Mechanisms of imperatorin on regulating Pglycoprotein in blood-brain barrier based on network pharmacology and in vitro experiment

OBJECTIVE To explore mecha⁃nisms of imperatorin on regulating P-glycoprotein(P-gp)in blood-brain barrier(BBB)based on net⁃work pharmacology combined with in vitro experi⁃ment.METHODS Drug targets were predicted using the Pharmapper and Swiss targets data⁃bases;disease targets were obtained through the Genecards database;intersections between drugs and disease targets were screened by Cytoscape software;the obtained core targets were used to construct protein-protein interaction(PPI)network,gene ontology(GO)functions,and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis.The effects of imperatorin(20,50,100μmol·L^(-1))on P-gp activity were monitored in hCMEC/D3 in vitro BBB model,and the effects of imperatorin on the expression of target proteins were verified using Western blot method.RESULTS 55 drug targets and 3102 disease targets were obtained from the network pharmacology screening,and 37 core targets were obtained after the combination.Enrichment analysis showed that core targets were closely related to chemical synaptic trans⁃mission regulation,neurotransmitter receptor activity,protein kinase regulation activity,G proteincoupled receptor signaling pathway,neural active ligand receptor interaction pathway,PI3K-Akt sig⁃naling pathway,VEGF signaling pathway,etc..In vitro experimental validation suggested that all tested concentration groups of imperatorin signifi⁃cantly reduced the activity and expression of P-gp,which were achieved by significantly downregu⁃lating the phosphorylation levels of PI3K and Akt,and repressing the expression of VEGFR2 pro⁃tein.CONCLUSION Network pharmacology was used to predict the core targets and signaling pathways of imperatorin on regulating P-gp in BBB and relevant validation was conducted through in vitro experiments,providing a refer⁃ence basis for further exploration of the mecha⁃nisms of imperatorin on regulating P-gp in BBB.

基于服务架构的OpenFOAM求解客户端设计

针对OpenFOAM解算软件缺乏GUI功能用户交互体验性差问题,研究设计了一个OpenFOAM求解客户端软件。设计了基于服务的“云+端”计算架构,设计开发了一个OpenFOAM求解器服务,GUI客户端远程调用访问求解器服务,并集成可视化服务实现了数据可视化分析功能,实现了云端协同的求解计算功能;研究设计了基于界面模板的动态GUI界面生成方法,实现了客户端软件界面用户自定义配置功能。该软件支持基于服务的功能扩展集成,支持用户界面自定义、求解器用户设置界面自定义功能,用户界面友好,可扩展性强。通过实际的算例进行解算,验证表明了客户端软件能够通过远程调用云端求解服务、可视化服务实现完整的求解计算流程。

面向云计算环境的CFD数据可视化分析服务

针对目前CFD(Computational Fluid Dynamics)软件数据可视化分析所需计算量大且操作不灵活的问题,面向CFD数据可视化分析需求,实现了一个CFD数据可视化分析服务原型软件。在该原型软件中,研究了自适应客户端的服务自适应QoS(Quality of Service)优化技术,降低不同客户端计算环境对服务质量的影响;研究设计了基于消息中间件的服务集成接口,提高了服务使用的灵活性。面向云计算环境的CFD数据可视化分析服务发挥了云端服务器的性能优势,应用了“云+端”的开发新模式,具备灵活性和可扩展性。

基于自适应牵制控制的时变时滞复杂网络同步

针对一类节点含时变时滞的复杂网络模型的同步问题,提出一种简单有效的自适应牵制控制方法。设计了一个自适应控制器,对复杂网络的部分节点实施牵制控制;构造适当的Lyapunov函数,给出网络和其孤立节点达到全局指数渐近同步的充分条件;最后通过数值仿真实验验证了文中方法的有效性。