Bacillus subtilis B47 is an endophytic bacterium of tomato, and produces substance that strongly inhibits the growth of Bipolaris maydis, the pathogen of southern corn leaf blight （SCLB）, as well as several other ph...Bacillus subtilis B47 is an endophytic bacterium of tomato, and produces substance that strongly inhibits the growth of Bipolaris maydis, the pathogen of southern corn leaf blight （SCLB）, as well as several other phytopathogenic fungi. The antifungal substance was purified from the broth culture of the bacterium using acid precipitation, methanol extraction, and three-step chromatography. Based on FT-IR spectrometry, amino acid composition, and MALDI-TOF-MS/MS CID analyses, the antifungal substance was identified as iturin A2, a cyclic lipopeptide antibiotic. To evaluate the efficacy of iturin A2 for control of SCLB, partially purified iturin A2 （75%, w/v） was applied under different conditions. At the concentration of 300 mg kg-1, iturin A2 showed efficacy ranging from 100 to 53.1% under in vitro, in plot and in field conditions. This efficacy was higher than or similar to that of the fungicide chlorothalonil. When the concentration of iturin A2 was increased to 500 mg kg-1, the control efficacy was enhanced to 64.2% in field, which was significantly higher than that of chlorothalonil. These results indicate that iturin A2 has potential for SCLB control and could be a substitute to synthetic fungicides. To our knowledge, this is the first report on using partially purified iturin A for control of SCLB under field conditions.展开更多
目的探索改良骨牵引器闭合复位在老年转子间骨折股骨近端防旋髓内钉(proximal femoral nail antirotation,PFNA)内固定术中的应用效果。方法将郑州大学附属郑州中心医院2019年1月—2020年6月期间收治的外伤性股骨转子间骨折患者81例作...目的探索改良骨牵引器闭合复位在老年转子间骨折股骨近端防旋髓内钉(proximal femoral nail antirotation,PFNA)内固定术中的应用效果。方法将郑州大学附属郑州中心医院2019年1月—2020年6月期间收治的外伤性股骨转子间骨折患者81例作为研究对象,根据复位方法将患者分为观察组(骨牵引器辅助复位,41例)和对照组(牵引床辅助复位,40例),两组均行持续硬膜外麻醉联合蛛网膜下腔阻滞麻醉下PFNA内固定术治疗,观察组行骨牵引器辅助复位,术后随访一年,对照组行牵引床辅助复位,比较两组手术相关指标,术后肢体功能及疼痛、康复状况、并发症发生率。结果和对照组比较,观察组术中体位摆放时间、透视次数、手术时间、术中出血量均得到明显改善,两组比较差异均有统计学意义(P均<0.05),而球形顶锥或克氏针使用率两组比较则无明显差异(P>0.05)。组间比较发现两组术后1周和术后12个月Harris评分及VAS评分均无明显差异(均P>0.05),组内比较发现,两组术后12个月Harris评分均较术后7d增加,VAS评分均较术后1周降低(P均<0.05)。两组患者住院时间,开始下床活动时间,去拐行走时间,骨折愈合时间及并发症发生率比较差异均无统计学意义(均P>0.05)。结论老年转子间骨折患者在采用PFNA治疗时应用牵引器辅助复位较牵引床复位操作简单,术中体位摆放时间、透视次数、手术时间、术中出血量更少,不会损伤会阴,值得推广应用。展开更多
基金financially supported by the grants from the Department of Science and Technology,Guangxi Zhuang Autonomous Region,China (0991056)
文摘Bacillus subtilis B47 is an endophytic bacterium of tomato, and produces substance that strongly inhibits the growth of Bipolaris maydis, the pathogen of southern corn leaf blight （SCLB）, as well as several other phytopathogenic fungi. The antifungal substance was purified from the broth culture of the bacterium using acid precipitation, methanol extraction, and three-step chromatography. Based on FT-IR spectrometry, amino acid composition, and MALDI-TOF-MS/MS CID analyses, the antifungal substance was identified as iturin A2, a cyclic lipopeptide antibiotic. To evaluate the efficacy of iturin A2 for control of SCLB, partially purified iturin A2 （75%, w/v） was applied under different conditions. At the concentration of 300 mg kg-1, iturin A2 showed efficacy ranging from 100 to 53.1% under in vitro, in plot and in field conditions. This efficacy was higher than or similar to that of the fungicide chlorothalonil. When the concentration of iturin A2 was increased to 500 mg kg-1, the control efficacy was enhanced to 64.2% in field, which was significantly higher than that of chlorothalonil. These results indicate that iturin A2 has potential for SCLB control and could be a substitute to synthetic fungicides. To our knowledge, this is the first report on using partially purified iturin A for control of SCLB under field conditions.
文摘目的探索改良骨牵引器闭合复位在老年转子间骨折股骨近端防旋髓内钉(proximal femoral nail antirotation,PFNA)内固定术中的应用效果。方法将郑州大学附属郑州中心医院2019年1月—2020年6月期间收治的外伤性股骨转子间骨折患者81例作为研究对象,根据复位方法将患者分为观察组(骨牵引器辅助复位,41例)和对照组(牵引床辅助复位,40例),两组均行持续硬膜外麻醉联合蛛网膜下腔阻滞麻醉下PFNA内固定术治疗,观察组行骨牵引器辅助复位,术后随访一年,对照组行牵引床辅助复位,比较两组手术相关指标,术后肢体功能及疼痛、康复状况、并发症发生率。结果和对照组比较,观察组术中体位摆放时间、透视次数、手术时间、术中出血量均得到明显改善,两组比较差异均有统计学意义(P均<0.05),而球形顶锥或克氏针使用率两组比较则无明显差异(P>0.05)。组间比较发现两组术后1周和术后12个月Harris评分及VAS评分均无明显差异(均P>0.05),组内比较发现,两组术后12个月Harris评分均较术后7d增加,VAS评分均较术后1周降低(P均<0.05)。两组患者住院时间,开始下床活动时间,去拐行走时间,骨折愈合时间及并发症发生率比较差异均无统计学意义(均P>0.05)。结论老年转子间骨折患者在采用PFNA治疗时应用牵引器辅助复位较牵引床复位操作简单,术中体位摆放时间、透视次数、手术时间、术中出血量更少,不会损伤会阴,值得推广应用。
