Investigation on the differences of four flavonoids with similar structure binding to human serum albumin

Flavonoids are structurally diverse and the most ubiquitous groups of polyphenols distributed in the various plants,which possess intensive biological activities.In this study,the interaction mechanisms between four flavonoids containing one glucose unit with similar molecular weight isolated from the Tibetan medicinal herb Pyrethrum tatsienense,namely.apigenin-7-O-β-D-glucoside（1）,luteolin-7-O-β-D-glucoside（2）.quercetin-7-O-β-D-glucoside（3）.quercetin-3-O-β-D-glycoside（4）.and human serum albumin（HSA）,were investigated by fluorescence.UV-vis absorbance,circular dichroism,and molecular modeling.The effects of biological metal ions Mg2＋,Zn2＋,and Cu2＋ on the binding affinity between flavonoids and HSA were further examined.Structure-activity relationships of four flavonoids binding to HSA were discussed in depth and some meaningful conclusions have been drawn by the experiment data and theoretical simulation.In addition,an interesting phenomenon was observed that the microenvironment of the binding site I in HSA has hardly changed in the presence of 4 differentiating from the other three flavonoids on the basis of conformation investigations.

Rules Mining-Based Gene Expression Programming for the Multi-Skill Resource Constrained Project Scheduling Problem

Themulti-skill resource-constrained project scheduling problem(MS-RCPSP)is a significantmanagement science problem that extends from the resource-constrained project scheduling problem(RCPSP)and is integrated with a real project and production environment.To solve MS-RCPSP,it is an efficient method to use dispatching rules combined with a parallel scheduling mechanism to generate a scheduling scheme.This paper proposes an improved gene expression programming(IGEP)approach to explore newly dispatching rules that can broadly solve MS-RCPSP.A new backward traversal decoding mechanism,and several neighborhood operators are applied in IGEP.The backward traversal decoding mechanism dramatically reduces the space complexity in the decoding process,and improves the algorithm’s performance.Several neighborhood operators improve the exploration of the potential search space.The experiment takes the intelligent multi-objective project scheduling environment(iMOPSE)benchmark dataset as the training set and testing set of IGEP.Ten newly dispatching rules are discovered and extracted by IGEP,and eight out of ten are superior to other typical dispatching rules.

Association of daily sitting time and leisure-time physical activity with body fat among U.S.adults

Background:Prolonged sitting and reduced physical activity lead to low energy expenditures.However,little is known about the joint impact of daily sitting time and physical activity on body fat distribution.We investigated the independent and joint associations of daily sitting time and physical activity with body fat among adults.Methods:This was a cross-sectional analysis of U.S.nationally representative data from the National Health and Nutrition Examination Survey2011-2018 among adults aged 20 years or older.Daily sitting time and leisure-time physical activity(LTPA)were self-reported using the Global Physical Activity Questionnaire.Body fat(total and trunk fat percentage)was determined via dual X-ray absorptiometry.Results:Among 10,808 adults,about 54.6%spent 6 h/day or more sitting;more than one-half reported no LTPA(inactive)or less than 150 min/week LTPA(insufficiently active)with only 43.3%reported 150 min/week or more LTPA(active)in the past week.After fully adjusting for sociodemographic data,lifestyle behaviors,and chronic conditions,prolonged sitting time and low levels of LTPA were associated with higher total and trunk fat percentages in both sexes.When stratifying by LTPA,the association between daily sitting time and body fat appeared to be stronger in those who were inactive/insuufficiently active.In the joint analyses,inactive/insuufficiently active adults who reported sitting more than 8 h/day had the highest total(female:3.99%(95%confidence interval(95%CI):3.09%-4.88%);male:3.79%(95%CI:2.75%-4.82%))and trunk body fat percentages(female:4.21%(95%CI:3.09%-5.32%);male:4.07%(95%CI:2.95%-5.19%))when compared with those who were active and sitting less than 4 h/day.Conclusion:Prolonged daily sitting time was associated with increased body fat among U.S.adults.The higher body fat associated with 6 h/day sitting may not be offset by achieving recommended levels of physical activity.

Expert consensus on pediatric orthodontic therapies of malocclusions in children

Malocclusion,identified by the World Health Organization(WHO)as one of three major oral diseases,profoundly impacts the dental-maxillofacial functions,facial esthetics,and long-term development of~260 million children in China.Beyond its physical manifestations,malocclusion also significantly influences the psycho-social well-being of these children.Timely intervention in malocclusion can foster an environment conducive to dental-maxillofacial development and substantially decrease the incidence of malocclusion or reduce the severity and complexity of malocclusion in the permanent dentition,by mitigating the negative impact of abnormal environmental influences on the growth.Early orthodontic treatment encompasses accurate identification and treatment of dental and maxillofacial morphological and functional abnormalities during various stages of dental-maxillofacial development,ranging from fetal stages to the early permanent dentition phase.From an economic and societal standpoint,the urgency for effective early orthodontic treatments for malocclusions in childhood cannot be overstated,underlining its profound practical and social importance.This consensus paper discusses the characteristics and the detrimental effects of malocclusion in children,emphasizing critical need for early treatment.It elaborates on corresponding core principles and fundamental approaches in early orthodontics,proposing comprehensive guidance for preventive and interceptive orthodontic treatment,serving as a reference for clinicians engaged in early orthodontic treatment.

A 48-bp deletion upstream of LIGULELESS 1 alters rice panicle architecture

Panicle architecture is an agronomic determinant of crop yield and a target for cereal crop improvement.To investigate its molecular mechanisms in rice,we performed map-based cloning and characterization of OPEN PANICLE 1(OP1),a gain-of-function allele of LIGULELESS 1(LG1),controlling the spread-panicle phenotype.This allele results from a 48-bp deletion in the LG1 upstream region and promotes pulvinus development at the base of the primary branch.Increased OP1 expression and altered panicle phenotype in chimeric transgenic plants and upstream-region knockout mutants indicated that the deletion regulates spread-panicle architecture in the mutant spread panicle 1(sp1).Knocking out BRASSINOSTEROID UPREGULATED1(BU1)gene in the background of OP1 complementary plants resulted in compact panicles,suggesting OP1 may regulate inflorescence architecture via the brassinosteroid signaling pathway.We regard that manipulating the upstream regulatory region of OP1 or genes involved in BR signal pathway could be an efficient way to improve rice inflorescence architecture.

Fine-Grained Ship Recognition Based on Visible and Near-Infrared Multimodal Remote Sensing Images: Dataset,Methodology and Evaluation

Fine-grained recognition of ships based on remote sensing images is crucial to safeguarding maritime rights and interests and maintaining national security.Currently,with the emergence of massive high-resolution multi-modality images,the use of multi-modality images for fine-grained recognition has become a promising technology.Fine-grained recognition of multi-modality images imposes higher requirements on the dataset samples.The key to the problem is how to extract and fuse the complementary features of multi-modality images to obtain more discriminative fusion features.The attention mechanism helps the model to pinpoint the key information in the image,resulting in a significant improvement in the model’s performance.In this paper,a dataset for fine-grained recognition of ships based on visible and near-infrared multi-modality remote sensing images has been proposed first,named Dataset for Multimodal Fine-grained Recognition of Ships(DMFGRS).It includes 1,635 pairs of visible and near-infrared remote sensing images divided into 20 categories,collated from digital orthophotos model provided by commercial remote sensing satellites.DMFGRS provides two types of annotation format files,as well as segmentation mask images corresponding to the ship targets.Then,a Multimodal Information Cross-Enhancement Network(MICE-Net)fusing features of visible and near-infrared remote sensing images,has been proposed.In the network,a dual-branch feature extraction and fusion module has been designed to obtain more expressive features.The Feature Cross Enhancement Module(FCEM)achieves the fusion enhancement of the two modal features by making the channel attention and spatial attention work cross-functionally on the feature map.A benchmark is established by evaluating state-of-the-art object recognition algorithms on DMFGRS.MICE-Net conducted experiments on DMFGRS,and the precision,recall,mAP0.5 and mAP0.5:0.95 reached 87%,77.1%,83.8%and 63.9%,respectively.Extensive experiments demonstrate that the proposed MICE-Net has more excellent performance on DMFGRS.Built on lightweight network YOLO,the model has excellent generalizability,and thus has good potential for application in real-life scenarios.

Retardation of myopia by atropine regimes

Myopia is a huge health problem due to its high frequency,vision losses and public health cost.According to the World Health Organization,at least 2.2 billion people have vision impairment.Although myopia can be controlled at its early and middle stages,unfortunately,no cure can be achieved so far.Among the methods to control myopia,atropine,a muscarinic receptor antagonist,is the oldest but still the most effective for retardation of myopia progression.Despite such a fact,standard protocols have not been established for clinicians to use atropine for treatment of myopia.In this article,a concise and up to date summary of myopia epidemiology and pathogenesis and summarized therapeutic effects and side effects,possible mechanisms and application methods of atropine were provided in hope for clinical doctors to effectively control this problematic disease.At present,the protocol is recommend:use higher dose(1%)of atropine intermittently to effectively slowdown myopia progression in schoolchildren for 2y,and to significantly reduce side effects of atropine by decrease of atropine frequency for 1y and inhibit myopic rebound by withdrawal of topical atropine gradually for 1y.Application of a lower dose(0.05%)atropine regime should also be considered due to its effectiveness and application at regular basis.

急性髓细胞白血病患者CD96和CD123的表达及对预后的意义

目的检测CD96、CD123在急性髓细胞白血病(AML)患者中的表达,探讨其与AML疗效和预后的关系。方法选取156例AML患者为观察对象,选取同期该院健康体检者150例作为对照组。采用流式细胞术检测CD96、CD123表达情况,并分析CD96、CD123表达与化疗疗效的关系。结果 AML患者CD96、CD123的阳性表达率与对照组比较,差异有统计学意义(P <0.05);AML患者中CD96、CD123阳性表达的缓解率均低于阴性表达者(P <0.05);AML患者中CD96、CD123阳性表达的生存率均低于阴性表达者(P <0.05);AML患者中CD96、CD123阳性表达的化疗后感染、化疗后复发发生率均高于阴性表达者(P <0.05);AML患者中CD96、CD123阳性表达的中位生存期均低于阴性表达者(P<0.05)。结论 CD96、CD123在AML中均有不同程度的表达,其阳性表达可能与原发耐药或复发进展有关,可作为判断AML患者预后的相关指标。

交联改性对静电纺乌拉草提取液/聚乙烯醇纳米纤维膜性能的影响

为了优化用于医用敷料的乌拉草提取液/聚乙烯醇纳米纤维膜的耐水性,对制备好的纤维膜进行气相交联改性。选用戊二醛和浓盐酸作为交联剂和催化剂,在满足医用敷料性能的基础上提高膜的耐水性,进而提高抗菌性,并研究交联时间对膜的影响,确定最优交联时间。采用扫描电子显微镜、电子单纤强力仪及红外光谱仪对改性前后膜的微观形貌、力学性能及分子结构进行表征,并对膜的透气性和孔隙率、接触角和吸水性以及抗菌性进行测试。结果表明,经戊二醛交联改性后,纳米纤维膜的接触角从16°逐渐增大到78°,耐水性显著提高;其形貌、水蒸气透过率及孔隙率均能满足医用敷料的要求。随着交联时间的延长,其力学性能和吸水倍率先增强后减弱,在交联4 h时分别达到最大值(7.34±0.80)MPa和857%;其抗菌性在交联2 h时达到最优,此时大肠杆菌和金黄色葡萄球菌的抑菌圈直径分别为3.0 mm和1.1 mm。由以上结论得出,交联2 h和4 h的纳米纤维膜的综合性能最好。

Translational exercise biomedicine–where do we go?

As the concept of“exercise is medicine”has grown in recent years,there is an urgent need for translational research in thefield of exercise science that bridges the gaps between basic scientific discoveries(e.g.,detailing the physiological,biological and molecular responses to exercise)and its application to improving human health and targeting specific disease pathophysiology.Translational research is also needed to bridge the gap between clinical trials describing the health benefits of exercise and the underlying mechanisms to change and optimize clinical practice,health promotion,and exercise guidelines.Therefore,with the new peer-reviewed,open-access academic journal“Translational Exercise Biomedicine”,we aim to further personalize and advance exercise prescription and improve physically active lifestyles for prevention and therapy.

输尿管导管辅助软镜工作内鞘扩张输尿管在二期软镜碎石术中置鞘困难时的应用

目的探讨输尿管导管辅助软镜工作内鞘扩张输尿管在二期输尿管软镜碎石术中置鞘困难时的临床应用。方法对一期放置5F输尿管支架管、二期行输尿管软镜碎石术时置鞘困难的9例患者,采用5F输尿管导管辅助12F软镜工作内鞘扩张输尿管后置鞘并行输尿管软镜钬激光碎石术。结果经扩张后的9例患者,软镜工作鞘均置于满意位置并成功碎石,未出现严重并发症。术后随访6个月未发现输尿管狭窄。结论应用输尿管导管辅助软镜工作内鞘扩张输尿管对二期行输尿管软镜碎石术置鞘困难的患者有提高手术成功率、降低手术风险、减轻经济负担的作用,是二期行输尿管软镜碎石术出现置鞘困难时有效、便捷、安全的辅助手段。

间充质干细胞来源的胞外囊泡在急性肝衰竭治疗中的研究进展

急性肝衰竭(Acute liver failure,ALF)是指无肝脏基础疾病而在短时间内发生大量肝细胞坏死及严重肝功能损害,并引起多器官障碍的危重临床综合征。ALF的病死率较高,但临床上还没有特效的治疗方法。因此,开发新的治疗手段来有效缓解肝衰竭的进展是研究者普遍关注且亟需解决的关键问题。目前临床前研究的方法中,干细胞疗法被认为是治疗肝衰竭最有潜力的方法。但干细胞固有的临床风险阻滞了它的进一步应用,加上越来越多的证据表明,干细胞的生物学功能在很大程度上归功于其分泌的胞外囊泡(Extracellular vesicles,EVs)。EVs作为细胞自然产生的纳米级囊泡,已经成为ALF治疗中最具吸引力的纳米治疗手段之一。本文主要综述了EVs的形成、组分与分离,以及EVs在ALF治疗中的潜在机制。

不同经上皮角膜胶原交联方法治疗进展期圆锥角膜的早期疗效观察

目的:观察比较不同经上皮角膜胶原交联方法(transepithelial corneal collagen cross-linking,TE-CXL)治疗进展期圆锥角膜的早期疗效。方法:回顾性研究。将24例34眼进展期圆锥角膜分为三组,低渗CXL组10眼接受低渗胶原交联治疗,I-CXL5min组14眼接受离子导入5min胶原交联治疗,I-CXL10min组10眼接受离子导入10min胶原交联治疗。治疗前,治疗后1wk,1、3、6mo观察视力、Pentacam眼前节分析仪、角膜激光共焦显微镜、光学相关断层扫描结果变化。结果:术后6mo,I-CXL 10min组CDVA(矫正远视力,LogM AR)提高-0.21±0.23(t=2.735,P=0.026);最大角膜屈光力(Kmax)降低2.32±5.21D,但差异无统计学意义(t=1.40,P=0.193),低渗CXL组与I-CXL 5min组的UDVA、CDVA、Kmax稳定,差异均无统计学意义(P>0.05)。术后1wk时,分界线平均深度在各组分别为:低渗CXL组152.7±42.9μm,I-CXL 5min组213.6±42.3μm,I-CXL10min组237.0±46.4μm,组间比较,差异有统计学意义(F=7.111,P=0.006)。术后基质细胞的凋亡-活化-再生现象在I-CXL 10min组最明显。三组角膜最薄点厚度、角膜内皮细胞密度与术前比较,差异无统计学意义(P>0.05)。结论:三种经上皮CXL短期观察均能安全有效控制圆锥角膜病情的发展,其中离子导入10min胶原交联方法组织反应更显著。

荧光碳点的制备及其荧光特性的研究和应用

围绕具有优良荧光发光特性的无机纳米材料——碳点,设计一个本科化学实验。通过"自下而上"的方法分别制备蓝色、绿色荧光碳点溶液;考察荧光碳点溶液的荧光激发和发射光谱关系,相对法测定荧光量子产率;探究不同浓度和pH条件下的荧光光谱变化;并将荧光碳点应用到防伪技术领域,加深学生对荧光材料的相关荧光概念和表征手段如荧光激发光谱、荧光发射光谱、荧光量子产率,术语如Stokes位移和荧光影响因素等理论知识的理解,同时通过防伪应用开拓学生的思维,鼓励学生将课堂所学应用到实际生产生活中。

Chitosan conduits combined with nerve growth factor microspheres repair facial nerve defects

Microspheres containing nerve growth factor for sustained release were prepared by a compound method, and implanted into chitosan conduits to repair 10-mm defects on the right buccal branches of the facial nerve in rabbits. In addition, chitosan conduits combined with nerve growth factor or normal saline, as well as autologous nerve, were used as controls. At 90 days post-surgery, the muscular atrophy on the right upper lip was more evident in the nerve growth factor and normal sa- line groups than in the nerve growth factor-microspheres and autologous nerve groups. Electro- physiological analysis revealed that the nerve conduction velocity and amplitude were significantly higher in the nerve growth factor-microspheres and autologous nerve groups than in the nerve growth factor and normal saline groups. Moreover, histological observation illustrated that the di- ameter, number, alignment and myelin sheath thickness of myelinated nerves derived from rabbits were higher in the nerve growth factor-microspheres and autologous nerve groups than in the nerve growth factor and normal saline groups. These findings indicate that chitosan nerve conduits com- bined with microspheres for sustained release of nerve growth factor can significantly improve facial nerve defect repair in rabbits.

Consensus Control With a Constant Gain for Discrete-time Binary-valued Multi-agent Systems Based on a Projected Empirical Measure Method

This paper studies the consensus control of multiagent systems with binary-valued observations.An algorithm alternating estimation and control is proposed.Each agent estimates the states of its neighbors based on a projected empirical measure method for a holding time.Based on the estimates,each agent designs the consensus control with a constant gain at some skipping time.The states of the system are updated by the designed control,and the estimation and control design will be repeated.For the estimation,the projected empirical measure method is proposed for the binary-valued observations.The algorithm can ensure the uniform boundedness of the estimates and the mean square error of the estimation is proved to be at the order of the reciprocal of the holding time(the same order as that in the case of accurate outputs).For the consensus control,a constant gain is designed instead of the stochastic approximation based gain in the existing literature for binary-valued observations.And,there is no need to make modification for control since the uniform boundedness of the estimates ensures the uniform boundedness of the agents’states.Finally,the systems updated by the designed control are proved to achieve consensus and the consensus speed is faster than that in the existing literature.Simulations are given to demonstrate the theoretical results.

Promotional effects of Er incorporation in CeO_2(ZrO_2)/TiO_2 for selective catalytic reduction of NO by NH_3

A series CeO2（ZrO2）/TiO2 catalysts were modified with Er using a sol-gel method.The catalytic activity of the obtained catalysts in the selective catalytic reduction（SCR） of NO with NH3 was investigated to determine the appropriate Er dosage.The catalysts were characterized using X-ray diffraction,N2 adsorption,NH3 temperature-programmed desorption,H2 temperature-programmed reduction,photoluminescence spectroscopy,electron paramagnetic resonance spectroscopy,and X-ray photoelectron spectroscopy.The results showed that the optimum Er/Ce molar ratio was 0.10;this catalyst had excellent resistance to catalyst poisoning caused by vapor and sulfur and gave more than 90% NO conversion at 220–395 ℃ and a gas hourly space velocity of 71 400 h^-1.Er incorporation increased the Ti^3＋ concentrations,oxygen storage capacities,and oxygen vacancy concentrations of the catalysts,resulting in excellent catalytic performance.Er incorporation also decreased the acid strength and inhibited growth of TiO2 and CeO2 crystal particles,which increased the catalytic activity.The results show that high oxygen vacancy concentrations and oxygen storage capacities,large amounts of Ti^3＋,and low acid strengths give excellent SCR activity.

Endoscopic measurement of variceal diameter

AIM:To measure in vitro diameter of imitational varices using a self-made endoscopic scale and confirm its accuracy and clinical feasibility.METHODS:A catheter was introduced into the endoscopeaccessory channel and attached to a zebra wire guide that was used as a stylet.The wire guide was fixed onto the tip of the catheter by a soft and thin string.By gently advancing the stylet into the catheter,the width of the opening loop at the tip of the endoscope approximated the diameter of the imitational varices.Measurements performed in vitro using this self-made endoscopic ruler were compared to measurements of simulative varices.RESULTS:At the handle,the sleeve moving distance ranged from 5 to 14 mm.There was no obvious proportional relationship between the sleeve movement distance and endoscopic measurement ruler.The results indicated that the gap between the endoscopic measurement and actual measurement of the object size tended to close.The in vitro measurement of the diameter of the simulative varices showed that the two kinds of measuring methods were not significantly different with respect to their accuracy(P=0.8499).CONCLUSION:In vitro experiments confirmed that using a self-designed endoscopic ruler to measure the diameter of simulative varices was objective,accurate and feasible.

Electroacupuncture promotes the proliferation of endogenous neural stem cells and oligodendrocytes in the injured spinal cord of adult rats

A contusive model of spinal cord injury at spinal segment T8-9 was established in rats. Huantiao （GB30） and Huatuojiaji （Ex-B05） were punctured with needles, and endogenous neural stem cells were labeled with 5-bromo-2＇-deoxyuridine （BrdU） and NG2. Double immunofluorescence staining showed that electroacupuncture markedly increased the numbers of BrdU＋/NG2＋cells at spinal cord tissue 15 mm away from the injury center in the rostral and caudal directions. The results suggest that electroacupuncture promotes the proliferation of endogenous neural stem cells and oligodendrocytes in rats with spinal cord injury.

Influential nodes identification in complex networks based on global and local information

Identifying influential nodes in complex networks is essential for network robust and stability,such as viral marketing and information control.Various methods have been proposed to define the influence of nodes.In this paper,we comprehensively consider the global position and local structure to identify influential nodes.The number of iterations in the process of k-shell decomposition is taken into consideration,and the improved k-shell decomposition is then put forward.The improved k-shell decomposition and degree of target node are taken as the benchmark centrality,in addition,as is well known,the effect between node pairs is inversely proportional to the shortest path length between two nodes,and then we also consider the effect of neighbors on target node.To evaluate the performance of the proposed method,susceptible-infected(SI)model is adopted to simulate the spreading process in four real networks,and the experimental results show that the proposed method has obvious advantages over classical centrality measures in identifying influential nodes.