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高频率产生芸苔属非整倍体和纯合植株及基因组原位杂交分析 被引量:10
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作者 李再云 刘焰 +3 位作者 m.ceccarelli S.Minelli A.Contento P.G.Cionini 《中国科学(C辑)》 CSCD 北大核心 2002年第3期218-224,T001,共8页
进行了芥菜型油菜(Brassica juncea(L.)Czern.& Coss.)和埃塞俄比亚芥(B.carinata A.Braun)与新油料植物资源诸葛菜(Orychophrogmus violacus(L.)O.E.Schulz)之间的属间杂交,并对产生的后代植株进行了基因组原位杂交分析... 进行了芥菜型油菜(Brassica juncea(L.)Czern.& Coss.)和埃塞俄比亚芥(B.carinata A.Braun)与新油料植物资源诸葛菜(Orychophrogmus violacus(L.)O.E.Schulz)之间的属间杂交,并对产生的后代植株进行了基因组原位杂交分析.结果表明,芥菜型油菜与诸葛菜的杂交后代由3类混倍体组成,第一类的体细胞和花粉母细胞(PMC)主要含有芥菜型油菜的36条染色体和附加的诸葛菜染色体(2n=36~44);第二类(2n=30~36)主要产生具有芥菜型油菜染色体的PMC(2n=36)和1~4对芥菜型油菜染色体被诸葛菜染色体所代换的代换型PMC(2n=36);第三类(2n=30~36)植株的PMC则只具有芥菜型油菜染色体(2n=36).而埃塞俄比亚芥与诸葛菜的杂交后代由两类混倍体(2n=29~34)组成,第一类的绝大多数PMC的染色体数目(2n=34)及行为与母本埃塞俄比亚芥植株的一样,只是部分PMC包含1~3对诸葛菜染色体,而第二类的PMC只具有埃塞俄比亚芥的34条染色体.从以上杂种的后代中获得了芸苔属亲本种的附加系、代换系、亚倍体和纯合植株.这些结果为在尝苔属与诸葛菜属间杂交中可能发生的亲本种染色体组分开现象提供了分子细胞遗传学证据. 展开更多
关键词 纯合植株 基因组原位杂交 芸苔属 诸葛菜 非整倍体 属间杂种 染色体组分开 植物遗传育种 油料植物
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High efficiency production and genomic in situ hybridization analysis of Brassica aneuploids and homozygous plants 被引量:1
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作者 李再云 m.ceccarelli +3 位作者 S.Minelli A.Contento 刘焰 P.G.Cionini 《Science China(Life Sciences)》 SCIE CAS 2003年第1期104-112,共10页
Interspecific and intergeneric hybridizations have been widely used in plant genetics and breeding to construct stocks for genetic analysis and to introduce into crops the desirable traits and genes from their relativ... Interspecific and intergeneric hybridizations have been widely used in plant genetics and breeding to construct stocks for genetic analysis and to introduce into crops the desirable traits and genes from their relatives. The intergeneric crosses between Brassica juncea (L.) Czern. & Coss., B. carinata A. Braun and Orychophragmus violaceus (L.) O. E. Schulz were made and the plants produced were subjected to genomic in situ hybridization analysis. The mixoploids from the cross with B. juncea were divided into three groups. The partially fertile mixoploids in the first group (2n = 36—42) mainly contained the somatic cells and pollen mother cells (PMCs) with the 36 chromosomes of B. juncea and additional chromosomes of O. violaceus. The mixoploids (2n = 30—36) in the second and third groups were morphologically quite similar to the mother plants B. juncea and showed nearly normal fertility. The plants in the second group produced the majority of PMCs (2n = 36) with their chromosomes paired and segregated normally, but 1—4 pairs of the O. violaceus chromosomes were included in some PMCs. The plants in the third group produced only PMCs with the 36 B. juncea chromosomes, which were paired and segregated normally. The mixoploids (2n = 29—34) from the cross with B. carinata produced the majority of PMCs (2n = 34) with normal chromosome pairing and segregation, but some plants had some PMCs with 1—3 pairs of chromosomes from O. violaceus and other plants had only PMCs with the B. carinata chromosomes. The Brassica homozygous plants and aneuploids with complete or partial chromo-some complements of Brassica parents and various numbers of O. violaceus chromosomes were derived from these progeny plants. The results in this study provided the molecular cytogenetic evidence for the separation of parental genomes which was previously proposed to occur in the hybridizations of these two genera. 展开更多
关键词 BRASSICA species Orychophragmus violaceus aneuploids INTERGENERIC hybrids GENOME separation.
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Containment effort reduction and regrowth patterns of the Covid-19 spreading
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作者 D.Lanteri D.Carco +2 位作者 P.Castorina m.ceccarelli B.Cacopardo 《Infectious Disease Modelling》 2021年第1期632-642,共11页
In all countries the political decisions aim to achieve an almost stable configuration with a small number of new infected individuals per day due to Covid-19.When such a condition is reached,the containment effort is... In all countries the political decisions aim to achieve an almost stable configuration with a small number of new infected individuals per day due to Covid-19.When such a condition is reached,the containment effort is usually reduced in favor of a gradual reopening of the social life and of the various economical sectors.However,in this new phase,the infection spread restarts and,moreover,possible mutations of the virus give rise to a large specific growth rate of the infected people.Therefore,a quantitative analysis of the regrowth pattern is very useful.We discuss a macroscopic approach which,on the basis of the collected data in the first lockdown,after few days from the beginning of the new phase,outlines different scenarios of the Covid-19 diffusion for longer time.The purpose of this paper is a demonstration-of-concept:one takes simple growth models,considers the available data and shows how the future trend of the spread can be obtained.The method applies a time dependent carrying capacity,analogously to many macroscopic growth laws in biology,economics and population dynamics.The illustrative cases of France,Italy and United Kingdom are analyzed. 展开更多
关键词 Covid-19 spreading Mathematical models Macroscopic growth laws Carrying capacity
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