Objective To contrast and analyze the difference of metabolites of intestinal flora between patients with type 2diabetes and normal blood glucose human in Xinjiang Uygur and Kazak.Methods Uygur fecal samples and Kazak...Objective To contrast and analyze the difference of metabolites of intestinal flora between patients with type 2diabetes and normal blood glucose human in Xinjiang Uygur and Kazak.Methods Uygur fecal samples and Kazak fecal samples were collected,and short-chain fatty acids(SCFA)were extracted by water from feces,then the content of SCFA in fecal samples was analyzed by gas-chromatography mass-spectrometry(GC-MS).Results Both Uygurs and Kazaks,in their feces,the content of SCFA in the feces showed significantly decreased in patients with type 2diabetes,and a significant negative linear correlation was shown between the fasting plasma glucose and the concentration of SCFA in the fecal samples.But,there was no statistical differences between uygurs and kazaks,in not only normal blood glucose group,but also type 2 diabetes group.Conclusion The feces of patients with type 2diabetes has an decreased concentration of SCFA.Between Uygurs and Kazaks,the metabolic changes of intestinal flora was similar in patients with type 2diabetes.展开更多
Objective To determine the effects of genetic variation in the organic cation transporter 1(OCT1)on the short-term responses of the antidiabetic drug,metformin.Method A total of 22 patients recruited with type 2 diabe...Objective To determine the effects of genetic variation in the organic cation transporter 1(OCT1)on the short-term responses of the antidiabetic drug,metformin.Method A total of 22 patients recruited with type 2 diabetes or IFG were treated with metformin(2 000 mg/day)for 1 week.The patients were screened from Second Jikun hospital and Kashidonglu community medicine service,Urumqi,China and their surrounding districts.To examine the effects of metformin on plasma glucose,total cholesterol,low-density lipoprotein-cholesterol,high-density lipoprotein-cholesterol and triglyceride in relation with R61C,G465R and 420 del variants of OCT1(gene encoding organic cation transporter 1,mainly locating in liver,which is metformin's major target)in subjects.In all,R61C,G465R and 420del of OCT1 gene were examined using DNA extracted from whole blood and PCR-RFLP.Data concerning with gene and metformin treatment were handled by t-test.Result After metformin treatment,there were increases both in FPG and LDL(P=0.011and P=0.013 respectively).To divide all participants into mutant and wild groups,according to the polymorphisms of R61C,G465R and 420 del respectively,as well as carriers with one of the mutant genotypes at least and carriers with none of the mutant sites.Analysis was made to compared FPG,Chol,TG,and LDL and HDL between carriers of wild genotypes and carriers of other genotypes showed no statistic significance both before the metformin treatment and after the treatment.The same is the case with changes of FPG,Chol,TG,and LDL and HDL of wild genotype carriers and variant genotype carriers,except of LDL changes(P=0.05)in patients grouped by G465R polymorphisms and TG changes(P=0.03)in subjects differed by 420del genotypes.Conclusion In this study,it is suggested that OCT1 gene polymorphisms have little contribution to the clinical efficacy of blood glucose control by metformin among Uygur people with type 2 diabetes or IFG,but it may have possible relationship with the clinical efficacy on fat metabolism by metformin.展开更多
Background:To investigate the effect of body mass index(BMI)on the outcomes of in vitro fertilization–embryo transfer(IVF-ET)treatment,and to provide evidence for future clinical studies of infertile women with diffe...Background:To investigate the effect of body mass index(BMI)on the outcomes of in vitro fertilization–embryo transfer(IVF-ET)treatment,and to provide evidence for future clinical studies of infertile women with different body mass indices.Methods:The relationship between pregnancy outcome and BMI in 717 infertile women who underwent IVF-ET was analyzed.Results:A total of 531 out of 717 patients were classified as having a standard body weight,and 20 patients were classified as obese.We found a significant difference in the basal follicle-stimulating hormone(FSH)level among the groups;the basal FSH level was lower in the obese group(6.80±2.20 IU/L)than in the standard weight group(7.56±2.25 IU/L).However,there were no significant differences in the age of females,the age of males,basal estradiol(E2)and luteinizing hormone(LH)levels,length of infertility,and history of dysmenorrhea.Although the pregnancy rate was highest(54.6%)in the standard weight group and lowest(45.0%)in the obese group,there was no significant difference among these groups by Chi-square test analysis.Furthermore,there were no significant differences in gonadotropin-releasing hormone agonist days,number of 2PN,and the LH level on the day of human chorionic gonadotropin treatment among the groups.Conclusions:Obese women can improve a successful pregnancy rate after undergoing IVF–ET treatment by controlling weight.展开更多
基金National natural science foundation project(NO.30760288)
文摘Objective To contrast and analyze the difference of metabolites of intestinal flora between patients with type 2diabetes and normal blood glucose human in Xinjiang Uygur and Kazak.Methods Uygur fecal samples and Kazak fecal samples were collected,and short-chain fatty acids(SCFA)were extracted by water from feces,then the content of SCFA in fecal samples was analyzed by gas-chromatography mass-spectrometry(GC-MS).Results Both Uygurs and Kazaks,in their feces,the content of SCFA in the feces showed significantly decreased in patients with type 2diabetes,and a significant negative linear correlation was shown between the fasting plasma glucose and the concentration of SCFA in the fecal samples.But,there was no statistical differences between uygurs and kazaks,in not only normal blood glucose group,but also type 2 diabetes group.Conclusion The feces of patients with type 2diabetes has an decreased concentration of SCFA.Between Uygurs and Kazaks,the metabolic changes of intestinal flora was similar in patients with type 2diabetes.
基金The National Natural Science Foundation of China(30760288)Xinjiang Uygur Autonomic Tackle Key Problems Plans(200633129)Science and Technology Program of Urumqi(G07231001)
文摘Objective To determine the effects of genetic variation in the organic cation transporter 1(OCT1)on the short-term responses of the antidiabetic drug,metformin.Method A total of 22 patients recruited with type 2 diabetes or IFG were treated with metformin(2 000 mg/day)for 1 week.The patients were screened from Second Jikun hospital and Kashidonglu community medicine service,Urumqi,China and their surrounding districts.To examine the effects of metformin on plasma glucose,total cholesterol,low-density lipoprotein-cholesterol,high-density lipoprotein-cholesterol and triglyceride in relation with R61C,G465R and 420 del variants of OCT1(gene encoding organic cation transporter 1,mainly locating in liver,which is metformin's major target)in subjects.In all,R61C,G465R and 420del of OCT1 gene were examined using DNA extracted from whole blood and PCR-RFLP.Data concerning with gene and metformin treatment were handled by t-test.Result After metformin treatment,there were increases both in FPG and LDL(P=0.011and P=0.013 respectively).To divide all participants into mutant and wild groups,according to the polymorphisms of R61C,G465R and 420 del respectively,as well as carriers with one of the mutant genotypes at least and carriers with none of the mutant sites.Analysis was made to compared FPG,Chol,TG,and LDL and HDL between carriers of wild genotypes and carriers of other genotypes showed no statistic significance both before the metformin treatment and after the treatment.The same is the case with changes of FPG,Chol,TG,and LDL and HDL of wild genotype carriers and variant genotype carriers,except of LDL changes(P=0.05)in patients grouped by G465R polymorphisms and TG changes(P=0.03)in subjects differed by 420del genotypes.Conclusion In this study,it is suggested that OCT1 gene polymorphisms have little contribution to the clinical efficacy of blood glucose control by metformin among Uygur people with type 2 diabetes or IFG,but it may have possible relationship with the clinical efficacy on fat metabolism by metformin.
基金The Natural Science Foundation of Xinjiang Uyghur Autonomous Region(No.2013211A087).
文摘Background:To investigate the effect of body mass index(BMI)on the outcomes of in vitro fertilization–embryo transfer(IVF-ET)treatment,and to provide evidence for future clinical studies of infertile women with different body mass indices.Methods:The relationship between pregnancy outcome and BMI in 717 infertile women who underwent IVF-ET was analyzed.Results:A total of 531 out of 717 patients were classified as having a standard body weight,and 20 patients were classified as obese.We found a significant difference in the basal follicle-stimulating hormone(FSH)level among the groups;the basal FSH level was lower in the obese group(6.80±2.20 IU/L)than in the standard weight group(7.56±2.25 IU/L).However,there were no significant differences in the age of females,the age of males,basal estradiol(E2)and luteinizing hormone(LH)levels,length of infertility,and history of dysmenorrhea.Although the pregnancy rate was highest(54.6%)in the standard weight group and lowest(45.0%)in the obese group,there was no significant difference among these groups by Chi-square test analysis.Furthermore,there were no significant differences in gonadotropin-releasing hormone agonist days,number of 2PN,and the LH level on the day of human chorionic gonadotropin treatment among the groups.Conclusions:Obese women can improve a successful pregnancy rate after undergoing IVF–ET treatment by controlling weight.
