为了进一步研究水稻Catalase b (OsCATb)在重金属逆境胁迫下的作用机制,笔者将外源表达重组质粒pGEX-6p-3-OsCATb转化到大肠杆菌BL21中。SDS-PAGE表明,通过IPTG的诱导表达,在53 k Da的位置获得了与推测分子量大小相一致的可溶性目的蛋...为了进一步研究水稻Catalase b (OsCATb)在重金属逆境胁迫下的作用机制,笔者将外源表达重组质粒pGEX-6p-3-OsCATb转化到大肠杆菌BL21中。SDS-PAGE表明,通过IPTG的诱导表达,在53 k Da的位置获得了与推测分子量大小相一致的可溶性目的蛋白。在1 mmol/L Cd、Zn和Cu的重金属逆境胁迫下,过量表达GST-OsCATb的重组菌表现出优于对照菌株(BL21)以及过量表达标签蛋白GST的空载体菌株的耐受性;同时,目的基因重组菌体内的过氧化氢酶活性也要远高于实验对照组。研究结果也进一步暗示了水稻OsCATb作为重要的抗氧化剂,能够参与到细胞的重金属逆境胁迫应答、解毒和耐受机制过程中。展开更多
In this paper,a cooperative region reconnaissance problem is investigated where a group of agents are required to fly across and detect events occur in an environment with static obstacles until an effective coverage ...In this paper,a cooperative region reconnaissance problem is investigated where a group of agents are required to fly across and detect events occur in an environment with static obstacles until an effective coverage is achieved.First,the region reconnaissance is formulated as a non-convex optimization problem.A coverage performance index with additional collision and obstacle avoidance constraints is given.Since the optimization index is an implicit function of state variables and cannot be used to compute gradients on state variables directly,an approximate optimization index is selected.Then,a non-convex optimization-based coverage algorithm is proposed to find the optimal reconnaissance location for each agent and guarantee no collisions trajectories among agents and obstacles.Finally,simulation experiments are performed to verify the effectiveness of the proposed approach.展开更多
文摘为了进一步研究水稻Catalase b (OsCATb)在重金属逆境胁迫下的作用机制,笔者将外源表达重组质粒pGEX-6p-3-OsCATb转化到大肠杆菌BL21中。SDS-PAGE表明,通过IPTG的诱导表达,在53 k Da的位置获得了与推测分子量大小相一致的可溶性目的蛋白。在1 mmol/L Cd、Zn和Cu的重金属逆境胁迫下,过量表达GST-OsCATb的重组菌表现出优于对照菌株(BL21)以及过量表达标签蛋白GST的空载体菌株的耐受性;同时,目的基因重组菌体内的过氧化氢酶活性也要远高于实验对照组。研究结果也进一步暗示了水稻OsCATb作为重要的抗氧化剂,能够参与到细胞的重金属逆境胁迫应答、解毒和耐受机制过程中。
基金partially supported by the National Natural Science Foundation of China under Grant Nos.6147309961333001。
文摘In this paper,a cooperative region reconnaissance problem is investigated where a group of agents are required to fly across and detect events occur in an environment with static obstacles until an effective coverage is achieved.First,the region reconnaissance is formulated as a non-convex optimization problem.A coverage performance index with additional collision and obstacle avoidance constraints is given.Since the optimization index is an implicit function of state variables and cannot be used to compute gradients on state variables directly,an approximate optimization index is selected.Then,a non-convex optimization-based coverage algorithm is proposed to find the optimal reconnaissance location for each agent and guarantee no collisions trajectories among agents and obstacles.Finally,simulation experiments are performed to verify the effectiveness of the proposed approach.
