Onshore Contribution to the Microbial Communities in the Lakes on the Qing-Tibetan Plateau

The inland lakes on the Tibetan Plateau are inhabited by diverse microbial communities,which play a significant role in biogeochemical cycles of biogenic elements(e.g.carbon,nitrogen).Onshore inputs provide essential

Microbial Succession in Response to Salinity in the Sediments of Lakes on the Northeastern Qinghai-Tibetan Plateau,China

Salinity is an important factor controlling the microbial distribution in lakes.However little is known about how microbial communities evolve in response to salinity variation.In this study,we investigated the microbial

C2H2流量对阴极弧沉积AlTiCN涂层结构和性能的影响

采用阴极弧技术制备了AlTiCN纳米复合薄膜,利用场发射电镜、X射线光电子能谱仪、纳米压痕仪、球盘式摩擦磨损实验机和电化学工作站研究了C2H2流量对AlTiCN薄膜微结构、硬度、摩擦特性及耐腐蚀性能的影响。结果表明:AlTi(C,N)薄膜在C2H2流量为30 SCCM时表现为粗大的柱状结构,随C2H2流量增大至60 SCCM,薄膜由柱状转为均匀的细晶结构。AlTi(C,N)薄膜在C2H2流量为60 SCCM时硬度达到最大值22.31 GPa,较高的硬度主要源于基体有足够多数量的AlTiCN晶粒的支撑。薄膜摩擦系数随C2H2流量的增加而减小。所沉积的样品在3.5wt%NaCl溶液中均展现出较好的耐腐蚀性。

芒果炭疽病病原亚洲炭疽菌(Colletotrichum asianum)特异性PCR检测

亚洲炭疽菌(Colletotrichum asianum)是一种能引发芒果炭疽病的优势病原菌。为建立一种能快速诊断该病原菌所引起的芒果炭疽病的PCR检测方法,本研究通过比较不同种类炭疽菌的全基因组DNA序列,获得了1条C.asianum特异性序列,设计并筛选得到1对特异性引物:caf1(5′-CCGTCAGACGGAATTATCAGC-3′)和car1(5′-CCGATCCTGTCTTTGAAATGG-3′)。结果表明,引物caf1/car1可以从C.asianum中扩增出长度为419 bp的特异性目标条带,灵敏度可达到1 pg·μL-1。利用该引物能够从人工接种C.asianum YN55-1的芒果叶片以及田间发生炭疽病的芒果叶片样品中快速稳定地检测出长度为419 bp的目标条带。本研究建立的特异性PCR检测方法为C.asianum导致的芒果炭疽病的田间监测和快速诊断提供了技术支持。