Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device....Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device.Unlike the conventional culture dish method,two different epidermal cells are successfully adhered to the front and back sides of the patch,which produces a three-dimensional nanofibrous scaffold similar to a natural extracellular matrix before the patch was cultured in the skin-on-a-chip device to mimic the physiological conditions of human skin.As expected,the differentiated hiPSCs show the expression of keratinocyte-and fibroblast-specific proteins on the patch,and the layering is found between these two kinds of cells,indicating that this approach creates a powerful in vitro system for modeling skin development and diseases.展开更多
为了建立一种快速、准确地检测布氏杆菌S2疫苗株的环介导等温扩增(LAMP)方法,将S2基因组与牛种、羊种野毒株基因组比较,筛选出S2疫苗特有基因trbJ作为检测靶标序列,设计LAMP特异性引物,在对该反应组分、反应条件进行单因素优化的同时,...为了建立一种快速、准确地检测布氏杆菌S2疫苗株的环介导等温扩增(LAMP)方法,将S2基因组与牛种、羊种野毒株基因组比较,筛选出S2疫苗特有基因trbJ作为检测靶标序列,设计LAMP特异性引物,在对该反应组分、反应条件进行单因素优化的同时,还对该引物特异性、灵敏度、重复性进行测试。结果显示,该方法在65℃等温条件下扩增,反应结束后加入SYBR Green I荧光染料,即可通过肉眼直接观察结果。该方法灵敏性、特异性、重复性良好,能准确鉴别出S2疫苗,与对照菌株无交叉反应。与普通PCR技术相比,LAMP检测灵敏度高100倍,最低可检测出6.89 pg/μL的DNA模板,并且不同批次试剂间的重复性良好。本试验成功建立了一种快速、准确、可视化地检测S2疫苗的LAMP方法。展开更多
基金by the National Natural Science Foundation of China(Nos.31600801 and 11804121)the Natural Science Foundation of Hubei(No.2019CFB558)the Central Government Guides the Special Fund Project of Local Scientific and Technological Development(No.[2016]109)。
文摘Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device.Unlike the conventional culture dish method,two different epidermal cells are successfully adhered to the front and back sides of the patch,which produces a three-dimensional nanofibrous scaffold similar to a natural extracellular matrix before the patch was cultured in the skin-on-a-chip device to mimic the physiological conditions of human skin.As expected,the differentiated hiPSCs show the expression of keratinocyte-and fibroblast-specific proteins on the patch,and the layering is found between these two kinds of cells,indicating that this approach creates a powerful in vitro system for modeling skin development and diseases.
文摘为了建立一种快速、准确地检测布氏杆菌S2疫苗株的环介导等温扩增(LAMP)方法,将S2基因组与牛种、羊种野毒株基因组比较,筛选出S2疫苗特有基因trbJ作为检测靶标序列,设计LAMP特异性引物,在对该反应组分、反应条件进行单因素优化的同时,还对该引物特异性、灵敏度、重复性进行测试。结果显示,该方法在65℃等温条件下扩增,反应结束后加入SYBR Green I荧光染料,即可通过肉眼直接观察结果。该方法灵敏性、特异性、重复性良好,能准确鉴别出S2疫苗,与对照菌株无交叉反应。与普通PCR技术相比,LAMP检测灵敏度高100倍,最低可检测出6.89 pg/μL的DNA模板,并且不同批次试剂间的重复性良好。本试验成功建立了一种快速、准确、可视化地检测S2疫苗的LAMP方法。
