[Objective] To amplify ATPase8 genes from genomic DNA of Guyuan chicken （white plume, ma plume and red plume）, Arbor acres broiler and Roman layer. [Method] Using specific pdmers for differential detection of chicke...[Objective] To amplify ATPase8 genes from genomic DNA of Guyuan chicken （white plume, ma plume and red plume）, Arbor acres broiler and Roman layer. [Method] Using specific pdmers for differential detection of chicken-derived ingredients, the ATPase8 genes were amplified from the genomic DNA extracted from chicken blood by PCR. And the PCR products were sequenced. [ Result] The full length sequence of chicken ATPase8 was 165 bp in the Guyuan chicken and Arbor acres broiler and 168 bp in Roman layer. [Condusion] The ATPase8 gene of the Guyuan chicken has high homology to that of the Arbor acres broiler or Roman layer, but it has low homology to that of quail, partridge, ostrich, goose or turkey.展开更多
基金funded by the Natural Science Foundation of Ningxia of China (NZ0948)
文摘[Objective] To amplify ATPase8 genes from genomic DNA of Guyuan chicken （white plume, ma plume and red plume）, Arbor acres broiler and Roman layer. [Method] Using specific pdmers for differential detection of chicken-derived ingredients, the ATPase8 genes were amplified from the genomic DNA extracted from chicken blood by PCR. And the PCR products were sequenced. [ Result] The full length sequence of chicken ATPase8 was 165 bp in the Guyuan chicken and Arbor acres broiler and 168 bp in Roman layer. [Condusion] The ATPase8 gene of the Guyuan chicken has high homology to that of the Arbor acres broiler or Roman layer, but it has low homology to that of quail, partridge, ostrich, goose or turkey.
