and pathological observation.The serum levels ofIL-1β,TNF-α,IL-6 and IL-17 concentration were detected by enzyme-linked immunosorbent assay(ELISA).The results showed that the cartilage damage in the tibial surface and pathological observations of rats in the model group gradually increased with time,the eye scores increased and increased significantly on the 20th and 30th days(P<0.01),and the joints of the rats were swollen.And the swelling was extremely significant on the 30th day(P<0.01).Compared with the control group,the number of white blood cells in the peripheral blood of the model group gradually increased,and the increase was extremely significant on the 20th and 30th days(P<0.01).The serum IL-17 concentration in the model group tended to increase,and the increase was significant on the 20th day(P<0.05)and extremely significant on the 30th day(P<0.01);the serum TNF-αand IL-1βconcentrations in the model group showed an increasing trend,and the increase was extremely significant on the 10th,20th and 30th days(P<0.01);IL-6 concentration in the serum of the model group showed an increasing trend,and it increased significantly on the 30th day(P<0.05).The results showed that in the rat model of traumatic OA,the degree of joint damage gradually increased,and the expression of blood inflammatory indexes IL-1β,TNF-α,IL-17 and IL-6 increased.The research provided a basis for prompting and monitoring the timing of OA treatment and the development of the disease in animal clinical.

环境应急水池管理研究

根据我国对于环境应急水池的规定和相关行业公司的管理方法,在生产过程中产生的废弃物如果对周围环境水质造成破坏或发生其他应急情况,则必须修建相应的环境应急水池,环境应急水池的设计和修建应该严格按照国家相关规定执行。基于此,提出了对于环境保护部门的环境应急水池类型结构和管理要求,使我国环境应急水池的建设和监管工作能够进一步完善。