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浙江和河北发生的一种水稻、小麦、玉米矮缩病是水稻黑条矮缩病毒引起的(英文) 被引量:85
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作者 张恒木 雷娟利 +4 位作者 陈剑平 吕永平 陈声祥 薛庆中 m.j.adams 《中国病毒学》 CSCD 2001年第3期246-251,共6页
以浙江省的水稻黑条矮缩病和河北省的玉米粗缩病的病毒分离物为材料 ,对我国南北方两种病毒分离物进行了比较研究。两种病毒分离物的粒子大小形态相似 ,且在血清学上密切相关 ;二者的介体、寄主相同 ,并引起相同的症状。提纯两种病毒分... 以浙江省的水稻黑条矮缩病和河北省的玉米粗缩病的病毒分离物为材料 ,对我国南北方两种病毒分离物进行了比较研究。两种病毒分离物的粒子大小形态相似 ,且在血清学上密切相关 ;二者的介体、寄主相同 ,并引起相同的症状。提纯两种病毒分离物的基因组片段凝胶电泳显示它们相应的基因组片段之间大小极其相似。根据水稻黑条矮缩病毒 (Riceblackstreakeddwarfvirus) ,RBSDV的S7和S8设计引物 ,利用RT PCR技术 ,在两种病毒分离物中均可特异扩增到预期大小的片段。序列测定比较分析表明 :它们的同源性达 97.0 %~ 98.9% ,与日本RBSDV的同源性 (92 .2 %~ 95 .5 % )高于与意大利MRDV的同源性 (76 .6 %~ 88.4% )。从而认为我国南方的水稻黑条矮缩病和北方和玉米粗缩病都是同一种病毒———RBSDV引起的 ,也就是说我国北方的玉米粗缩病病原实际上是水稻黑条矮缩病毒 ,而不是玉米粗缩病毒。 展开更多
关键词 水稻黑条矮缩病毒 玉米粗缩病毒 核苷酸序列
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中国大麦黄花叶病毒分离物的分子变异 被引量:7
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作者 施农农 陈剑平 m.j.adams 《病毒学报》 CAS CSCD 北大核心 1999年第4期339-347,共9页
13 个供试的中国和英国大麦黄花叶病毒(BaYMV) 分离物,经RNA1 和RNA2 全基因组不同区域DNA片段单链构象多态性分析(SSCP) ,外壳蛋白基因和RNA2 70kD基因5'端705 碱基序列分析,以及此705 ... 13 个供试的中国和英国大麦黄花叶病毒(BaYMV) 分离物,经RNA1 和RNA2 全基因组不同区域DNA片段单链构象多态性分析(SSCP) ,外壳蛋白基因和RNA2 70kD基因5'端705 碱基序列分析,以及此705 碱基DNA 片段限制性内切酶图谱分析结果,它们的RNA1 和RNA2 彼此无一相同,其中RNA2 变异比RNA1 更大。由于变异十分复杂,且没有规律性,因而当前通用的分子生物学技术尚不能简单地用于BaYMV 致病性分化或株系区分和检测。 展开更多
关键词 大麦黄花叶病毒 分子变异 序列分析 RT-PCR SSCP
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意大利小麦真菌传棒状病毒与欧洲小麦花叶病毒和土传黑麦花叶病毒属同一种病毒 被引量:8
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作者 杨建平 陈剑平 +2 位作者 陈炯 程晔 m.j.adams 《中国科学(C辑)》 CSCD 2000年第6期627-634,共8页
测定了从意大利硬红小麦上分离的一个真菌传棒状病毒分离物两个RNA的核苷酸全序列, 并与美国的土传小麦花叶病毒(SBWMV)、最近发表的从法国小麦上分离的欧洲小麦花叶病毒(EWMV)和从德国黑麦和小麦上分离的3个土传黑麦花叶病毒(SBRMV-C,-... 测定了从意大利硬红小麦上分离的一个真菌传棒状病毒分离物两个RNA的核苷酸全序列, 并与美国的土传小麦花叶病毒(SBWMV)、最近发表的从法国小麦上分离的欧洲小麦花叶病毒(EWMV)和从德国黑麦和小麦上分离的3个土传黑麦花叶病毒(SBRMV-C,-G, -O)进行了序列比较和系统树分析. 结果表明, 意大利分离物的RNA1和RNA2分别由7 025和3 688个碱基组成, 与EWMV的同源性分别为97.5%和98.6%, 与SBRMV-G 为95.5%和85.8%, 而与SBWMV 仅为70.6%和64.5%. 因此, 意大利分离物与SBWMV不同, 但与EWMV和SBRMV同属一种病毒, 建议将这些欧洲病毒命名为土传禾谷类花叶病毒(SBCMV), 以避免与以前的名字混淆. 展开更多
关键词 真菌传棒状病毒 核苷酸序列 土传禾谷类花叶病毒
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Sequence analysis of a soil-borne wheat mosaic virus isolate from Italy shows that it is the same virus as European wheat mosaic virus and Soil-borne rye mosaic virus 被引量:1
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作者 杨建平 陈剑平 +2 位作者 陈炯 程晔 m.j.adams 《Science China(Life Sciences)》 SCIE CAS 2001年第2期216-224,共9页
The complete sequence of the two RNAs of a furovirus isolate fromdurum wheat in Italy was determined. Sequence comparisons and phylogenetic analysis were done to compare the Italian virus with Soil-borne wheat mosaic ... The complete sequence of the two RNAs of a furovirus isolate fromdurum wheat in Italy was determined. Sequence comparisons and phylogenetic analysis were done to compare the Italian virus with Soil-borne wheat mosaic virus (SBWMV) from the USA and with furovirus sequences recently published as European wheat mosaic virus (EWMV), from wheat in France, and Soil-borne rye mosaic virus (SBRMV), from rye and wheat in Germany. Over the entire genome, the Italian isolate RNA1 and RNA2 had respectively 97.5% and 98.6% nucleotide identity with EWMV, 95.5% and 85.8% with SBRMV-G and 70.6% and 64.5% with SBWMV. The Italian isolate was therefore clearly distinct from SBWMV. The European isolates all appear to belong to the same virus and the name Soil-borne cereal mosaic virus may resolve earlier ambiguities. 展开更多
关键词 furovirus nucleotide sequence taxonomy.
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Modulation properties of solitary and optically injected phased-array semiconductor lasers
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作者 NIANQIANG LI H.SUSANTO +2 位作者 B.R.CEMLYN I.D.HENNING m.j.adams 《Photonics Research》 SCIE EI 2018年第9期908-917,共10页
We study modulation properties of two-element phased-array semiconductor lasers that can be described by coupled mode theory. We consider four different waveguide structures and modulate the array either in phase or o... We study modulation properties of two-element phased-array semiconductor lasers that can be described by coupled mode theory. We consider four different waveguide structures and modulate the array either in phase or out of phase within the phase-locked regions, guided by stability diagrams obtained from direct numerical simulations. Specifically, we find that out-of-phase modulation allows for bandwidth enhancement if the waveguide structure is properly chosen; for example, for a combination of index antiguiding and gain-guiding, the achievable modulation bandwidth in the case of out-of-phase modulation could be much higher than the one when they are modulated in phase. Proper array design of the coupling, controllable in terms of the laser separation and the frequency offset between the two lasers, is shown to be beneficial to slightly improve the bandwidth but not the resonance frequency, while the inclusion of the frequency offset leads to the appearance of double peak response curves. For comparison, we explore the case of modulating only one element of the phased array and find that double peak response curves are found. To improve the resonance frequency and the modulation bandwidth, we introduce simultaneous external injection into the phased array and modulate the phased array or its master light within the injection locking region. We observe a significant improvement of the modulation properties, and in some cases, by modulating the amplitude of the master light before injection,the resulting 3 dB bandwidths could be enhanced up to 160 GHz. Such a record bandwidth for phased-array modulation could pave the way for various applications, notably optical communications that require high-speed integrated photonic devices. 展开更多
关键词 半导体激光器 发展现状 通讯技术 技术创新
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