Objective:To explore the related mechanism of miR-34a affecting epilepsy.Methods:MicroRNA microarray chip and RT-PCR were used to analyze the expression of miR-34a in epileptic rats,and observe the relationship betwee...Objective:To explore the related mechanism of miR-34a affecting epilepsy.Methods:MicroRNA microarray chip and RT-PCR were used to analyze the expression of miR-34a in epileptic rats,and observe the relationship between miR-34a and seizure frequency and EEG.Predicted the target gene of miR-34a,used The dual-luciferase reporter gene assay was used to observe its binding to the target gene of miR-34a,the effect of overexpression and inhibition of miR-34a on the target gene,the effects of overexpression and inhibition of target genes on downstream factors.Results:Microarray chip and RT-PCR detection showed that miR-34a was highly expressed in epilepsy(P<0.05),and miR-34a was positively correlated with seizure frequency(R=0.783,P=0.003),and inhibition of miR-34a inhibited the seizure frequency of epilepsy(P<0.05).The online databases TargetScan and miRDB show that miR-34a and BCL-2 have a predicted binding region,and the dual-luciferase reporter gene experiment proves that the two have a binding relationship.When miR-34a was overexpressed,BCL-2 expression was down-regulated,and conversely,when miR-34a was inhibited,BCL-2 expression was up-regulated.NLRP1 and downstream related factors Caspase-3,Caspase-1,IL-1βand IL-18 were highly expressed in the brain tissue of epileptic rats(P<0.05).When BCL-2 was inhibited,the expression levels of NLRP1 and downstream related factors were increased,and when BCL-2 was inhibited,the result was the opposite.Conclusion:The highly expressed miR-34a promotes epilepsy by inhibiting BCL-2 and activating the NLRP1 inflammasome.展开更多
基金Guangxi Natural Science Foundation(No.2020GXNSFAA238007)Guangxi High-level Innovation Team and Outstanding Scholars Program[Guangxi Higher Education Talents(2020)No.6]+2 种基金Academic Team Building Project of the First Affiliated Hospital of Guangxi TCM University(NO:Courtyard Word[2018]No.146)Guangxi Clinical Research Center of Traditional Chinese Medicine encephalopathy(NO.AD20238028)Guangxi Traditional Chinese Medicine Key Discipline Construction Project(No.GZXK-Z-20-13)。
文摘Objective:To explore the related mechanism of miR-34a affecting epilepsy.Methods:MicroRNA microarray chip and RT-PCR were used to analyze the expression of miR-34a in epileptic rats,and observe the relationship between miR-34a and seizure frequency and EEG.Predicted the target gene of miR-34a,used The dual-luciferase reporter gene assay was used to observe its binding to the target gene of miR-34a,the effect of overexpression and inhibition of miR-34a on the target gene,the effects of overexpression and inhibition of target genes on downstream factors.Results:Microarray chip and RT-PCR detection showed that miR-34a was highly expressed in epilepsy(P<0.05),and miR-34a was positively correlated with seizure frequency(R=0.783,P=0.003),and inhibition of miR-34a inhibited the seizure frequency of epilepsy(P<0.05).The online databases TargetScan and miRDB show that miR-34a and BCL-2 have a predicted binding region,and the dual-luciferase reporter gene experiment proves that the two have a binding relationship.When miR-34a was overexpressed,BCL-2 expression was down-regulated,and conversely,when miR-34a was inhibited,BCL-2 expression was up-regulated.NLRP1 and downstream related factors Caspase-3,Caspase-1,IL-1βand IL-18 were highly expressed in the brain tissue of epileptic rats(P<0.05).When BCL-2 was inhibited,the expression levels of NLRP1 and downstream related factors were increased,and when BCL-2 was inhibited,the result was the opposite.Conclusion:The highly expressed miR-34a promotes epilepsy by inhibiting BCL-2 and activating the NLRP1 inflammasome.
