Objective:To systematically evaluate the endometrial thickening and Chinese herbal medicine intervention effects during endocrine therapy following breast cancer surgery.Methods:Computerized searches were performed on...Objective:To systematically evaluate the endometrial thickening and Chinese herbal medicine intervention effects during endocrine therapy following breast cancer surgery.Methods:Computerized searches were performed on CNKI,CBM,Wanfang,VIP,Pub Med,Embase,and Cochrane to gather randomized controlled trials(RCTs)of endometrial thickening combined with Chinese herbal medicine intervention during endocrine therapy after breast cancer surgery.The meta analysis is done using Rev Man 5.4,and the retrieval range is from the database's creation to January 2023.Results:There were 710 patients enrolled in a total of 12 RCTs.The results of Meta-analysis were as follows:combined with Chinese herbal medicine treatment for 3 months of endometrial thickness(MD=-1.12,95%CI[-1.47,0.77],P<0.00001);6 months endometrial thickness(MD=-1.90,95%CI[-2.38,-1.42],P<0.00001);endometrial thickness at 12 months(MD=-2.24,95%CI[-2.96,-1.52],P<0.00001);modified Kupperman score(MD=-10.45,95%CI[-19.10,-1.80],P=0.02);TCM syndrome score(SMD=-1.53,95%CI[-1.84,-1.22],P<0.00001);KPS score(MD=3.75,95%CI[2.81,4.68],P<0.00001);there was no significant difference in CA153,CEA,FSH and E2 between the two groups.Conclusion:After breast cancer surgery,endocrine therapy combined with Chinese herbal medicine can significantly lessen endometrial thickening and enhance patient quality of life.展开更多
目的评价γ-干扰素释放试验(interferon-gamma release assay,IGRA)在结核病专科门诊诊断儿童活动性结核病的效能。方法回顾性分析2019年1月至2021年12月就诊于西安市胸科医院结核病门诊的268例疑似结核病患儿为研究对象,采用area under...目的评价γ-干扰素释放试验(interferon-gamma release assay,IGRA)在结核病专科门诊诊断儿童活动性结核病的效能。方法回顾性分析2019年1月至2021年12月就诊于西安市胸科医院结核病门诊的268例疑似结核病患儿为研究对象,采用area under the ROC curve(AUC)评估在不同年龄阶段,有无卡痕,有无结核病密切接触史患儿中IGRA诊断活动性结核病的效能。结果在结核病专科门诊,IGRA诊断儿童活动性结核的敏感度、特异度、阴性预测值、阳性预测值、AUC分别为80.6%、63.6%、48.6%、88.5%、0.74。IGRA诊断儿童结核病AUC(95%CI值),在年龄≤5岁患儿中为0.71(0.62~0.80),低于6~14岁组的0.75(0.70~0.81),两组差异无统计学意义(Z=-0.848,P=0.40);在可见卡痕患儿中为0.72(0.67~0.78),与未见卡痕组的0.72(0.61~0.83)无统计学差异(Z=0.067,P=0.946);在有密接史组为0.83(0.75~0.91),高于无密接史组的0.72(0.66~0.77),两组差异有统计学意义(Z=2.336,P=0.020)。IGRA联合TST诊断儿童结核病AUC(95%CI值)为0.79(0.74~0.83),与TST单独诊断结核病AUC无统计学差异(Z=1.598,P=0.110)。结论在结核病专科门诊γ-干扰素释放试验诊断儿童活动性结核病准确度一般,在无结核病接触史患儿中其准确度较低,结核病专科门诊使用IGRA诊断儿童活动性结核病时需考虑其密切接触史情况。展开更多
从大豆基因组DNA中,通过PCR扩增获得Gm Gol S2-2启动子序列(Gm Gol S2-2P),长1 740 bp。预测分析结果显示,Gm Gol S2-2P中共含5种逆境相关顺式作用元件,包括2个水杨酸响应元件、1个茉莉酸甲酯响应元件、6个厌氧诱导元件、2个防御和胁迫...从大豆基因组DNA中,通过PCR扩增获得Gm Gol S2-2启动子序列(Gm Gol S2-2P),长1 740 bp。预测分析结果显示,Gm Gol S2-2P中共含5种逆境相关顺式作用元件,包括2个水杨酸响应元件、1个茉莉酸甲酯响应元件、6个厌氧诱导元件、2个防御和胁迫响应元件和1个MYB转录因子结合位点。构建植物表达载体p CAMBIA1301-GmGolS2-2P,通过叶盘法转化烟草。通过烟草基因组DNA PCR鉴定获得2棵T0代Gm Gol S2-2P转基因烟草植株。GUS组织化学染色和实时荧光定量RT-PCR结果表明,Gm Gol S2-2P在转基因烟草中能够激活GUS报告基因的表达,并且在干旱胁迫下Gm Gol S2-2P的启动活性增强。展开更多
基金State Administration of Traditional Chinese Medicine(No.ZyzB-2022-798)National Natural Science Foundation of China(No.82205222)Special Research Project of Beijing Municipal Health Commission on Capital Health Development(No.First edition 2022-4-2234)。
文摘Objective:To systematically evaluate the endometrial thickening and Chinese herbal medicine intervention effects during endocrine therapy following breast cancer surgery.Methods:Computerized searches were performed on CNKI,CBM,Wanfang,VIP,Pub Med,Embase,and Cochrane to gather randomized controlled trials(RCTs)of endometrial thickening combined with Chinese herbal medicine intervention during endocrine therapy after breast cancer surgery.The meta analysis is done using Rev Man 5.4,and the retrieval range is from the database's creation to January 2023.Results:There were 710 patients enrolled in a total of 12 RCTs.The results of Meta-analysis were as follows:combined with Chinese herbal medicine treatment for 3 months of endometrial thickness(MD=-1.12,95%CI[-1.47,0.77],P<0.00001);6 months endometrial thickness(MD=-1.90,95%CI[-2.38,-1.42],P<0.00001);endometrial thickness at 12 months(MD=-2.24,95%CI[-2.96,-1.52],P<0.00001);modified Kupperman score(MD=-10.45,95%CI[-19.10,-1.80],P=0.02);TCM syndrome score(SMD=-1.53,95%CI[-1.84,-1.22],P<0.00001);KPS score(MD=3.75,95%CI[2.81,4.68],P<0.00001);there was no significant difference in CA153,CEA,FSH and E2 between the two groups.Conclusion:After breast cancer surgery,endocrine therapy combined with Chinese herbal medicine can significantly lessen endometrial thickening and enhance patient quality of life.
文摘目的评价γ-干扰素释放试验(interferon-gamma release assay,IGRA)在结核病专科门诊诊断儿童活动性结核病的效能。方法回顾性分析2019年1月至2021年12月就诊于西安市胸科医院结核病门诊的268例疑似结核病患儿为研究对象,采用area under the ROC curve(AUC)评估在不同年龄阶段,有无卡痕,有无结核病密切接触史患儿中IGRA诊断活动性结核病的效能。结果在结核病专科门诊,IGRA诊断儿童活动性结核的敏感度、特异度、阴性预测值、阳性预测值、AUC分别为80.6%、63.6%、48.6%、88.5%、0.74。IGRA诊断儿童结核病AUC(95%CI值),在年龄≤5岁患儿中为0.71(0.62~0.80),低于6~14岁组的0.75(0.70~0.81),两组差异无统计学意义(Z=-0.848,P=0.40);在可见卡痕患儿中为0.72(0.67~0.78),与未见卡痕组的0.72(0.61~0.83)无统计学差异(Z=0.067,P=0.946);在有密接史组为0.83(0.75~0.91),高于无密接史组的0.72(0.66~0.77),两组差异有统计学意义(Z=2.336,P=0.020)。IGRA联合TST诊断儿童结核病AUC(95%CI值)为0.79(0.74~0.83),与TST单独诊断结核病AUC无统计学差异(Z=1.598,P=0.110)。结论在结核病专科门诊γ-干扰素释放试验诊断儿童活动性结核病准确度一般,在无结核病接触史患儿中其准确度较低,结核病专科门诊使用IGRA诊断儿童活动性结核病时需考虑其密切接触史情况。
文摘从大豆基因组DNA中,通过PCR扩增获得Gm Gol S2-2启动子序列(Gm Gol S2-2P),长1 740 bp。预测分析结果显示,Gm Gol S2-2P中共含5种逆境相关顺式作用元件,包括2个水杨酸响应元件、1个茉莉酸甲酯响应元件、6个厌氧诱导元件、2个防御和胁迫响应元件和1个MYB转录因子结合位点。构建植物表达载体p CAMBIA1301-GmGolS2-2P,通过叶盘法转化烟草。通过烟草基因组DNA PCR鉴定获得2棵T0代Gm Gol S2-2P转基因烟草植株。GUS组织化学染色和实时荧光定量RT-PCR结果表明,Gm Gol S2-2P在转基因烟草中能够激活GUS报告基因的表达,并且在干旱胁迫下Gm Gol S2-2P的启动活性增强。
