[ Objective] The aim of this study was to determine the effects of dietary phospholipids on Na+ - K+ - ATPase activity and cell mem- brane fatty acid composition in the gill of catfish (Pangasius sutchi). [Method]...[ Objective] The aim of this study was to determine the effects of dietary phospholipids on Na+ - K+ - ATPase activity and cell mem- brane fatty acid composition in the gill of catfish (Pangasius sutchi). [Method] 900 healthy catfish (1.4 ±0.08 g of average weight) were randomly divided into five groups. Dietary phospholipids level of group PL0 was 0%, and 1%, 2%, 3%, 4% for group PL1, PL2, PL3, PL4, respectively. The experiment lasted for 56 days. [Result] The results showed that the Na+ -K+ -ATPase activity in gill decreased gradually with the increase of the dietary phospholipids level ( P 〈0.05). Cell membrane fatty acid composition was affected significantly by dietary phospholipids. The content of saturated fatty acids and monounsaturated fatty acids of group PLO was the highest ( P 〈 0.05), however, it had the lowest content of highlyunsatu- rated fatty acids ( P 〈 0.05). The content of polyunsaturated fatty acids of group 3 was higher than group 0 and group 1 ( P 〈 0.05). E Conclusion] The results indicated that there is obvious compensation effects on Na+ -K+ -ATPase activity with phospholipids content changes in gills of catfish ( Pangasius sutchi) to maintain stable physiological level and normal basal metabolism and the increase of HUFA and ∑PUFA is necessary for gill cell membrane to better maintain and perform normal physiological function.展开更多
基金supported by Guangxi Natural Science Foundation(2012GXNSFBA053053)The"Twelfth Five-Year"National Science and Technology Support Program ( 2012BAD25B04)Guangxi University"Student Innovation Training Program"(201152)
文摘[ Objective] The aim of this study was to determine the effects of dietary phospholipids on Na+ - K+ - ATPase activity and cell mem- brane fatty acid composition in the gill of catfish (Pangasius sutchi). [Method] 900 healthy catfish (1.4 ±0.08 g of average weight) were randomly divided into five groups. Dietary phospholipids level of group PL0 was 0%, and 1%, 2%, 3%, 4% for group PL1, PL2, PL3, PL4, respectively. The experiment lasted for 56 days. [Result] The results showed that the Na+ -K+ -ATPase activity in gill decreased gradually with the increase of the dietary phospholipids level ( P 〈0.05). Cell membrane fatty acid composition was affected significantly by dietary phospholipids. The content of saturated fatty acids and monounsaturated fatty acids of group PLO was the highest ( P 〈 0.05), however, it had the lowest content of highlyunsatu- rated fatty acids ( P 〈 0.05). The content of polyunsaturated fatty acids of group 3 was higher than group 0 and group 1 ( P 〈 0.05). E Conclusion] The results indicated that there is obvious compensation effects on Na+ -K+ -ATPase activity with phospholipids content changes in gills of catfish ( Pangasius sutchi) to maintain stable physiological level and normal basal metabolism and the increase of HUFA and ∑PUFA is necessary for gill cell membrane to better maintain and perform normal physiological function.