Innexin proteins are a class of transmembrane proteins existing in invertebrates and they have diverse biological functions. The innexin protein Sp-inx2 has been demonstrated to play roles in immune response and promo...Innexin proteins are a class of transmembrane proteins existing in invertebrates and they have diverse biological functions. The innexin protein Sp-inx2 has been demonstrated to play roles in immune response and promotion of cell apoptosis in the mud crab Scylla paramamosain . One novel innexin gene, named as Sp-inx3 was characterized from S. paramamosin in this study, with an open reading frame of 1 101 bp encoding 367 amino acid residues. Multiple sequence alignment revealed that the Sp-inx3 is highly homologous with innexin3 of Cancer boredis and Homorus americanus . Quantitative real-time PCR (qPCR) and the western blotting results revealed that Sp-inx3 gene was expressed predominantly in the eyestalk, brain, and thoracic ganglion mass in both female and male crabs. The immunohistochemistry assay (IHC) also showed the widespread and intense immunoreactivity of Sp-inx3 in the brain and thoracic ganglion mass. Sp-inx3 mRNA transcription profi les exhibited signifi cantly higher expression from the embryo1 to embryo4 period and low level of expression at the prehatching period and zoea I larva period of S . paramamosain . These results indicate that the Sp-inx3 may play an important role in the nervous system and early embryonic development of S . paramamosain.展开更多
为了探究曼氏无针乌贼胚胎发育和生长的适宜盐度,在水温(22.5±1.5)℃条件下,将曼氏无针乌贼受精卵置于试验组,盐度分别为5(S_(5))、10(S_(10))、20(S_(20))、30(S_(30))和40(S_(40)),并以本地海区盐度24(S_(24))为对照组,分析不同...为了探究曼氏无针乌贼胚胎发育和生长的适宜盐度,在水温(22.5±1.5)℃条件下,将曼氏无针乌贼受精卵置于试验组,盐度分别为5(S_(5))、10(S_(10))、20(S_(20))、30(S_(30))和40(S_(40)),并以本地海区盐度24(S_(24))为对照组,分析不同盐度对胚胎发育和子代的生长性能的影响。结果显示:曼氏无针乌贼胚胎发育时序分为17个阶段,试验组S_(30)完成整个胚胎发育的时间最短为19 d 3 h,且孵化率为(90.5±4.4)%,显著高于其他组(P<0.05),试验组S_(5)、S_(10)和S_(40)分别在胚胎发育至2细胞期、16细胞期和原肠晚期后停止发育;在盐度20~30区间,采用keys氏公式y=ax^(b)拟合1~30日龄的曼氏无针乌贼子代胴长(x)与体质量(y)的生长式型,b≈3呈等式生长;养殖30 d,试验组S_(30)的体质量绝对增加率、绝对增长率和体质量特定生长率分别为(0.33±0.12)g/d、(0.12±0.02)cm/d和(0.35±0.24)%/d,均显著大于对照组(P<0.05)。研究表明,盐度30较适宜曼氏无针乌贼胚胎生长发育。展开更多
基金Supported by the Fundamental Research Funds for the Central Universities(No.20720180100)the National Natural Science Foundation of China(NSFC)(Nos.U1205123,41676158)the Fujian Science and Technology Department(No.2014N2004)
文摘Innexin proteins are a class of transmembrane proteins existing in invertebrates and they have diverse biological functions. The innexin protein Sp-inx2 has been demonstrated to play roles in immune response and promotion of cell apoptosis in the mud crab Scylla paramamosain . One novel innexin gene, named as Sp-inx3 was characterized from S. paramamosin in this study, with an open reading frame of 1 101 bp encoding 367 amino acid residues. Multiple sequence alignment revealed that the Sp-inx3 is highly homologous with innexin3 of Cancer boredis and Homorus americanus . Quantitative real-time PCR (qPCR) and the western blotting results revealed that Sp-inx3 gene was expressed predominantly in the eyestalk, brain, and thoracic ganglion mass in both female and male crabs. The immunohistochemistry assay (IHC) also showed the widespread and intense immunoreactivity of Sp-inx3 in the brain and thoracic ganglion mass. Sp-inx3 mRNA transcription profi les exhibited signifi cantly higher expression from the embryo1 to embryo4 period and low level of expression at the prehatching period and zoea I larva period of S . paramamosain . These results indicate that the Sp-inx3 may play an important role in the nervous system and early embryonic development of S . paramamosain.
文摘为了探究曼氏无针乌贼胚胎发育和生长的适宜盐度,在水温(22.5±1.5)℃条件下,将曼氏无针乌贼受精卵置于试验组,盐度分别为5(S_(5))、10(S_(10))、20(S_(20))、30(S_(30))和40(S_(40)),并以本地海区盐度24(S_(24))为对照组,分析不同盐度对胚胎发育和子代的生长性能的影响。结果显示:曼氏无针乌贼胚胎发育时序分为17个阶段,试验组S_(30)完成整个胚胎发育的时间最短为19 d 3 h,且孵化率为(90.5±4.4)%,显著高于其他组(P<0.05),试验组S_(5)、S_(10)和S_(40)分别在胚胎发育至2细胞期、16细胞期和原肠晚期后停止发育;在盐度20~30区间,采用keys氏公式y=ax^(b)拟合1~30日龄的曼氏无针乌贼子代胴长(x)与体质量(y)的生长式型,b≈3呈等式生长;养殖30 d,试验组S_(30)的体质量绝对增加率、绝对增长率和体质量特定生长率分别为(0.33±0.12)g/d、(0.12±0.02)cm/d和(0.35±0.24)%/d,均显著大于对照组(P<0.05)。研究表明,盐度30较适宜曼氏无针乌贼胚胎生长发育。
