The effect of Oxytropis glabra DC. on α-mannosidase( AMA) expression in mice brain tissue was explored to reveal the toxicity mechanism of O. glabra. Forty mice were randomly divided into four groups,namely control g...The effect of Oxytropis glabra DC. on α-mannosidase( AMA) expression in mice brain tissue was explored to reveal the toxicity mechanism of O. glabra. Forty mice were randomly divided into four groups,namely control group,experimental group I,experimental group II and experimental group III. The mice in three experimental groups were fed with O. glabra at the doses of 1,5 and 10 g per kilogram weight,respectively. After challenge for 63 d,mice brains were collected to detect changes in distribution and expression of AMA in different brain regions. The results showed that O. glabra poisoning led to declined AMA mRNA expression in mice brain tissue,but the mice in experimental group I had no significant difference with those in control group( P > 0. 05). The AMA mRNA expression in cerebellum,cerebrum and thalamus of mice in experimental groups II and III were significantly lower than that in control group( P < 0. 05),but the AMA mRNA expression in hippocampus and brainstem in three experimental groups had no significant difference with that in control group( P > 0. 05). AMA had very weak expression in hippocampus and brainstem,but it had expressions in other regions,and the expression was positively correlated with the number of neurons and granulosa cells. The results showed that different doses of O. glabra reduced AMA mRNA expression in mice brain tissue,while cerebellum,cerebrum and thalamus were the main target function areas.展开更多
基金Supported by National Natural Science Foundation of China(31460678)Open Project of Key Laboratory of Tarim Animal Husbandry Science and Technology in Xinjiang Production and Construction Corps(HS201409)
文摘The effect of Oxytropis glabra DC. on α-mannosidase( AMA) expression in mice brain tissue was explored to reveal the toxicity mechanism of O. glabra. Forty mice were randomly divided into four groups,namely control group,experimental group I,experimental group II and experimental group III. The mice in three experimental groups were fed with O. glabra at the doses of 1,5 and 10 g per kilogram weight,respectively. After challenge for 63 d,mice brains were collected to detect changes in distribution and expression of AMA in different brain regions. The results showed that O. glabra poisoning led to declined AMA mRNA expression in mice brain tissue,but the mice in experimental group I had no significant difference with those in control group( P > 0. 05). The AMA mRNA expression in cerebellum,cerebrum and thalamus of mice in experimental groups II and III were significantly lower than that in control group( P < 0. 05),but the AMA mRNA expression in hippocampus and brainstem in three experimental groups had no significant difference with that in control group( P > 0. 05). AMA had very weak expression in hippocampus and brainstem,but it had expressions in other regions,and the expression was positively correlated with the number of neurons and granulosa cells. The results showed that different doses of O. glabra reduced AMA mRNA expression in mice brain tissue,while cerebellum,cerebrum and thalamus were the main target function areas.
文摘针对滚石运动特征参数间关系模糊等问题,在开展其敏感性分析的基础上,构建准确、快捷的滚石运动特征参数预测系统。首先引入灰色关联法(grey relation analysis,GRA),以水平运动距离、弹跳高度和碰撞能量等滚石运动特征为目标参数,研究了初始速度、边坡摩擦角、滚石质量、法向恢复系数和切向恢复系数等滚石运动影响因素的敏感性和显著性。随后,使用高斯过程回归模型,建立水平运动距离、弹跳高度和碰撞能量等滚石运动特征参数的预测模型,避免了传统的滚石运动数值模拟软件繁琐的建模计算过程。研究表明,高斯过程回归(Gaussian process regression,GPR)模型对滚石的水平距离和总动能的预测精度最高。因此,文中所构建的滚石运动特征参数敏感性分析及预测模型能够快速得出滚石运动特征参数,对支挡结构的设计和施工有重要的参考价值。
