IM To investigate the agerelated alterations of cytoskeleton system in liver Kupffer cell and their relation to the changed phagocytic function.METHODS The phagocytic function of Kupffer cells from rats of various a...IM To investigate the agerelated alterations of cytoskeleton system in liver Kupffer cell and their relation to the changed phagocytic function.METHODS The phagocytic function of Kupffer cells from rats of various ages (6mo, 12mo, 18mo and 24mo) were quantitatively evaluated by phagocytosis of polystyrene beads. The actin distribution and measurement of Kupffer cell were determined by a phalloidinTRITC method; and the myosin and vimentin distribution and measurement with indirect immunochemical staining.RESULTS Aging resulted in significant alterations of actin, myosin and vimentin distributions and reductions in Kupffer cell; the 3 cytoskeleton components of 24moold Kupffer cell were significantly decreased to 680%, 849% and 755%, respectively of these of 6moold Kupffer cell(P<001,001 and 001). And these decreases had significant positive relations with the damaged phagocytosis of the aged Kupffer cell. γ values were 096(P<005), 099(P<001) and 095 (P<005) respectively.CONCLUSION The cytoskeleton system of the aged Kupffer cell presents an evident state of senescence, which may be an important mechanism of decreased phagocytosis of the aged Kupffer cell..展开更多
文摘IM To investigate the agerelated alterations of cytoskeleton system in liver Kupffer cell and their relation to the changed phagocytic function.METHODS The phagocytic function of Kupffer cells from rats of various ages (6mo, 12mo, 18mo and 24mo) were quantitatively evaluated by phagocytosis of polystyrene beads. The actin distribution and measurement of Kupffer cell were determined by a phalloidinTRITC method; and the myosin and vimentin distribution and measurement with indirect immunochemical staining.RESULTS Aging resulted in significant alterations of actin, myosin and vimentin distributions and reductions in Kupffer cell; the 3 cytoskeleton components of 24moold Kupffer cell were significantly decreased to 680%, 849% and 755%, respectively of these of 6moold Kupffer cell(P<001,001 and 001). And these decreases had significant positive relations with the damaged phagocytosis of the aged Kupffer cell. γ values were 096(P<005), 099(P<001) and 095 (P<005) respectively.CONCLUSION The cytoskeleton system of the aged Kupffer cell presents an evident state of senescence, which may be an important mechanism of decreased phagocytosis of the aged Kupffer cell..