目的分析献血人群队列建立的相关研究,了解国外献血队列研究发展历程,发现当前国际上献血人群队列研究的研究现状、研究热点和发展趋势。方法基于Web of Science核心合集数据库检索献血者队列相关文献,经过筛选和数据处理后,对纳入的文...目的分析献血人群队列建立的相关研究,了解国外献血队列研究发展历程,发现当前国际上献血人群队列研究的研究现状、研究热点和发展趋势。方法基于Web of Science核心合集数据库检索献血者队列相关文献,经过筛选和数据处理后,对纳入的文献从发文量趋势、学科、作者、机构分布进行文献计量分析,运用Citespace 5.6R5软件进行国家/地区、文献共被引、关键词共现、关键词聚类、关键词突现的可视化分析。结果最终纳入672篇文献(研究型论文654篇,综述类论文18篇),1991年至今献血者队列研究领域的发文量整体呈现平稳上升趋势,以2019年至2020年增长最为迅速。被引频次排名前3位的作者分别为Kaaks R(1301次)、Rinaldi S(1186次)、Riboli E(1130次);排名前3位的机构分别为英国RLUK图书馆、加州大学和哥本哈根大学;排名前3位的国家/地区分别是美国(176篇)、德国(64篇)、法国(54篇),且均与其他国家合作紧密。出现频次排名前5位的关键词分别为“blood donor(献血者)”“prevalence(流行情况)”“infection(感染)”“risk(风险)”“antibody(抗体)”。关键词聚类分析后共得到19个聚类,具体体现在经血传染疾病免疫机制以及流行情况、献血行为与非传染性疾病相关及其影响因素、献血人群特征分类3个方面。突现强度最大的关键词是“non B hepatitis(非乙型肝炎)”,最近几年突现且持续至今的有“donation(捐赠)”“seroprevalence(血清流行率)”“donor(献血者)”“management(管理)”。结论目前献血队列研究在国际上逐渐受到关注,其研究热点和趋势主要集中在输血传播疾病风险相关的研究,但也有越来越多的研究开始关注供血者或受血者本身的健康问题及影响因素,为我国开展献血队列研究提供了参考依据和研究方向。展开更多
该研究运用CiteSpace计量可视化分析软件,以CNKI和Web of Science数据库中高分子实验课程的文献为研究对象,通过作者合作、机构合作及关键词分析,揭示我国对高分子实验课程研究的现状.结果显示,国内的作者合作及机构合作相对于国外较为...该研究运用CiteSpace计量可视化分析软件,以CNKI和Web of Science数据库中高分子实验课程的文献为研究对象,通过作者合作、机构合作及关键词分析,揭示我国对高分子实验课程研究的现状.结果显示,国内的作者合作及机构合作相对于国外较为密切;国内的热点关键词为教学改革、高分子材料、应用型人才等,揭示国内的研究热点为实验教学改革、应用型人才的培养;国外的热点关键词为动手学习和操作、实验室建设等,说明国外研究热点是在提升学生动手操作能力等方面.展开更多
In this study,37 derivatives of phorbol esters were synthesized and their anti-HIV-1 activities evaluated,building upon our previous synthesis of 51 phorbol derivatives.12-Para-electron-acceptor-trans-cinnamoyl-13-dec...In this study,37 derivatives of phorbol esters were synthesized and their anti-HIV-1 activities evaluated,building upon our previous synthesis of 51 phorbol derivatives.12-Para-electron-acceptor-trans-cinnamoyl-13-decanoyl phorbol derivatives stood out,demonstrating remarkable anti-HIV-1 activities and inhibitory effects on syncytia formation.These derivatives exhibited a higher safety index compared with the positive control drug.Among them,12-(trans-4-fluorocinnamoyl)-13-decanoyl phorbol,designated as compound 3c,exhibited the most potent anti-HIV-1 activity(EC_(50)2.9 nmol·L^(−1),CC50/EC_(50)11117.24)and significantly inhibited the formation of syncytium(EC_(50)7.0 nmol·L^(−1),CC50/EC_(50)4891.43).Moreover,compound 3c is hypothesized to act both as an HIV-1 entry inhibitor and as an HIV-1 reverse transcriptase inhibitor.Isothermal titration calorimetry and molecular docking studies indicated that compound 3c may also function as a natural activator of protein kinase C(PKC).Therefore,compound 3c emerges as a potential candidate for developing new anti-HIV drugs.展开更多
Phorbol esters are recognized for their dual role as anti-HIV-1 agents and as activators of protein kinase C(PKC).The efficacy of phorbol esters in binding with PKC is attributed to the presence of oxygen groups at po...Phorbol esters are recognized for their dual role as anti-HIV-1 agents and as activators of protein kinase C(PKC).The efficacy of phorbol esters in binding with PKC is attributed to the presence of oxygen groups at positions C20,C3/C4,and C9 of phorbol.Concurrently,the lipids located at positions C12/C13 are essential for both the anti-HIV-1 activity and the formation of the PKC-ligand complex.The influence of the cyclopropane ring at positions C13 and C14 in phorbol derivatives on their anti-HIV-1 activity requires further exploration.This research entailed the hydrolysis of phorbol,producing seco-cyclic phorbol derivatives.The anti-HIV-1 efficacy of these derivatives was assessed,and the affinity constant(Kd)for PKC-δprotein of selected seco-cyclic phorbol derivatives was determined through isothermal titration calorimetry.The findings suggest that the chemical modification of cyclopropanols could affect both the anti-HIV-1 activity and the PKC binding affinity.Remarkably,compound S11,with an EC_(50) of 0.27μmol·L^(−1) and a CC_(50) of 153.92μmol·L^(−1),demonstrated a potent inhibitory effect on the intermediate products of HIV-1 reverse transcription(ssDNA and 2LTR),likely acting at the viral entry stage,yet showed no affinity for the PKC-δprotein.These results position compound S11 as a potential candidate for further preclinical investigation and for studies aimed at elucidating the pharmacological mechanism underlying its anti-HIV-1 activity.展开更多
文摘目的分析献血人群队列建立的相关研究,了解国外献血队列研究发展历程,发现当前国际上献血人群队列研究的研究现状、研究热点和发展趋势。方法基于Web of Science核心合集数据库检索献血者队列相关文献,经过筛选和数据处理后,对纳入的文献从发文量趋势、学科、作者、机构分布进行文献计量分析,运用Citespace 5.6R5软件进行国家/地区、文献共被引、关键词共现、关键词聚类、关键词突现的可视化分析。结果最终纳入672篇文献(研究型论文654篇,综述类论文18篇),1991年至今献血者队列研究领域的发文量整体呈现平稳上升趋势,以2019年至2020年增长最为迅速。被引频次排名前3位的作者分别为Kaaks R(1301次)、Rinaldi S(1186次)、Riboli E(1130次);排名前3位的机构分别为英国RLUK图书馆、加州大学和哥本哈根大学;排名前3位的国家/地区分别是美国(176篇)、德国(64篇)、法国(54篇),且均与其他国家合作紧密。出现频次排名前5位的关键词分别为“blood donor(献血者)”“prevalence(流行情况)”“infection(感染)”“risk(风险)”“antibody(抗体)”。关键词聚类分析后共得到19个聚类,具体体现在经血传染疾病免疫机制以及流行情况、献血行为与非传染性疾病相关及其影响因素、献血人群特征分类3个方面。突现强度最大的关键词是“non B hepatitis(非乙型肝炎)”,最近几年突现且持续至今的有“donation(捐赠)”“seroprevalence(血清流行率)”“donor(献血者)”“management(管理)”。结论目前献血队列研究在国际上逐渐受到关注,其研究热点和趋势主要集中在输血传播疾病风险相关的研究,但也有越来越多的研究开始关注供血者或受血者本身的健康问题及影响因素,为我国开展献血队列研究提供了参考依据和研究方向。
文摘该研究运用CiteSpace计量可视化分析软件,以CNKI和Web of Science数据库中高分子实验课程的文献为研究对象,通过作者合作、机构合作及关键词分析,揭示我国对高分子实验课程研究的现状.结果显示,国内的作者合作及机构合作相对于国外较为密切;国内的热点关键词为教学改革、高分子材料、应用型人才等,揭示国内的研究热点为实验教学改革、应用型人才的培养;国外的热点关键词为动手学习和操作、实验室建设等,说明国外研究热点是在提升学生动手操作能力等方面.
基金supported by the National Natural Science Foundation of China(Nos.81202882,82060670)Suzhou Science and Technology Planning Project in Jiangsu Province of China(No.SNG2021022)+1 种基金the Priority Academic Program Development of the Jiangsu Higher Education Institutes,China(PAPD)and the Project of Innovative Research Team of Yunnan Province(No.202005AE160005).
文摘In this study,37 derivatives of phorbol esters were synthesized and their anti-HIV-1 activities evaluated,building upon our previous synthesis of 51 phorbol derivatives.12-Para-electron-acceptor-trans-cinnamoyl-13-decanoyl phorbol derivatives stood out,demonstrating remarkable anti-HIV-1 activities and inhibitory effects on syncytia formation.These derivatives exhibited a higher safety index compared with the positive control drug.Among them,12-(trans-4-fluorocinnamoyl)-13-decanoyl phorbol,designated as compound 3c,exhibited the most potent anti-HIV-1 activity(EC_(50)2.9 nmol·L^(−1),CC50/EC_(50)11117.24)and significantly inhibited the formation of syncytium(EC_(50)7.0 nmol·L^(−1),CC50/EC_(50)4891.43).Moreover,compound 3c is hypothesized to act both as an HIV-1 entry inhibitor and as an HIV-1 reverse transcriptase inhibitor.Isothermal titration calorimetry and molecular docking studies indicated that compound 3c may also function as a natural activator of protein kinase C(PKC).Therefore,compound 3c emerges as a potential candidate for developing new anti-HIV drugs.
基金supported by the National Natural Science Foundation of China(Nos.81202882 and 82060670)the Suzhou Science and Technology Planning Project in Jiangsu Province of China(No.SNG2021022)+1 种基金the Priority Academic Program Development of the Jiangsu Higher Education Institutes,China(PAPD)Project of Innovative Research Team of Yunnan Province(No.202005AE160005)。
文摘Phorbol esters are recognized for their dual role as anti-HIV-1 agents and as activators of protein kinase C(PKC).The efficacy of phorbol esters in binding with PKC is attributed to the presence of oxygen groups at positions C20,C3/C4,and C9 of phorbol.Concurrently,the lipids located at positions C12/C13 are essential for both the anti-HIV-1 activity and the formation of the PKC-ligand complex.The influence of the cyclopropane ring at positions C13 and C14 in phorbol derivatives on their anti-HIV-1 activity requires further exploration.This research entailed the hydrolysis of phorbol,producing seco-cyclic phorbol derivatives.The anti-HIV-1 efficacy of these derivatives was assessed,and the affinity constant(Kd)for PKC-δprotein of selected seco-cyclic phorbol derivatives was determined through isothermal titration calorimetry.The findings suggest that the chemical modification of cyclopropanols could affect both the anti-HIV-1 activity and the PKC binding affinity.Remarkably,compound S11,with an EC_(50) of 0.27μmol·L^(−1) and a CC_(50) of 153.92μmol·L^(−1),demonstrated a potent inhibitory effect on the intermediate products of HIV-1 reverse transcription(ssDNA and 2LTR),likely acting at the viral entry stage,yet showed no affinity for the PKC-δprotein.These results position compound S11 as a potential candidate for further preclinical investigation and for studies aimed at elucidating the pharmacological mechanism underlying its anti-HIV-1 activity.
