Based on experimental data from hydrocarbon generation with a semi-open system, hydrocarbon generation kinetics modeling in gold tube of closed system, high temperature pyrolysis chromatography mass spectrometry exper...Based on experimental data from hydrocarbon generation with a semi-open system, hydrocarbon generation kinetics modeling in gold tube of closed system, high temperature pyrolysis chromatography mass spectrometry experiment with open system and geological data, the characteristics of whole hydrocarbon-generating process, hydrocarbon expulsion efficiency and retained hydrocarbon quantity, origins of natural gas generated in high-over mature stage and cracking temperature of methane homologs were investigated in this study. The sapropelic source rock has a hydrocarbon expulsion efficiency of 30%-60% and 60%-80% in the major oil generation window(with Ro of 0.8%-1.3%) and high maturity stage(with Ro of 1.3%-2.0%) respectively; and the contribution ratio of kerogen degradation gas to oil cracking gas in total generated gas in high maturity stage is about 1:4. The degradation gas of kerogen accounts for 20%, the retained liquid hydrocarbon cracking gas accounts for 13.5%, and the amount of out-reservoir oil cracking gas(including aggregation type and dispersed oil cracking gas) accounts for 66.5%. The lower limit of gas cracking is determined preliminarily. Based on the new understandings, a model of the whole hydrocarbon-generating process of source rock is built.展开更多
We have combined the analytical data of the carbon isotope distribution pattern, R/Ra and CH4/3He values of abiogenic and biogenic (referring to the thermogenic and bacterial or microbial) alkane gases in China with t...We have combined the analytical data of the carbon isotope distribution pattern, R/Ra and CH4/3He values of abiogenic and biogenic (referring to the thermogenic and bacterial or microbial) alkane gases in China with those of alkane gases from USA, Russia, Germany, Australia and other countries. Four discrimination criteria are derived from this comparative study: 1) Carbon isotopic composition is generally greater than ?30‰ for abiogenic methane and less than ?30‰ for biogenic methane; 2) Abiogenic alkane gases have a carbon isotopic reversal trend (δ13C1> δ13C2> δ13C3> δ13C4) with δ13C1> ?30‰ in general; 3) Gases with R/Ra >0.5 and δ13C1? δ13C2>0 are of abiogenic origin; 4) Gases (methane) with CH4/3He?106 are of abiogenic origin, whereas gases with CH4/3He?1011 are of biogenic origin.展开更多
文摘目的:探讨流体剪切力(FSS)对Piezo1的影响以及调控MG-63细胞凋亡的机制。方法:首先分两组Control和FSS组,FSS组施加FSS,Control组不干预,使用Western Blot检测两组caspase3和Piezo1的表达。再将细胞分为Control组、FSS组、GsMTx4组和FSS+GsMTx4组,FSS组加载FSS,GsMTx4组给予GsMTx4处理,FSS+GsMTx4组先给予GsMTx4处理后再加载FSS,检测各组凋亡相关蛋白caspase3、caspase9、BAD和Bax的表达水平。结果:60 min 12 dyn/cm^(2)的FSS可上调MG-63细胞caspase3和Piezo1的表达,此外,FSS可通过上调Piezo1的水平从而促进caspase3、caspase9、BAD和Bax的表达水平。结论:FSS可通过Piezo1机械敏感离子通道促进MG-63人骨肉瘤细胞的凋亡,Piezo1可能是一种治疗骨肉瘤的新型分子靶点。
基金Supported by the National Science and Technology Major Project(2016ZX05007-003,2011ZX05007-002)
文摘Based on experimental data from hydrocarbon generation with a semi-open system, hydrocarbon generation kinetics modeling in gold tube of closed system, high temperature pyrolysis chromatography mass spectrometry experiment with open system and geological data, the characteristics of whole hydrocarbon-generating process, hydrocarbon expulsion efficiency and retained hydrocarbon quantity, origins of natural gas generated in high-over mature stage and cracking temperature of methane homologs were investigated in this study. The sapropelic source rock has a hydrocarbon expulsion efficiency of 30%-60% and 60%-80% in the major oil generation window(with Ro of 0.8%-1.3%) and high maturity stage(with Ro of 1.3%-2.0%) respectively; and the contribution ratio of kerogen degradation gas to oil cracking gas in total generated gas in high maturity stage is about 1:4. The degradation gas of kerogen accounts for 20%, the retained liquid hydrocarbon cracking gas accounts for 13.5%, and the amount of out-reservoir oil cracking gas(including aggregation type and dispersed oil cracking gas) accounts for 66.5%. The lower limit of gas cracking is determined preliminarily. Based on the new understandings, a model of the whole hydrocarbon-generating process of source rock is built.
基金the Science and Technology Project of PetroChina (Grant No.07-01C-01-07)
文摘We have combined the analytical data of the carbon isotope distribution pattern, R/Ra and CH4/3He values of abiogenic and biogenic (referring to the thermogenic and bacterial or microbial) alkane gases in China with those of alkane gases from USA, Russia, Germany, Australia and other countries. Four discrimination criteria are derived from this comparative study: 1) Carbon isotopic composition is generally greater than ?30‰ for abiogenic methane and less than ?30‰ for biogenic methane; 2) Abiogenic alkane gases have a carbon isotopic reversal trend (δ13C1> δ13C2> δ13C3> δ13C4) with δ13C1> ?30‰ in general; 3) Gases with R/Ra >0.5 and δ13C1? δ13C2>0 are of abiogenic origin; 4) Gases (methane) with CH4/3He?106 are of abiogenic origin, whereas gases with CH4/3He?1011 are of biogenic origin.
