Aim: To evaluate the effect of methoxychlor on the antioxidant system of goat epididymal sperm. Methods:Epididymis of adult goat was obtained from local slaughter houses and sperm were collected by chopping the epidid...Aim: To evaluate the effect of methoxychlor on the antioxidant system of goat epididymal sperm. Methods:Epididymis of adult goat was obtained from local slaughter houses and sperm were collected by chopping the epididymisin modified Ringer's phosphate solution (RPS). After several washings, the sperm samples were dispersed in RPS andincubated with methoxychlor (1μmol/L, 10μmol/L and 100μmol/L) and methoxychlor + vitamin C (100μmol/Leach) for 3 h at 32℃. After incubation, the sperm motility and viability were assessed. An aliquot of sperm samplewas homogenized, centrifuged and used for the assay of superoxide dismutase, glutathione peroxidase, glutathione re-ductase and lipid peroxidation. Results; In methoxychlor-incubated sperm and in sperm co-incubated with methoxy-chlor and vitamin C, the sperm motility and viability showed no significant changes as compared to the correspondingcontrols. In methoxychlor-incubated sperm the activity of superoxide dismutase, glutathione reductase and glutathioneperoxidase were decreased while lipid peroxidation was increased in a dose-dependent manner. Co-incubation of spermwith methoxychlor and vitamin C showed no changes in the activity of superoxide dismutase, glutathione reductase andglutathione peroxidase and in the level of lipid peroxidation. Conclusion; Methoxychlor induced oxidative stress inepididymal sperm of goats by decreasing the levels of antioxidant enzymes. Co-incubation of sperm with methoxychlorand vitamin C, a natural antioxidant, reversed the effect of methoxychlor. (Asian J Androl 2001 Dec; 3; 285 - 288)展开更多
文摘Aim: To evaluate the effect of methoxychlor on the antioxidant system of goat epididymal sperm. Methods:Epididymis of adult goat was obtained from local slaughter houses and sperm were collected by chopping the epididymisin modified Ringer's phosphate solution (RPS). After several washings, the sperm samples were dispersed in RPS andincubated with methoxychlor (1μmol/L, 10μmol/L and 100μmol/L) and methoxychlor + vitamin C (100μmol/Leach) for 3 h at 32℃. After incubation, the sperm motility and viability were assessed. An aliquot of sperm samplewas homogenized, centrifuged and used for the assay of superoxide dismutase, glutathione peroxidase, glutathione re-ductase and lipid peroxidation. Results; In methoxychlor-incubated sperm and in sperm co-incubated with methoxy-chlor and vitamin C, the sperm motility and viability showed no significant changes as compared to the correspondingcontrols. In methoxychlor-incubated sperm the activity of superoxide dismutase, glutathione reductase and glutathioneperoxidase were decreased while lipid peroxidation was increased in a dose-dependent manner. Co-incubation of spermwith methoxychlor and vitamin C showed no changes in the activity of superoxide dismutase, glutathione reductase andglutathione peroxidase and in the level of lipid peroxidation. Conclusion; Methoxychlor induced oxidative stress inepididymal sperm of goats by decreasing the levels of antioxidant enzymes. Co-incubation of sperm with methoxychlorand vitamin C, a natural antioxidant, reversed the effect of methoxychlor. (Asian J Androl 2001 Dec; 3; 285 - 288)
