The network of microbial electron transfer can establish a syntrophic association of microbes by connecting interspecies metabolisms, and a variety of redox-active shuttles in environment have been proved to accelerat...The network of microbial electron transfer can establish a syntrophic association of microbes by connecting interspecies metabolisms, and a variety of redox-active shuttles in environment have been proved to accelerate the electron flow in a microbial community. Using humic substances as models, we investigated how different redox-active shuttles with different electrochemical properties influence interspecies electron transfer, and affect the shift of microbial communities. The co-culture of two species was constructed with supplements of humics, and the electron transfer between these two strains was found to be linked by humic acid with a wider window of redox potential and multi-peaks of redox reactions. Based on the shift of microbial composition, the humic substances with a wide potential window and multi-peaks of redox reactions for accepting and donating electrons could increase the biodiversity(Chao 1 and phylogenetic diversity) with a large extent. The mechanism by which redox-active shuttles mediate the microbial electron transfer network could facilitate our understanding of syntrophic interactions between microbes.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.41471260,21777155)
文摘The network of microbial electron transfer can establish a syntrophic association of microbes by connecting interspecies metabolisms, and a variety of redox-active shuttles in environment have been proved to accelerate the electron flow in a microbial community. Using humic substances as models, we investigated how different redox-active shuttles with different electrochemical properties influence interspecies electron transfer, and affect the shift of microbial communities. The co-culture of two species was constructed with supplements of humics, and the electron transfer between these two strains was found to be linked by humic acid with a wider window of redox potential and multi-peaks of redox reactions. Based on the shift of microbial composition, the humic substances with a wide potential window and multi-peaks of redox reactions for accepting and donating electrons could increase the biodiversity(Chao 1 and phylogenetic diversity) with a large extent. The mechanism by which redox-active shuttles mediate the microbial electron transfer network could facilitate our understanding of syntrophic interactions between microbes.
