Taking into account that fuzzy ontology mapping has wide application and cannot be dealt with in many fields at present,a Chinese fuzzy ontology model and a method for Chinese fuzzy ontology mapping are proposed.The m...Taking into account that fuzzy ontology mapping has wide application and cannot be dealt with in many fields at present,a Chinese fuzzy ontology model and a method for Chinese fuzzy ontology mapping are proposed.The mapping discovery between two ontologies is achieved by computing the similarity between the concepts of two ontologies.Every concept consists of four features of concept name,property,instance and structure.First,the algorithms of calculating four individual similarities corresponding to the four features are given.Secondly,the similarity vectors consisting of four weighted individual similarities are built,and the weights are the linear function of harmony and reliability.The similarity vector is used to represent the similarity relation between two concepts which belong to different fuzzy ontolgoies.Lastly,Support Vector Machine(SVM) is used to get the mapping concept pairs by the similarity vectors.Experiment results are satisfactory.展开更多
[ Objective] To establish a Taqman real-time PCR for detection of Salmonella in pet food. [Method] A pair of primers and a probe were designed based on published nucleotide sequence of invA gene encoding the invasion ...[ Objective] To establish a Taqman real-time PCR for detection of Salmonella in pet food. [Method] A pair of primers and a probe were designed based on published nucleotide sequence of invA gene encoding the invasion protein of Salmonella enterica. [ Result] The assay detects Salmonella specifically. The detection limit of the real-time PCR was 17 CFU/test （25 uL/test） for the positive strain. This method was effective to detect artificially contaminated pet food. [ Conclusion] The results showed that Taqman PCR assay was rapid and accurate for detection of Salmonella from infected pet food.展开更多
IM To study the p53 gene mutation and its relationship to aflatoxin B1 exposure in hepatocellular carcinoma (HCC).METHODS Restriction fragment length polymorphism analysis method was used in 62 HCC samples, and DNA...IM To study the p53 gene mutation and its relationship to aflatoxin B1 exposure in hepatocellular carcinoma (HCC).METHODS Restriction fragment length polymorphism analysis method was used in 62 HCC samples, and DNA direct sequencing in another 45 HCC samples.RESULTS In HCC and AFB1 high and lowrisk areas, 36/52 (69%) and 2/10 (20%) cases were found losing the HaeⅢ allele respectively, suggesting one of the base G mutation at the p53 gene codon 249. Similar results appeared in DNA direct sequencing, 20/35 (57%) and 1/10 (10%) respectively mutated at the codon 249 third base G to C transversion.CONCLUSION In HCC after AFB1 exposure, mutation of p53 gene is fixed at codon 249 third base and take the form of G to T transversion. This is a definite marker of mutation which is induced by AFB1 mutagen. It is applicable for molecular epidemiologic survey of the sufferers of AFB1 among HCC cases and for discovering more unknown natural AFB1 contaminated areas..展开更多
The fields of structural geology and tectonics have witnessed great progress over the last decade and are poised for further expansion in the future. One of the significant breakthroughs is the establishment of the &#...The fields of structural geology and tectonics have witnessed great progress over the last decade and are poised for further expansion in the future. One of the significant breakthroughs is the establishment of the 'Beyond Plate Tectonics Theory' where a combination of conceptual models and numerical modeling on plume tectonics and plate tectonics has enabled new insights into the structural and tectonic architecture and processes in the deep interior and deep sea. This paper synthesizes developments of structural geology and tectonics from a macroscopic perspective in deep interior and deep sea. Four key techniques are also reviewed: satellite altimetry for surface structures in deep-sea multi-beam sea-floor mapping; tomography for tectonics of the deep interior; diverse modeling approaches and software for unfolding dynamic evolution; and techniques for HT/HP experiments on material rheology and in situ component measurements.展开更多
基金supported by the Natural Science Foundation of Beijing City under Grant No.4123094the Science and Technology Project of Beijing Municipal Commission of Education under Grants No.KM201110028020,No.KM201010028019+1 种基金the National Nature Science Foundation under Grants No.61100205,No.60873001,No.60863011,No.61175068the Fundamental Research Funds for the Central Universities under Grant No.2009RC0212
文摘Taking into account that fuzzy ontology mapping has wide application and cannot be dealt with in many fields at present,a Chinese fuzzy ontology model and a method for Chinese fuzzy ontology mapping are proposed.The mapping discovery between two ontologies is achieved by computing the similarity between the concepts of two ontologies.Every concept consists of four features of concept name,property,instance and structure.First,the algorithms of calculating four individual similarities corresponding to the four features are given.Secondly,the similarity vectors consisting of four weighted individual similarities are built,and the weights are the linear function of harmony and reliability.The similarity vector is used to represent the similarity relation between two concepts which belong to different fuzzy ontolgoies.Lastly,Support Vector Machine(SVM) is used to get the mapping concept pairs by the similarity vectors.Experiment results are satisfactory.
基金Supported by the Project of General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China( 201110034)
文摘[ Objective] To establish a Taqman real-time PCR for detection of Salmonella in pet food. [Method] A pair of primers and a probe were designed based on published nucleotide sequence of invA gene encoding the invasion protein of Salmonella enterica. [ Result] The assay detects Salmonella specifically. The detection limit of the real-time PCR was 17 CFU/test （25 uL/test） for the positive strain. This method was effective to detect artificially contaminated pet food. [ Conclusion] The results showed that Taqman PCR assay was rapid and accurate for detection of Salmonella from infected pet food.
文摘IM To study the p53 gene mutation and its relationship to aflatoxin B1 exposure in hepatocellular carcinoma (HCC).METHODS Restriction fragment length polymorphism analysis method was used in 62 HCC samples, and DNA direct sequencing in another 45 HCC samples.RESULTS In HCC and AFB1 high and lowrisk areas, 36/52 (69%) and 2/10 (20%) cases were found losing the HaeⅢ allele respectively, suggesting one of the base G mutation at the p53 gene codon 249. Similar results appeared in DNA direct sequencing, 20/35 (57%) and 1/10 (10%) respectively mutated at the codon 249 third base G to C transversion.CONCLUSION In HCC after AFB1 exposure, mutation of p53 gene is fixed at codon 249 third base and take the form of G to T transversion. This is a definite marker of mutation which is induced by AFB1 mutagen. It is applicable for molecular epidemiologic survey of the sufferers of AFB1 among HCC cases and for discovering more unknown natural AFB1 contaminated areas..
基金Funding Sources:Marine 863 Project (No.2009AA093401)Projects of the National Natural Science Foundation of China (Nos.41072152,90814011 and 41190072)
文摘The fields of structural geology and tectonics have witnessed great progress over the last decade and are poised for further expansion in the future. One of the significant breakthroughs is the establishment of the 'Beyond Plate Tectonics Theory' where a combination of conceptual models and numerical modeling on plume tectonics and plate tectonics has enabled new insights into the structural and tectonic architecture and processes in the deep interior and deep sea. This paper synthesizes developments of structural geology and tectonics from a macroscopic perspective in deep interior and deep sea. Four key techniques are also reviewed: satellite altimetry for surface structures in deep-sea multi-beam sea-floor mapping; tomography for tectonics of the deep interior; diverse modeling approaches and software for unfolding dynamic evolution; and techniques for HT/HP experiments on material rheology and in situ component measurements.
