背景:由于金银双金属纳米粒子较强的催化活性及选择性催化氧化的特性,在生物医学、药物传递、电化学分析等方面存在潜在的广泛应用。目的:以动物细胞中线粒体为模板,制备金银合金纳米粒子。方法:从鱼肝中分离线粒体;在1 m L偏碱性HAu Cl...背景:由于金银双金属纳米粒子较强的催化活性及选择性催化氧化的特性,在生物医学、药物传递、电化学分析等方面存在潜在的广泛应用。目的:以动物细胞中线粒体为模板,制备金银合金纳米粒子。方法:从鱼肝中分离线粒体;在1 m L偏碱性HAu Cl4溶液(10 mmol/L)中加入1 m L线粒体母液,摇匀,加入1 m L Ag NO3溶液(10 mmol/L),室温条件下在电磁搅拌器上反应20-30 h,观察反应液颜色从无色逐渐变为紫色,即得金银合金纳米粒子,对其进行表征。(1)细胞毒性实验:将不同质量浓度(0,25,50,75,100,125,150 mg/L)的金银合金纳米粒子加入到胃癌细胞中,培养48 h后,MTT法检测细胞增殖A值;(2)安全稳定性实验:将1 m L不同质量浓度(0,25,50,75,100,125,150 mg/L)的金银合金纳米粒子溶液置于玻璃反应瓶中,将0.2 m L超纯水分次加入到纳米粒子溶液中,左右震荡摇匀;当加入超纯水后,使用紫外-可见光谱对溶液进行表征。结果与结论:(1)金银合金纳米粒子为合金结构,平均粒径20-30 nm,基本为球形分布,粒度较均一,结晶度较好,结构单一,表面存在羟基、羰基等活性基团;(2)MTT实验表明在150 mg/L质量浓度范围内,金银纳米粒子无明显的细胞毒性;(3)随着金银合金纳米粒子质量浓度的变化,强度最大的特征吸收峰与金银合金纳米颗粒的含量基本呈正相关;(4)结果表明,金银合金纳米粒子具有良好的细胞相容性及体外稳定性。展开更多
In present paper, the contour deletion method is developed both to blend surfaces and to fill N-sided holes, which is used for subdividing the NURBS surface. First, according to the non-uniform Catmull-Clark subdivisi...In present paper, the contour deletion method is developed both to blend surfaces and to fill N-sided holes, which is used for subdividing the NURBS surface. First, according to the non-uniform Catmull-Clark subdivision principle, surfaces are blended. The non-uniform Catmull-Clark subdivision method is constructed, which build the surface through interpolating comer vertices and boundary curves. Then the contour deletion method is adapted to remove the controlling mesh boundary contour in the process of segmentation iteration. Last, N sided-hole is filled to generate a integral smooth continuous surface. This method not only guarantee that the blending surface and base surface patches have C2 continuity at the boundary, but also greatly improve the smoothness of the N-side hole filling surface. The results show that, this method simplifies the specific computer-implemented process, broads the scope of application of subdivision surfaces, and solves the incompatible problem between the subdivision surface and classical spline. The resulting surface has both advantages of the subdivision surface and classical spline, and also has better filling effect.展开更多
文摘背景:由于金银双金属纳米粒子较强的催化活性及选择性催化氧化的特性,在生物医学、药物传递、电化学分析等方面存在潜在的广泛应用。目的:以动物细胞中线粒体为模板,制备金银合金纳米粒子。方法:从鱼肝中分离线粒体;在1 m L偏碱性HAu Cl4溶液(10 mmol/L)中加入1 m L线粒体母液,摇匀,加入1 m L Ag NO3溶液(10 mmol/L),室温条件下在电磁搅拌器上反应20-30 h,观察反应液颜色从无色逐渐变为紫色,即得金银合金纳米粒子,对其进行表征。(1)细胞毒性实验:将不同质量浓度(0,25,50,75,100,125,150 mg/L)的金银合金纳米粒子加入到胃癌细胞中,培养48 h后,MTT法检测细胞增殖A值;(2)安全稳定性实验:将1 m L不同质量浓度(0,25,50,75,100,125,150 mg/L)的金银合金纳米粒子溶液置于玻璃反应瓶中,将0.2 m L超纯水分次加入到纳米粒子溶液中,左右震荡摇匀;当加入超纯水后,使用紫外-可见光谱对溶液进行表征。结果与结论:(1)金银合金纳米粒子为合金结构,平均粒径20-30 nm,基本为球形分布,粒度较均一,结晶度较好,结构单一,表面存在羟基、羰基等活性基团;(2)MTT实验表明在150 mg/L质量浓度范围内,金银纳米粒子无明显的细胞毒性;(3)随着金银合金纳米粒子质量浓度的变化,强度最大的特征吸收峰与金银合金纳米颗粒的含量基本呈正相关;(4)结果表明,金银合金纳米粒子具有良好的细胞相容性及体外稳定性。
基金Supported by NUAA Fundamental Research Funds(NZ2013201)
文摘In present paper, the contour deletion method is developed both to blend surfaces and to fill N-sided holes, which is used for subdividing the NURBS surface. First, according to the non-uniform Catmull-Clark subdivision principle, surfaces are blended. The non-uniform Catmull-Clark subdivision method is constructed, which build the surface through interpolating comer vertices and boundary curves. Then the contour deletion method is adapted to remove the controlling mesh boundary contour in the process of segmentation iteration. Last, N sided-hole is filled to generate a integral smooth continuous surface. This method not only guarantee that the blending surface and base surface patches have C2 continuity at the boundary, but also greatly improve the smoothness of the N-side hole filling surface. The results show that, this method simplifies the specific computer-implemented process, broads the scope of application of subdivision surfaces, and solves the incompatible problem between the subdivision surface and classical spline. The resulting surface has both advantages of the subdivision surface and classical spline, and also has better filling effect.
