A multifunctional drug delivery system（GNRs@mSiO_2-HA-RGD） was developed by conjugating targeting ligand hyaluronic acid（HA） and RGD with mesoporous silica-coated gold nanorods（GNRs@mSiO_2） for dual-targeted che...A multifunctional drug delivery system（GNRs@mSiO_2-HA-RGD） was developed by conjugating targeting ligand hyaluronic acid（HA） and RGD with mesoporous silica-coated gold nanorods（GNRs@mSiO_2） for dual-targeted chemo-photothermal therapy. The physiochemical properties of the prepared nanoparticles were characterized by FTIR, UV-vis spectra, and ~1H NMR. Doxorubicin hydrochloride（DOX）, an anticancer drug, was used as the model drug to investigate the drug loading, in vitro drug release profiles and cytotoxicity. The experimental results show that DOX-GNRs@mSiO_2-HA-RGD is synthesized with a mean diameter of 116 nm and a sufficient load capacity of about 19.8%. It also has p H-enzyme sensitive and NIRtriggered drug release manner. Cellular uptake indicates that DOX-GNRs@mSiO_2-HA-RGD exhibits a higher cellular uptake via CD44 receptor and integrin receptor mediated endocytosis compared with the GNRs@mSiO_2 modified with one receptor or no receptor. In comparison with chemotherapy or photothermal therapy alone, DOX-GNRs@mSiO_2-HA-RGD displayes the synergistic effects and achieves a higher therapeutic efficacy. It can be expected that DOX-GNRs@mSiO_2-HA-RGD is a potential dual-targeted chemo-photothermal therapeutic platform for effective cancer treatment.展开更多
The unique topography and historical and cultural background have determined the diversity and uniqueness of kiln architecture in the Tongchuan area.In addition to the double-slope residential architecture,traditional...The unique topography and historical and cultural background have determined the diversity and uniqueness of kiln architecture in the Tongchuan area.In addition to the double-slope residential architecture,traditional kiln dwellings with regional characteristics such as Leaning on the cliff cave dwelling,ground Pit cave dwelling and Freestanding cave dwellings have also been formed.This paper takes the inheritance and protection of traditional kiln as the starting point,and through field research and literature analysis,we have systematically collected images,measured data,and drawn up horizontal and vertical profiles and three-dimensional structure drawings of the traditional kiln dwellings in Tongchuan,and concluded the three types of forms and structural characteristics and artistic form characteristics of the traditional kiln dwellings in Tongchuan.The aim is to provide a basis and reference for the protection and inheritance of tangible and intangible cultural heritage in Shaanxi,as well as for subsequent research in this field.展开更多
基金Funded by the National Natural Science Foundation of China(Nos.51473130 and 51572206)the Wuhan Huanghe excellence plan and Entrepreneurship Training Program of Wuhan University and Technology(Nos.20171049720018,20171049720019,and 20171049720009)
文摘A multifunctional drug delivery system（GNRs@mSiO_2-HA-RGD） was developed by conjugating targeting ligand hyaluronic acid（HA） and RGD with mesoporous silica-coated gold nanorods（GNRs@mSiO_2） for dual-targeted chemo-photothermal therapy. The physiochemical properties of the prepared nanoparticles were characterized by FTIR, UV-vis spectra, and ~1H NMR. Doxorubicin hydrochloride（DOX）, an anticancer drug, was used as the model drug to investigate the drug loading, in vitro drug release profiles and cytotoxicity. The experimental results show that DOX-GNRs@mSiO_2-HA-RGD is synthesized with a mean diameter of 116 nm and a sufficient load capacity of about 19.8%. It also has p H-enzyme sensitive and NIRtriggered drug release manner. Cellular uptake indicates that DOX-GNRs@mSiO_2-HA-RGD exhibits a higher cellular uptake via CD44 receptor and integrin receptor mediated endocytosis compared with the GNRs@mSiO_2 modified with one receptor or no receptor. In comparison with chemotherapy or photothermal therapy alone, DOX-GNRs@mSiO_2-HA-RGD displayes the synergistic effects and achieves a higher therapeutic efficacy. It can be expected that DOX-GNRs@mSiO_2-HA-RGD is a potential dual-targeted chemo-photothermal therapeutic platform for effective cancer treatment.
基金National Social Science Foundation of the Arts Key Project“Research on the Architecture Art and Folk Culture of Chinese Traditional Houses on the Land‘Silk Road’(Number:18AH008)”One of the Periodic Achievements of the Project Entrusted by the Ministry of Culture and Tourism:“Yellow River Culture and Chinese Civilization:Rescue Research on Shaanxi Traditional Residential Buildings and Residential Folk Culture”(Project Approval No.21HH02).
文摘The unique topography and historical and cultural background have determined the diversity and uniqueness of kiln architecture in the Tongchuan area.In addition to the double-slope residential architecture,traditional kiln dwellings with regional characteristics such as Leaning on the cliff cave dwelling,ground Pit cave dwelling and Freestanding cave dwellings have also been formed.This paper takes the inheritance and protection of traditional kiln as the starting point,and through field research and literature analysis,we have systematically collected images,measured data,and drawn up horizontal and vertical profiles and three-dimensional structure drawings of the traditional kiln dwellings in Tongchuan,and concluded the three types of forms and structural characteristics and artistic form characteristics of the traditional kiln dwellings in Tongchuan.The aim is to provide a basis and reference for the protection and inheritance of tangible and intangible cultural heritage in Shaanxi,as well as for subsequent research in this field.
