Owing to the limitation of a large genome size(~13 Gb),the genetic and gene mapping studies on faba bean(Vicia faba L.)are lagging far behind those for other legumes.In this study,we selected three purified faba bean ...Owing to the limitation of a large genome size(~13 Gb),the genetic and gene mapping studies on faba bean(Vicia faba L.)are lagging far behind those for other legumes.In this study,we selected three purified faba bean lines(Yundou 8137,H0003712,and H000572)as parents and constructed two F2 populations.These two F2 populations,namely 167 F2 plants in Pop1(Yundou 8137×H0003712)and 204 F2 plants in Pop2(H000572×Yundou 8137),were genotyped using a targeted next-generation sequencing(TNGS)genotyping platform,and two high-density single nucleotide polymorphisms(SNP)genetic linkage maps of faba bean were constructed.The map constructed from Pop1 contained 5103 SNPs with a length of 1333.31 cM and an average marker density of 0.26 cM.The map constructed from Pop2 contained 1904 SNPs with a greater length of 1610.61 cM.In these two F2 populations,QTL mapping identified 98 QTLs for 14 agronomic traits related to the flowers,pods,plant types and grains.The two maps were then merged into an integrated genetic linkage map containing 6895 SNPs,with a length of 3324.48 cM.These results not only lay the foundation for fine mapping and map-based cloning of related genes,but can also accelerate the molecular marker-assisted breeding of faba bean.展开更多
黄河流域是我国重要的生态安全屏障和经济活动区域,其生态环境直接关系到我国西北、华北地区的生态安全和可持续发展.为深入了解黄河流域生态状况研究的发展趋势、研究现状及研究热点,本文基于Web of Science核心合集数据库和中国期刊...黄河流域是我国重要的生态安全屏障和经济活动区域,其生态环境直接关系到我国西北、华北地区的生态安全和可持续发展.为深入了解黄河流域生态状况研究的发展趋势、研究现状及研究热点,本文基于Web of Science核心合集数据库和中国期刊全文数据库CNKI,检索了1981-2022年有关“黄河流域生态状况”的中英文文献,并对9400篇文献进行计量分析,据此整理出该研究领域的发展趋势和研究热点,对不同研究区域在研究内容上的差异进行分析.结果表明:黄河流域生态状况研究正处于高速发展阶段,热点研究内容包括生态环境、水资源和水土保持,热点研究对象包括生态系统、植被和水生生物,研究区域涵盖黄河全流域,影响黄河流域生态状况的重点研究因素包括人类活动、环境因子和时空因素.此外,不同研究区域所涉及的影响因素和研究对象具有差异性:从影响因素看,在黄河上、中、下游地区主要关注人类活动的影响,而在黄河三角洲和黄河河口地区重点关注水质和重金属等环境因子,在黄河全流域和黄河源区更注重时空因素的影响;从研究对象看,在黄河源区主要关注草地生态系统,而在黄河上、中、下游以及黄河三角洲地区主要集中在生态系统和植被方面,黄河河口的研究则更加侧重水生生物群落.根据以上研究结果,未来关于黄河流域及其特定河段的研究需要在选择研究对象和影响因素时考虑研究区域的特点,以确保研究的科学性;同时,统筹发展要求科研工作者不断更新研究方法和评价方法,将环境DNA、遥感等技术手段和综合的环境评价指标应用到流域生态状况评价研究中.展开更多
基金supported by the National Key R&D Program of China(2019YFD1001300 and 2019YFD1001303)the Construction of Molecular Database of Faba Bean and Pea and Identification of Maize Germplasm Project,Ministry of Agriculture and Rural Affairs,China(19200030)+3 种基金the Yunnan Key R&D Program,China(202202AE090003)the earmarked fund for China Agriculture Research System(CARS-08)the Crop Germplasm Resources Protection(2130135)the Major Agricultural Science and Technology Program of Chinese Academy of Agricultural Sciences(CAAS-XTCX20190025)。
文摘Owing to the limitation of a large genome size(~13 Gb),the genetic and gene mapping studies on faba bean(Vicia faba L.)are lagging far behind those for other legumes.In this study,we selected three purified faba bean lines(Yundou 8137,H0003712,and H000572)as parents and constructed two F2 populations.These two F2 populations,namely 167 F2 plants in Pop1(Yundou 8137×H0003712)and 204 F2 plants in Pop2(H000572×Yundou 8137),were genotyped using a targeted next-generation sequencing(TNGS)genotyping platform,and two high-density single nucleotide polymorphisms(SNP)genetic linkage maps of faba bean were constructed.The map constructed from Pop1 contained 5103 SNPs with a length of 1333.31 cM and an average marker density of 0.26 cM.The map constructed from Pop2 contained 1904 SNPs with a greater length of 1610.61 cM.In these two F2 populations,QTL mapping identified 98 QTLs for 14 agronomic traits related to the flowers,pods,plant types and grains.The two maps were then merged into an integrated genetic linkage map containing 6895 SNPs,with a length of 3324.48 cM.These results not only lay the foundation for fine mapping and map-based cloning of related genes,but can also accelerate the molecular marker-assisted breeding of faba bean.
文摘黄河流域是我国重要的生态安全屏障和经济活动区域,其生态环境直接关系到我国西北、华北地区的生态安全和可持续发展.为深入了解黄河流域生态状况研究的发展趋势、研究现状及研究热点,本文基于Web of Science核心合集数据库和中国期刊全文数据库CNKI,检索了1981-2022年有关“黄河流域生态状况”的中英文文献,并对9400篇文献进行计量分析,据此整理出该研究领域的发展趋势和研究热点,对不同研究区域在研究内容上的差异进行分析.结果表明:黄河流域生态状况研究正处于高速发展阶段,热点研究内容包括生态环境、水资源和水土保持,热点研究对象包括生态系统、植被和水生生物,研究区域涵盖黄河全流域,影响黄河流域生态状况的重点研究因素包括人类活动、环境因子和时空因素.此外,不同研究区域所涉及的影响因素和研究对象具有差异性:从影响因素看,在黄河上、中、下游地区主要关注人类活动的影响,而在黄河三角洲和黄河河口地区重点关注水质和重金属等环境因子,在黄河全流域和黄河源区更注重时空因素的影响;从研究对象看,在黄河源区主要关注草地生态系统,而在黄河上、中、下游以及黄河三角洲地区主要集中在生态系统和植被方面,黄河河口的研究则更加侧重水生生物群落.根据以上研究结果,未来关于黄河流域及其特定河段的研究需要在选择研究对象和影响因素时考虑研究区域的特点,以确保研究的科学性;同时,统筹发展要求科研工作者不断更新研究方法和评价方法,将环境DNA、遥感等技术手段和综合的环境评价指标应用到流域生态状况评价研究中.
