目的探讨脉管癌栓中间质细胞/癌细胞比值、巨噬细胞/癌细胞比值与pT 1~4 N 1~3 M 0期乳腺癌患者临床病理特征及预后的关系。方法采用回顾性研究方法,选取2013年1月至2015年12月在中国医科大学附属第一医院及大连医科大学附属第一、二医...目的探讨脉管癌栓中间质细胞/癌细胞比值、巨噬细胞/癌细胞比值与pT 1~4 N 1~3 M 0期乳腺癌患者临床病理特征及预后的关系。方法采用回顾性研究方法,选取2013年1月至2015年12月在中国医科大学附属第一医院及大连医科大学附属第一、二医院经系统治疗并有完整随访资料的167例pT 1~4 N 1~3 M 0期腋窝淋巴结阳性乳腺癌患者作为研究对象,中位随访55.2个月(16.8~69.6个月)。采用免疫组织化学染色方法分别检测脉管癌栓中间质细胞和巨噬细胞的数目,计算间质细胞/癌细胞比值及巨噬细胞/癌细胞比值,采用Ward法分别对间质细胞/癌细胞比值和巨噬细胞/癌细胞比值进行聚类,得到低比值和高比值两类。分别采用Kruskal-Wallis H检验、χ^2检验分析该比值与患者临床病理特征的关系,并用log-rank检验分析该比值与患者预后的关系。结果167例患者间质细胞/癌细胞、巨噬细胞/癌细胞比值的M(P 25~P 75)分别为0.025(0.015~0.063)和0.023(0.015~0.036)。间质细胞/癌细胞比值与患者的组织学分级、肿瘤大小、腋窝淋巴结转移状态、临床分期及脉管浸润有关(χ^2=10.210、6.210、64.315、56.901、19.771,P=0.001、0.045及P均<0.001),但与ER、PR、HER-2、Ki67表达及神经侵犯无关(χ^2=3.148、2.260、3.187、0.145、1.384,P=0.076、0.133、0.074、0.704、0.240);巨噬细胞/癌细胞比值与患者的肿瘤大小、ER表达、神经侵犯、组织学分级、腋窝淋巴结转移状态、临床分期、脉管浸润和HER-2表达有关(χ^2=7.487、6.680、8.439、13.002、25.856、31.394、19.771、27.921,P=0.024、0.010、0.004及P均<0.001),但与PR、Ki67表达无关(χ^2=3.616、1.183,P=0.057、0.277)。聚类后间质细胞/癌细胞比值与腋窝淋巴结转移状态及临床分期均有关系(χ^2=47.300,P<0.001;χ^2=14.260,P=0.001),而巨噬细胞/癌细胞比值仅与ER表达有关(χ^2=5.136,P=0.023)。间质细胞/癌细胞、巨噬细胞/癌细胞比值均与乳腺癌患者DFS率相关,比值越高者DFS率越低(预后越差)(χ^2=24.124、18.746,P均<0.001)。结论脉管癌栓中间质细胞/癌细胞比值和巨噬细胞/癌细胞比值高的患者预后不良,此比值可作为评价pT 1-4 N 1-3 M 0期乳腺癌患者复发风险的参考指标。展开更多
Superabsorbent polymers(SAPs) as soil moisture conditioners have been increasingly used in agriculture, but conflicting results were reported regarding the effects of SAPs on crop growth. In this study, both laborator...Superabsorbent polymers(SAPs) as soil moisture conditioners have been increasingly used in agriculture, but conflicting results were reported regarding the effects of SAPs on crop growth. In this study, both laboratory cultivation and analysis were conducted to investigate the effects of different SAPs on the growth and physiology of crops under water-saving agricultural practices. Maize(Zea mays L.) seedlings were cultivated using distilled water or three different SAP hydrogels, sodium polyacrylate(SP), potassium polyacrylate(PP), and sodium polyacrylate embedded with phosphate rock powder(SPP), as growth media. Growth characteristics of the model plant and damage were assessed using scanning electron microscopy(SEM) and transmission electron microscopy(TEM). The results showed that both the SP and PP treatments had pronounced negative effect on the hydrogels of growth of maize seedlings. The SPP treatment appeared to facilitate the stem-leaf growth and had no obvious adverse effect on root growth. All the three hydrogel treatments caused varying degrees of damage to the organizational structure and cellular morphology of the roots, with the SP and PP treatments causing the most severe damage; the membrane system of root cells was damaged by both SP and PP treatments. An excessive accumulation of sodium and reduction of calcium occurred in the roots may be responsible for the observed damage to the cell membrane system, which, in turn, may have promoted the wilting of the cells.展开更多
文摘目的探讨脉管癌栓中间质细胞/癌细胞比值、巨噬细胞/癌细胞比值与pT 1~4 N 1~3 M 0期乳腺癌患者临床病理特征及预后的关系。方法采用回顾性研究方法,选取2013年1月至2015年12月在中国医科大学附属第一医院及大连医科大学附属第一、二医院经系统治疗并有完整随访资料的167例pT 1~4 N 1~3 M 0期腋窝淋巴结阳性乳腺癌患者作为研究对象,中位随访55.2个月(16.8~69.6个月)。采用免疫组织化学染色方法分别检测脉管癌栓中间质细胞和巨噬细胞的数目,计算间质细胞/癌细胞比值及巨噬细胞/癌细胞比值,采用Ward法分别对间质细胞/癌细胞比值和巨噬细胞/癌细胞比值进行聚类,得到低比值和高比值两类。分别采用Kruskal-Wallis H检验、χ^2检验分析该比值与患者临床病理特征的关系,并用log-rank检验分析该比值与患者预后的关系。结果167例患者间质细胞/癌细胞、巨噬细胞/癌细胞比值的M(P 25~P 75)分别为0.025(0.015~0.063)和0.023(0.015~0.036)。间质细胞/癌细胞比值与患者的组织学分级、肿瘤大小、腋窝淋巴结转移状态、临床分期及脉管浸润有关(χ^2=10.210、6.210、64.315、56.901、19.771,P=0.001、0.045及P均<0.001),但与ER、PR、HER-2、Ki67表达及神经侵犯无关(χ^2=3.148、2.260、3.187、0.145、1.384,P=0.076、0.133、0.074、0.704、0.240);巨噬细胞/癌细胞比值与患者的肿瘤大小、ER表达、神经侵犯、组织学分级、腋窝淋巴结转移状态、临床分期、脉管浸润和HER-2表达有关(χ^2=7.487、6.680、8.439、13.002、25.856、31.394、19.771、27.921,P=0.024、0.010、0.004及P均<0.001),但与PR、Ki67表达无关(χ^2=3.616、1.183,P=0.057、0.277)。聚类后间质细胞/癌细胞比值与腋窝淋巴结转移状态及临床分期均有关系(χ^2=47.300,P<0.001;χ^2=14.260,P=0.001),而巨噬细胞/癌细胞比值仅与ER表达有关(χ^2=5.136,P=0.023)。间质细胞/癌细胞、巨噬细胞/癌细胞比值均与乳腺癌患者DFS率相关,比值越高者DFS率越低(预后越差)(χ^2=24.124、18.746,P均<0.001)。结论脉管癌栓中间质细胞/癌细胞比值和巨噬细胞/癌细胞比值高的患者预后不良,此比值可作为评价pT 1-4 N 1-3 M 0期乳腺癌患者复发风险的参考指标。
基金supported by the National Natural Science Foundation of China (Nos. 30600347 and 41071162)the Science and Technology Program of Guangzhou City, China (Nos. 201508030039 and 201604020074)
文摘Superabsorbent polymers(SAPs) as soil moisture conditioners have been increasingly used in agriculture, but conflicting results were reported regarding the effects of SAPs on crop growth. In this study, both laboratory cultivation and analysis were conducted to investigate the effects of different SAPs on the growth and physiology of crops under water-saving agricultural practices. Maize(Zea mays L.) seedlings were cultivated using distilled water or three different SAP hydrogels, sodium polyacrylate(SP), potassium polyacrylate(PP), and sodium polyacrylate embedded with phosphate rock powder(SPP), as growth media. Growth characteristics of the model plant and damage were assessed using scanning electron microscopy(SEM) and transmission electron microscopy(TEM). The results showed that both the SP and PP treatments had pronounced negative effect on the hydrogels of growth of maize seedlings. The SPP treatment appeared to facilitate the stem-leaf growth and had no obvious adverse effect on root growth. All the three hydrogel treatments caused varying degrees of damage to the organizational structure and cellular morphology of the roots, with the SP and PP treatments causing the most severe damage; the membrane system of root cells was damaged by both SP and PP treatments. An excessive accumulation of sodium and reduction of calcium occurred in the roots may be responsible for the observed damage to the cell membrane system, which, in turn, may have promoted the wilting of the cells.
