The correlation between microbiota plays a vital role in the progression of periodontal disease.This study investigated the in situ interaction networks between periodontal pathogens in periodontal and peri-implant di...The correlation between microbiota plays a vital role in the progression of periodontal disease.This study investigated the in situ interaction networks between periodontal pathogens in periodontal and peri-implant disease.We used quantitative real-time polymerase chain reaction and Pearson’s correlation coefficients to quantify the copy numbers and correlations of four oral core species—Fusobacterium nucleatum,Porphyromonas gingivalis,Prevotella intermedia,and Streptococcus gordonii—from 80 subgingival sites(healthy and with periodontitis or gingivitis)in patients with periodontitis,and 68 subgingival sites(healthy and with periodontitis,gingivitis,peri-implantitis,or peri-implant mucositis)in patients with implants.The highest bacterial counts were observed for Porphyromonas gingivalis and Prevotella intermedia at all the sites.Within the same cohorts,the bacterial loads were greater at diseased sites than at healthy sites.Bacterial counts did not differ among clinical sites in the same group(P>0.05)but differed between periodontitis and peri-implant mucositis sites in the two groups.Porphyromonas gingivalis,F.nucleatum,and Prevotella intermedia had strong correlations at gingivitis and healthy sites and moderate correlations at periodontitis sites in patients with periodontitis.In patients with implants,Prevotella intermedia,F.nucleatum,and S.gordonii had strong correlations only at peri-implantitis sites.Also,based on metagenomic analysis,F.nucleatum and Prevotella intermedia were significantly correlated at the subgingival plaque in peri-implantitis and periodontitis samples.Our results suggest that variations in microbe-microbe interactions in subgingival plaque reflect changes in the progression of periodontal disease,providing a new perspective for understanding the mechanisms of periodontitis and peri-implantitis.展开更多
目的建立复方气管炎片的微生物限度检查方法。方法分别采用平皿法及薄膜过滤法,加入金黄色葡萄球菌、铜绿假单胞菌、枯草芽孢杆菌、白色念珠菌、黑曲霉进行计数法适用性试验;将10 m L 1∶10供试液接种至不同体积的胰酪大豆胨液体培养基...目的建立复方气管炎片的微生物限度检查方法。方法分别采用平皿法及薄膜过滤法,加入金黄色葡萄球菌、铜绿假单胞菌、枯草芽孢杆菌、白色念珠菌、黑曲霉进行计数法适用性试验;将10 m L 1∶10供试液接种至不同体积的胰酪大豆胨液体培养基中,接种大肠埃希菌进行控制菌检查的适用性试验。结果需氧菌总数(TAMC)的计数采用平皿法(1∶20供试液)联用薄膜过滤法(1∶20供试液),霉菌和酵母菌总数(TYMC)的计数采用平皿法(1∶10供试液),各试验菌回收率均符合要求,控制菌检查采用培养基用量为500 m L的稀释法符合要求。结论联用平皿法和薄膜过滤法的微生物计数法可有效去除复方气管炎片中微生物的抑菌作用,有利于检出潜在污染微生物。展开更多
Through analysis of the connotation of unconventional hydrocarbons,the source-reservoir assemblages of unconventional hydrocarbons are divided into three types,i.e.,source-reservoir neighboring type,source-reservoir i...Through analysis of the connotation of unconventional hydrocarbons,the source-reservoir assemblages of unconventional hydrocarbons are divided into three types,i.e.,source-reservoir neighboring type,source-reservoir integration type,and source-reservoir separation type.The source-reservoir neighboring type resources mainly include tight oil and tight gas with the following formation conditions and characteristics:(1)efficient near-source accumulation close to high-quality source rocks with the pressure difference between source and reservoir as the dynamic force;(2)large-area tight reservoirs with certain porosity,dominated by micro-nanopores of inorganic origin;(3)distributed in depressions and slopes with large hydrocarbon-bearing area and great resource quantity,low abundance and local development of“sweet spots”;(4)weak tectonism.The source-reservoir integration type resources mainly include coalbed methane,shale gas,shale oil and oil shale.The formation conditions and characteristics are:(1)high abundance of organic matter,different types of organic matter and thermal evolution grades leading to formation of different resources types;(2)organic-rich sections are distributed continuously in the reservoiring space which is dominated by nanometer organic pores,interparticle pores and microfissures;(3)hydrocarbons mainly occur in free or adsorbed state,varying much with resource type;(4)the resources are self-conserved,and enriched in depressions and slopes,the conservation conditions are controlled by tectonics,temperature and pressure;(5)the production per well can be enhanced by volume fracturing,which increases the permeability of the reservoirs.The source-reservoir separation type resources are more special,mainly including(1)transformed conventional resources such as oil sand and heavy oil;(2)unsteady resources formed under special conditions of temperature and pressure,among which natural gas hydrate is a representative.These three types of resources in China are different in both development prospect and current situation:(1)the source-reservoir neighboring type is most practical and will develop fast in the next 5 years;(2)the source-reservoir integration type needs continuous exploration and technical breakthrough,and is expected to develop rapidly in the coming decade;(3)in the source-reservoir separation type resources,natural gas hydrates have the greatest development potential,on which technical tests are being conducted.展开更多
基金supported by grants from the Peking University School of Stomatology(PKUSS20170112).
文摘The correlation between microbiota plays a vital role in the progression of periodontal disease.This study investigated the in situ interaction networks between periodontal pathogens in periodontal and peri-implant disease.We used quantitative real-time polymerase chain reaction and Pearson’s correlation coefficients to quantify the copy numbers and correlations of four oral core species—Fusobacterium nucleatum,Porphyromonas gingivalis,Prevotella intermedia,and Streptococcus gordonii—from 80 subgingival sites(healthy and with periodontitis or gingivitis)in patients with periodontitis,and 68 subgingival sites(healthy and with periodontitis,gingivitis,peri-implantitis,or peri-implant mucositis)in patients with implants.The highest bacterial counts were observed for Porphyromonas gingivalis and Prevotella intermedia at all the sites.Within the same cohorts,the bacterial loads were greater at diseased sites than at healthy sites.Bacterial counts did not differ among clinical sites in the same group(P>0.05)but differed between periodontitis and peri-implant mucositis sites in the two groups.Porphyromonas gingivalis,F.nucleatum,and Prevotella intermedia had strong correlations at gingivitis and healthy sites and moderate correlations at periodontitis sites in patients with periodontitis.In patients with implants,Prevotella intermedia,F.nucleatum,and S.gordonii had strong correlations only at peri-implantitis sites.Also,based on metagenomic analysis,F.nucleatum and Prevotella intermedia were significantly correlated at the subgingival plaque in peri-implantitis and periodontitis samples.Our results suggest that variations in microbe-microbe interactions in subgingival plaque reflect changes in the progression of periodontal disease,providing a new perspective for understanding the mechanisms of periodontitis and peri-implantitis.
文摘目的建立复方气管炎片的微生物限度检查方法。方法分别采用平皿法及薄膜过滤法,加入金黄色葡萄球菌、铜绿假单胞菌、枯草芽孢杆菌、白色念珠菌、黑曲霉进行计数法适用性试验;将10 m L 1∶10供试液接种至不同体积的胰酪大豆胨液体培养基中,接种大肠埃希菌进行控制菌检查的适用性试验。结果需氧菌总数(TAMC)的计数采用平皿法(1∶20供试液)联用薄膜过滤法(1∶20供试液),霉菌和酵母菌总数(TYMC)的计数采用平皿法(1∶10供试液),各试验菌回收率均符合要求,控制菌检查采用培养基用量为500 m L的稀释法符合要求。结论联用平皿法和薄膜过滤法的微生物计数法可有效去除复方气管炎片中微生物的抑菌作用,有利于检出潜在污染微生物。
基金This work was funded by National Science and Technology Major Project of China(Grant No.2011ZX05043)Major Science and Technology Special Project of China National Petroleum Corporation(Grant No:2013E-0502)。
文摘Through analysis of the connotation of unconventional hydrocarbons,the source-reservoir assemblages of unconventional hydrocarbons are divided into three types,i.e.,source-reservoir neighboring type,source-reservoir integration type,and source-reservoir separation type.The source-reservoir neighboring type resources mainly include tight oil and tight gas with the following formation conditions and characteristics:(1)efficient near-source accumulation close to high-quality source rocks with the pressure difference between source and reservoir as the dynamic force;(2)large-area tight reservoirs with certain porosity,dominated by micro-nanopores of inorganic origin;(3)distributed in depressions and slopes with large hydrocarbon-bearing area and great resource quantity,low abundance and local development of“sweet spots”;(4)weak tectonism.The source-reservoir integration type resources mainly include coalbed methane,shale gas,shale oil and oil shale.The formation conditions and characteristics are:(1)high abundance of organic matter,different types of organic matter and thermal evolution grades leading to formation of different resources types;(2)organic-rich sections are distributed continuously in the reservoiring space which is dominated by nanometer organic pores,interparticle pores and microfissures;(3)hydrocarbons mainly occur in free or adsorbed state,varying much with resource type;(4)the resources are self-conserved,and enriched in depressions and slopes,the conservation conditions are controlled by tectonics,temperature and pressure;(5)the production per well can be enhanced by volume fracturing,which increases the permeability of the reservoirs.The source-reservoir separation type resources are more special,mainly including(1)transformed conventional resources such as oil sand and heavy oil;(2)unsteady resources formed under special conditions of temperature and pressure,among which natural gas hydrate is a representative.These three types of resources in China are different in both development prospect and current situation:(1)the source-reservoir neighboring type is most practical and will develop fast in the next 5 years;(2)the source-reservoir integration type needs continuous exploration and technical breakthrough,and is expected to develop rapidly in the coming decade;(3)in the source-reservoir separation type resources,natural gas hydrates have the greatest development potential,on which technical tests are being conducted.
