A nanocomposite that incorporates cobalt oxide(Co3O4) and nanodiamond(ND) can present both high magnetism(Co3O4) and high hardness(ND). ND particles have potential applications in a variety of fields such as p...A nanocomposite that incorporates cobalt oxide(Co3O4) and nanodiamond(ND) can present both high magnetism(Co3O4) and high hardness(ND). ND particles have potential applications in a variety of fields such as protein immobilization, biosensors, therapeutic molecule delivery and bio-imaging. However,limited information is available in literature on the in-situ synthesis of biocompatible magnetic materials and also on their potential biotoxicity as a result of their entry into environmental compartments and subsequent interaction with biota. In this work, a new kind of bio-compatible magnetic material –carboxylated nanodiamond(c ND) and Co3O4 was synthesized to obtain the c ND-Co3O4 nanocomposite.The synthesis procedure involved in-situ and chemical reduction of cobalt chloride(CoCl(26)H2O) and sodium borohydrate(NaBH4). The synthesized cND-Co3O4 nanocomposite was characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy and X-ray photoelectron spectroscopy. The cyto-genotoxicity of the synthesized nanocomposite material was studied by using onion(Allium cepa L.) as a test model with concentrations of 5, 10 and 20 μg/ml. The study was also extended to cND and Co3O4 materials for comparison purpose. Co3O4 and cND exhibited their contrasting effects on mitosis and other cyto-genotoxic indices studied herein. This work provided fundamental data on the synthesis and the bio-toxicity of the c ND-Co3O4 nanocomposite, which, in turn, can help to expand their multidisciplinary applications.展开更多
基金the financial support from Foundation for the Science and Technology(FCT,Portugal)the funding through the post-doctoral grant SFRH/BPD/100003/2014(LSS)+3 种基金the 2013 FCT Investigator programme(MKS).NAA(SFRH/BPD/84671/2012)EP gratefully acknowledge the partial financial supports received from FCT(Government of Portugal)the Aveiro University Research Institute/CESAM(UID/AMB/50017/2013)“COMPETE”through Project no.FCOMP-01-0124-FEDER-02800(FCT PTDC/AGR-PRO/4091/2012)
文摘A nanocomposite that incorporates cobalt oxide(Co3O4) and nanodiamond(ND) can present both high magnetism(Co3O4) and high hardness(ND). ND particles have potential applications in a variety of fields such as protein immobilization, biosensors, therapeutic molecule delivery and bio-imaging. However,limited information is available in literature on the in-situ synthesis of biocompatible magnetic materials and also on their potential biotoxicity as a result of their entry into environmental compartments and subsequent interaction with biota. In this work, a new kind of bio-compatible magnetic material –carboxylated nanodiamond(c ND) and Co3O4 was synthesized to obtain the c ND-Co3O4 nanocomposite.The synthesis procedure involved in-situ and chemical reduction of cobalt chloride(CoCl(26)H2O) and sodium borohydrate(NaBH4). The synthesized cND-Co3O4 nanocomposite was characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy and X-ray photoelectron spectroscopy. The cyto-genotoxicity of the synthesized nanocomposite material was studied by using onion(Allium cepa L.) as a test model with concentrations of 5, 10 and 20 μg/ml. The study was also extended to cND and Co3O4 materials for comparison purpose. Co3O4 and cND exhibited their contrasting effects on mitosis and other cyto-genotoxic indices studied herein. This work provided fundamental data on the synthesis and the bio-toxicity of the c ND-Co3O4 nanocomposite, which, in turn, can help to expand their multidisciplinary applications.