Transcription factor NAC102 plays an important role in the abiotic stress responses of plants.In this study,the promoter sequence of 3000 bp located in the upstream of the BjNAC102 gene was cloned from Brassica juncea...Transcription factor NAC102 plays an important role in the abiotic stress responses of plants.In this study,the promoter sequence of 3000 bp located in the upstream of the BjNAC102 gene was cloned from Brassica juncea‘Sichuan Yellow Seed’by using the homologous cloning method.The expression vector of the GUS gene driven by the BjNAC102 promoter was constructed by seamless cloning technology.The results showed that the sequence of the promoter of the BjNAC102 gene contained many cis-acting elements involved in light responsiveness,gibberellinresponsive element,and auxin-responsive element.It was speculated that BjNAC102 played an important role in the abiotic stress response in Brassica juncea.The expression vector of the promoter of the BjNAC102 gene was constructed,which layed a foundation for further studies of the expression pattern of the BjNAC102 gene in Brassica juncea.展开更多
目的分析Roux-en-Y式胃癌根治术联合胃旁路手术治疗进展期胃癌的疗效及对患者肠道屏障功能及并发症的影响。方法回顾性研究,选取2016年8月至2019年1月攀枝花市中心医院收治的进展期胃癌80例患者,按手术方式将其分为Roux-en-Y式胃癌根治...目的分析Roux-en-Y式胃癌根治术联合胃旁路手术治疗进展期胃癌的疗效及对患者肠道屏障功能及并发症的影响。方法回顾性研究,选取2016年8月至2019年1月攀枝花市中心医院收治的进展期胃癌80例患者,按手术方式将其分为Roux-en-Y式胃癌根治术组(传统组,n=38)、Roux-en-Y式胃癌根治术联合胃旁路手术组(联合组,n=42)。统计两组手术时间、术中出血量、肛门排气时间、拔管时间、下床活动时间、住院时间,比较两组手术前后肠道屏障功能指标[血二胺氧化酶(DAO)、内毒素脂多糖(LPS)、肿瘤坏死因子-α(TNF-α)、尿乳果糖/甘露醇(L/M)比值]的变化,记录患者随访6个月进食量、进食次数、体重、血红蛋白、白蛋白等营养指标及胃肠道症状(GSPS)评分的变化,统计手术并发症发生率。结果两组手术时间、术中出血量、下床活动时间、拔管时间、住院时间等资料比较,差异均无统计学意义(P>0.05)。术后12 d,两组DAO、LPS、L/M上升,TNF-α降低,联合组术后12 d LPS、TNF-α、L/M为(0.86±0.26)EU/mL、(5.13±2.93)mg/L、(0.06±0.01),均低于传统组[(1.36±0.34)EU/mL、(7.12±2.69)mg/L、(0.08±0.03)],差异均有统计学意义(P<0.05)。术后6个月,两组进食次数减少、血红蛋白上升、白蛋白及GSPS评分降低,联合组血红蛋白为(118.01±16.85)g/L,高于传统组[(108.67±15.26)g/L],GSPS评分为(3.39±0.27)分,低于传统组[(5.41±0.52)分],差异均有统计学意义(P<0.05)。两组手术近远期并发症发生率比较,差异无统计学意义(P>0.05)。结论Roux-en-Y式胃癌根治术联合胃旁路手术治疗进展期胃癌疗效肯定,存在肠道屏障功能保护效应,可改善患者术后营养状态,不会增加手术并发症发生风险,临床应用可行性强。展开更多
基金Supported by Natural Science Foundation of Hunan Province(2023JJ50083,2023JJ50084)Excellent Youth Project of Hunan Provincial Department of Education(22B0844)Hunan Provincial Graduate Research Innovation Project(CX20231274)。
文摘Transcription factor NAC102 plays an important role in the abiotic stress responses of plants.In this study,the promoter sequence of 3000 bp located in the upstream of the BjNAC102 gene was cloned from Brassica juncea‘Sichuan Yellow Seed’by using the homologous cloning method.The expression vector of the GUS gene driven by the BjNAC102 promoter was constructed by seamless cloning technology.The results showed that the sequence of the promoter of the BjNAC102 gene contained many cis-acting elements involved in light responsiveness,gibberellinresponsive element,and auxin-responsive element.It was speculated that BjNAC102 played an important role in the abiotic stress response in Brassica juncea.The expression vector of the promoter of the BjNAC102 gene was constructed,which layed a foundation for further studies of the expression pattern of the BjNAC102 gene in Brassica juncea.
文摘目的分析Roux-en-Y式胃癌根治术联合胃旁路手术治疗进展期胃癌的疗效及对患者肠道屏障功能及并发症的影响。方法回顾性研究,选取2016年8月至2019年1月攀枝花市中心医院收治的进展期胃癌80例患者,按手术方式将其分为Roux-en-Y式胃癌根治术组(传统组,n=38)、Roux-en-Y式胃癌根治术联合胃旁路手术组(联合组,n=42)。统计两组手术时间、术中出血量、肛门排气时间、拔管时间、下床活动时间、住院时间,比较两组手术前后肠道屏障功能指标[血二胺氧化酶(DAO)、内毒素脂多糖(LPS)、肿瘤坏死因子-α(TNF-α)、尿乳果糖/甘露醇(L/M)比值]的变化,记录患者随访6个月进食量、进食次数、体重、血红蛋白、白蛋白等营养指标及胃肠道症状(GSPS)评分的变化,统计手术并发症发生率。结果两组手术时间、术中出血量、下床活动时间、拔管时间、住院时间等资料比较,差异均无统计学意义(P>0.05)。术后12 d,两组DAO、LPS、L/M上升,TNF-α降低,联合组术后12 d LPS、TNF-α、L/M为(0.86±0.26)EU/mL、(5.13±2.93)mg/L、(0.06±0.01),均低于传统组[(1.36±0.34)EU/mL、(7.12±2.69)mg/L、(0.08±0.03)],差异均有统计学意义(P<0.05)。术后6个月,两组进食次数减少、血红蛋白上升、白蛋白及GSPS评分降低,联合组血红蛋白为(118.01±16.85)g/L,高于传统组[(108.67±15.26)g/L],GSPS评分为(3.39±0.27)分,低于传统组[(5.41±0.52)分],差异均有统计学意义(P<0.05)。两组手术近远期并发症发生率比较,差异无统计学意义(P>0.05)。结论Roux-en-Y式胃癌根治术联合胃旁路手术治疗进展期胃癌疗效肯定,存在肠道屏障功能保护效应,可改善患者术后营养状态,不会增加手术并发症发生风险,临床应用可行性强。
