In this paper we define the pre-image topological pressure for a sequence of subadditive continuous functions on the compact metric space. And we also give a subadditive pre-image variational principle under a very we...In this paper we define the pre-image topological pressure for a sequence of subadditive continuous functions on the compact metric space. And we also give a subadditive pre-image variational principle under a very weak condition.展开更多
The present study aimed at the preparation of monoclonal antibody against the recombinant PthA-NLS and the isolation of the relative ScFv （single chain variable fragment） genes, providing the possibility to better u...The present study aimed at the preparation of monoclonal antibody against the recombinant PthA-NLS and the isolation of the relative ScFv （single chain variable fragment） genes, providing the possibility to better understand the pathogenesis mechanism via PthA, and developing proper construct for future experimentation to obtain citrus plants resistant to canker disease by transformation and plant antibody techniques. The recombinant polypeptide PthA-NLS was injected into Balb/c mice to produce monoclonal antibody. Total RNA was isolated from the hybridoma cell line 3D10H2 which secreted anti- PthA-NLS McAb, and the variable region genes were amplified with specific primers by RT-PCR and SOE-PCR （splicing by overlap extension）, and then the ScFv gene was isolated. The recombinant ScFv gene was cloned into pGEM-T and pET32a（＋） vector. The later plasmid was transferred into E. coli BL21 （DE3） and the expression of the recombinant protein was induced. Three cell lines producing monoclonal antibody against PthA-NLS were acquired and named 1C8H1, 2D12B6, and 3D8A10. The recombinant ScFv gene of about 750 bp was constructed. The sequencing results showed that the ScFv gene consists of a 360 bp heavy chain, a 342 bp light chain, and a 45 bp linker region. The recombinant fusion ScFv protein was expressed by IPTG induction, and a 44.5 kDa of recombinant fusion protein was obtained. In conclusion, we obtained three cell lines stably producing monoclonal antibody specifically bound to PthA-NLS, and the relative ScFv gene was constructed and successfully expressed in E. coli. These results may play an important role in further understanding the pathogenesis mechanism and in the development of possible citrus resistant to canker disease by genetic transformation and plant antibiobody.展开更多
Total RNA was isolated from the phloem of young shoots and retro-transcripted to cDNA by reverse transcriptasepolymerase chain reaction （RT-PCR） with specific primers; the amplified cDNA fragments were sequenced for...Total RNA was isolated from the phloem of young shoots and retro-transcripted to cDNA by reverse transcriptasepolymerase chain reaction （RT-PCR） with specific primers; the amplified cDNA fragments were sequenced for identification of subtypes. In this study, an effective RT-PCR technique was established for detecting citrus viroids, and three citrus viroids were recognized, namely, CEVd, CVd-II, and CVd-III. The latter two were identified for the first time in China. The sequences of CVd-Ⅱ were different from all the three subtypes reported in the GenBank and seemed to be a new subtype. CVd-Ⅲb detected in the present study could be further developed as a dwarfing agent.展开更多
We define the topological tail pressure and the conditional pressure for asymptotically sub-additive continuous potentials on topological dynamical systems and obtain a variational principle for the topological tail p...We define the topological tail pressure and the conditional pressure for asymptotically sub-additive continuous potentials on topological dynamical systems and obtain a variational principle for the topological tail pressure without any additional assumptions.展开更多
基金The first author is supported by a grant from Postdoctoral Science Research Program (0701049C) of Jiangsu Provincethe second author is partially supported by the NSF (10571086) of Chinathe National Basic Research Program-973 Program (2007CB814800) of China
文摘In this paper we define the pre-image topological pressure for a sequence of subadditive continuous functions on the compact metric space. And we also give a subadditive pre-image variational principle under a very weak condition.
基金supported by the Scientific and Technical Project of Hunan Province,China(04NK1005)
文摘The present study aimed at the preparation of monoclonal antibody against the recombinant PthA-NLS and the isolation of the relative ScFv （single chain variable fragment） genes, providing the possibility to better understand the pathogenesis mechanism via PthA, and developing proper construct for future experimentation to obtain citrus plants resistant to canker disease by transformation and plant antibody techniques. The recombinant polypeptide PthA-NLS was injected into Balb/c mice to produce monoclonal antibody. Total RNA was isolated from the hybridoma cell line 3D10H2 which secreted anti- PthA-NLS McAb, and the variable region genes were amplified with specific primers by RT-PCR and SOE-PCR （splicing by overlap extension）, and then the ScFv gene was isolated. The recombinant ScFv gene was cloned into pGEM-T and pET32a（＋） vector. The later plasmid was transferred into E. coli BL21 （DE3） and the expression of the recombinant protein was induced. Three cell lines producing monoclonal antibody against PthA-NLS were acquired and named 1C8H1, 2D12B6, and 3D8A10. The recombinant ScFv gene of about 750 bp was constructed. The sequencing results showed that the ScFv gene consists of a 360 bp heavy chain, a 342 bp light chain, and a 45 bp linker region. The recombinant fusion ScFv protein was expressed by IPTG induction, and a 44.5 kDa of recombinant fusion protein was obtained. In conclusion, we obtained three cell lines stably producing monoclonal antibody specifically bound to PthA-NLS, and the relative ScFv gene was constructed and successfully expressed in E. coli. These results may play an important role in further understanding the pathogenesis mechanism and in the development of possible citrus resistant to canker disease by genetic transformation and plant antibiobody.
基金supported by the Major Scientific and Technical Project in Hunan Province, China(04NK1005)
文摘Total RNA was isolated from the phloem of young shoots and retro-transcripted to cDNA by reverse transcriptasepolymerase chain reaction （RT-PCR） with specific primers; the amplified cDNA fragments were sequenced for identification of subtypes. In this study, an effective RT-PCR technique was established for detecting citrus viroids, and three citrus viroids were recognized, namely, CEVd, CVd-II, and CVd-III. The latter two were identified for the first time in China. The sequences of CVd-Ⅱ were different from all the three subtypes reported in the GenBank and seemed to be a new subtype. CVd-Ⅲb detected in the present study could be further developed as a dwarfing agent.
基金The NSF(11471114,11671208,11431012 and 11271191)of Chinathe National Basic Research Program(2013CB834100)of China(973 Program)
文摘We define the topological tail pressure and the conditional pressure for asymptotically sub-additive continuous potentials on topological dynamical systems and obtain a variational principle for the topological tail pressure without any additional assumptions.
