The DNA damage repair complex MoMMS21-MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae

The conserved DNA damage repair complex,MMS21-SMC5/6(Methyl methane sulfonate 21-Structural maintenance of chromosomes 5/6),has been extensively studied in yeast,animals,and plants.However,its role in phytopathogenic fungi,particularly in the highly destructive rice blast fungus Magnaporthe oryzae,remains unknown.In this study,we functionally characterized the homologues of this complex,MoMMS21 and MoSMC5,in M.oryzae.We first demonstrated the importance of DNA damage repair in M.oryzae by showing that the DNA damage inducer phleomycin inhibited vegetative growth,infection-related development and pathogenicity in this fungus.Additionally,we discovered that MoMMS21 and MoSMC5 interacted in the nuclei,suggesting that they also function as a complex in M.oryzae.Gene deletion experiments revealed that both MoMMS21 and MoSMC5 are required for infection-related development and pathogenicity in M.oryzae,while only MoMMS21 deletion affected growth and sensitivity to phleomycin,indicating its specific involvement in DNA damage repair.Overall,our results provide insights into the roles of MoMMS21 and MoSMC5 in M.oryzae,highlighting their functions beyond DNA damage repair.

Trehalose attenuates abdominal aortic aneurysm formation by inducing autophagy in smooth muscle cells

BACKGROUND Trehalose is a naturally occurring disaccharide,which has been identified as an autophagy inducer and ex-hibits protective effect in cardiovascular diseases such as myocardial infraction and atherosclerosis.However,the functional role of trehalose in abdominal aortic aneurysm(AAA)remains undefined.METHODS To study the effect of trehalose in AAA,trehalose(1 g/kg per day)were given for 14 continuous days in a mouse model of elastase-induced abdominal aortic aneurysm.On day 14,ultrasound was performed to measure aortic diameter before the abdominal aortas were harvested and processed for further analysis.Verhoeff-Van Gieson staining and TUNEL staining were performed on paraffin sections to evaluate vascular histology and apoptosis,immunofluorescence staining and Western-blot were performed to evaluate expression of autophagy markers.RESULTS Echocardiography and in situ pictures demonstrated that trehalose attenuated infrarenal aorta dilation.Verhoeff-Van Gieson staining showed elastin degradation was improved in trehalose-treated group.Compared with vehicle-treated mice,trehalose treatment restored smooth muscle cell contractile phenotype with increasedα-SMA,Calponin and Myh11 expression.Furthermore,trehalose also attenuated cell apoptosis and leukocytes infiltration.Importantly,trehalose induced autophagy with decrease SQSTM1/p62 accumulation,increased lamp2 expression and LC3B conversion.CONCLUSION Trehalose attenuated AAA progression with decreased inflammation and restored SMC contractile phenotype by inducing autophagy.These results demonstrated the therapeutic potential of trehalose in AAA.

Light-absorbing Particles in Snow and Ice: Measurement and Modeling of Climatic and Hydrological impact

Light absorbing particles（LAP, e.g., black carbon, brown carbon, and dust） influence water and energy budgets of the atmosphere and snowpack in multiple ways. In addition to their effects associated with atmospheric heating by absorption of solar radiation and interactions with clouds, LAP in snow on land and ice can reduce the surface reflectance（a.k.a., surface darkening）, which is likely to accelerate the snow aging process and further reduces snow albedo and increases the speed of snowpack melt. LAP in snow and ice（LAPSI） has been identified as one of major forcings affecting climate change, e.g.in the fourth and fifth assessment reports of IPCC. However, the uncertainty level in quantifying this effect remains very high. In this review paper, we document various technical methods of measuring LAPSI and review the progress made in measuring the LAPSI in Arctic, Tibetan Plateau and other mid-latitude regions. We also report the progress in modeling the mass concentrations, albedo reduction, radiative forcing, and climatic and hydrological impact of LAPSI at global and regional scales. Finally we identify some research needs for reducing the uncertainties in the impact of LAPSI on global and regional climate and the hydrological cycle.

TIN_DDM Buffer Surface Construction Algorithm Based on Rolling Ball Acceleration Optimization Model

In view of the TIN_DDM buffer surface existing in the construction and application of special data type,algorithm efficiency and precision are not matching;the paper applied the rolling ball model in the process of TIN_DDM buffer surface construction.Based on the precision limitation analysis of rolling ball model,the overall precision control method of rolling ball model has been established.Considering the efficiency requirement of TIN_DDM buffer surface construction,the influence principle of key sampling points and rolling ball radius to TIN_DDM buffer surface construction efficiency has been elaborated,and the rule of identifying key sampling points has also been designed.Afterwards,by erecting the numerical relationship between key sampling points and rolling ball radius,a TIN_DDM buffer surface construction algorithm based on rolling ball acceleration optimization model has been brought forward.The time complexity of the algorithm is O(n).The experiments show that the algorithm could realize the TIN_DDM buffer surface construction with high efficiency,and the algorithm precision is controlled with in 2σ.

Construction of a chromosome-level genome assembly for genome-wide identification of growth-related quantitative trait loci in Sinocyclocheilus grahami(Cypriniformes,Cyprinidae)

The Dianchi golden-line barbel,Sinocyclocheilus grahami(Regan,1904),is one of the“Four Famous Fishes”of Yunnan Province,China.Given its economic value,this species has been artificially bred successfully since 2007,with a nationally selected breed(“S.grahami,Bayou No.1”)certified in 2018.For the future utilization of this species,its growth rate,disease resistance,and wild adaptability need to be improved,which could be achieved with the help of molecular markerassisted selection(MAS).In the current study,we constructed the first chromosome-level genome of S.grahami,assembled 48 pseudo-chromosomes,and obtained a genome size of 1.49 Gb.We also performed QTL-seq analysis of S.grahami using the highest and lowest bulks(i.e.,largest and smallest size)in both a sibling and random population.We screened two quantitative trait loci(QTLs)(Chr3,14.9-39.1 Mb and Chr17,4.1-27.4 Mb)as major growth-related locations.Several candidate genes(e.g.,map2k5,stat1,phf21a,sox6,and smad6)were also identified,with functions related to growth,such as cell differentiation,neuronal development,skeletal muscle development,chondrogenesis,and immunity.These results built a solid foundation for in-depth MAS studies on the growth traits of S.grahami.

The Study on Urban Agglomeration Environmental Rights Trading Paradigm—With the Emission Trading Model of Wan-Jiang City Belt

As the deterioration of the modern environmental problems, developed countries started to explore concrete ways of internalization of external effects produced by environmental problems. The theory of new institutional economics which produced the environmental property theory is widely used in the research of environmental management. On the premise of total amount control of pollution, the subjects of public power distribute or sell the rights is the basic paradigm of this method. Buyers take possession of the rights and utilize, profit from or dispose of them. The coordinated development of urban agglomeration is the main direction of China’s regional development strategy. As there are differences in environmental governance in different cities, the environment property trading system of urban agglomeration needs to be established urgently. Due to environmental property’s complexity and its attributes of public goods, the transaction of environmental property is restricted by amounts of factors. This research concludes that it is necessary to define the possessor of environmental property, strengthen the construction of trading platform, improving the public participation and supervision mechanism and control transaction cost by establishing and analyzing the emission trading model of the city-cluster along the Yangtze River in Anhui Province.

National Innovation-Oriented City Evaluation Study Based on Two-Stage DEA Model

The research on the evaluation indicator system of innovation-oriented cities has a long history. It presents different characteristics in different countries or regions. In 2006, China proposed the national strategy to build an innovation-oriented nation and subsequently approved more than 60 pilot cities in the country. The research on China’s innovation-oriented city assessment system is also increasing. On the basis of predecessors, this paper constructs innovation-oriented city evaluation indicator system with more links based on innovation link. It has three categories with 44 indicators in total according to input, intermediate output and final output. It creatively introduces in two-stage DEA model on sharing input and constructs the evaluation model based on the above evaluation indicator system. This paper makes an empirical analysis on the innovation-oriented cities in Yangtze River Delta including Hefei, arrives at innovation efficiency difference and puts forward some countermeasures and suggestions for the analysis results.

Rice ferredoxin OsFd4 contributes to oxidative stress tolerance but compromises defense against blight bacteria

Ferredoxins(Fds)in plastids are the most upstream stromal electron receptors shuttling electrons to downstream metabolic systems and function in various physiological processes of dicots,but their roles in monocots’response to stresses are still unclear.In this study,the functions of OsFd4,the major non-photosynthetic type Fd in rice,were characterized under oxidative stress and Xanthomonas oryzae pv.oryzae(Xoo)infection.OsFd4-knockout mutants displayed no defects in key agronomic traits and blast resistance,but were more sensitive to hydrogen peroxide(H2O2)treatment than the wild type.Transient expression of OsFd4 alleviated H2O2-induced rice cell death,suggesting that OsFd4 contributes to rice tolerance to exogenous oxidative stress.Deletion of OsFd4 enhanced rice immune responses against Xoo.OsFd4 formed a complex in vivo with itself and OsFd1,the major photosynthetic Fd in rice,and OsFd1 transcripts were increased in leaf and root tissues of the OsFd4-knockout mutants.These results indicate that OsFd4 is involved in regulating rice defense against stresses and interplays with OsFd1.

大数据时代背景下幼儿园教师培训体系的重构

培训是促进幼儿园教师专业发展的重要手段,高质量的教师培训可以有效提升教师的专业素质和幼儿园的教育质量。随着大数据时代的发展和幼儿园教育情境的日趋多样化与复杂化,传统培训愈发难以满足教师专业发展的需要。在此时代背景下重构幼儿园教师培训体系,就是要利用大数据准确了解幼儿园教师的培训需求,使培训更为精准化;利用大数据构建幼儿园教师培训资源库,满足教师专业学习的多样化需求;利用大数据构建合理的教师培训方案和培训课程,实行分层培训,提高培训的适切性和有效性。

Yunnanilus chuanheensis, a new loach species(Cypriniformes: Nemacheilidae) from the upper Lixianjiang River in Yunnan, China

DEAR EDITOR,A new loach species(Cypriniformes: Nemacheilidae:Yunnanilini), Yunnanilus chuanheensis sp. nov., was caught from Chuanhe in the upper reaches of the Lixianjiang River, a tributary of the Red River in Nanjian County, Yunnan Province, China. This species is a member of the traditional Y.pleurotaenia species group based on the presence of a lateral line and cephalic lateral-line canals.

老龄化背景下的社区照顾模式问题探究

随着我国人口老龄化程度日益加剧,老年人的养老问题成为了社会关注的热点问题。目前,社区照顾模式已成为了解决我国老人养老问题的主要途径之一。社区照顾模式存在着部分居民对社区养老模式认识不足、照顾供给和需求存在不匹配现象、专业人员缺乏志愿者队伍不壮大、法律法规不完善问题,针对存在的问题提出了相应的完善措施。

Risk factors for renal outcomes in children with antineutrophil cytoplasmic antibody-associated vasculitis:a nationwide retrospective study in China

Background Pediatric antineutrophil cytoplasmic antibody-associated vasculitis(AAV)is a life-threatening systemic vasculitis featured by liability to renal involvement.However,there are few studies on the risk factors and predictive models for renal outcomes of AAV in children.Methods Data from 179 AAV children in multiple centers between January 2012 and March 2020 were collected retrospectively.The risk factors and predictive model of end-stage renal disease(ESRD)in AAV were explored.Results Renal involvement was the most typical manifestation(95.5%),and the crescent was the predominant pathological lesion(84.9%).The estimated glomerular filtration rate(eGFR)was evaluated in 114 patients,of whom 59.6%developed ESRD,and the median time to ESRD was 3.20 months.The eGFR[P=0.006,odds ratio(OR)=0.955,95%confidence interval(CI)=0.924–0.987]and the percentages of global glomerulosclerosis(pGGS;P=0.018,OR=1.060,95%CI=1.010–1.112)were independent risk factors for ESRD of renal biopsy.Based on the pGGS and eGFR at renal biopsy,we developed three risk grades of ESRD and one predictive model.The Kaplan‒Meier curve indicated that renal outcomes were significantly different in different risk grades(P<0.001).Compared with serum creatinine at baseline,the predictive model had higher accuracy(0.86 versus 0.58,P<0.001)and a lower coefficient of variation(0.07 versus 0.92)in external validation.Conclusions Renal involvement is the most common manifestation of pediatric AAV in China,of which more than half deteriorates into ESRD.The predictive model based on eGFR at renal biopsy and the pGGS may be stable and accurate in speculating the risk of ESRD in AAV children.

A genomic association study revealing subphenotypes of childhood steroid-sensitive nephrotic syndrome in a larger genomic sequencing cohort

Dissecting the genetic components that contribute to the two main subphenotypes of steroid-sensitive nephrotic syndrome(SSNS)using genome-wide association studies(GWAS)strategy is important for understanding the disease.We conducted a multicenter cohort study(360 patients and 1835 controls)combined with a GWAS strategy to identify susceptibility var-iants associated with the following two subphenotypes of ssNS:steroid-sensitive nephrotic syn-drome without relapse(SSNswR,181 patients)and steroid-dependent/frequent relapse nephrotic syndrome(SDNS/FRNS,179 patients).The distribution of two single-nucleotide poly-morphisms(SNPs)in ANKRD36 and ALPG was significant between SSNSWR and healthy controls,and that of two SNPs in GAD1 and HLA-DQA1 was significant between SDNS/FRNS and healthy controls.Interestingly,rs1047989 in HLA-DQA1 was a candidate locus for SDNS/FRNS but not for SSNSWR.No significant SNPs were observed between SSNSWR and SDNS/FRNS.Meanwhile,chromosome 2:171713702 in GAD1 was associated with a greater steroid dose(>0.75 mg/kg/d)upon relapse to first remission in patients with SDNS/FRNS(odds ratio=3.14;95%confidence interval,0.97-9.87;P=0.034).rs117014418 in APOL4 was significantly associated with a decrease in eGFR of greater than 20%compared with the baseline in SDNS/FRNS patients(P=0.0001).Protein-protein intersection network construction suggested that HLA-DQA1 and HLA-DQB1 function together through GSDMA.Thus,SSNSWR belongs to non-HLA region-dependent nephropathy,and the HLA-DQA/DQB region is likely strongly associated with dis-ease relapse,especially in SDNS/FRNS.The study provides a novel approach for the GWAS strategy of SsNS and contributes to our understanding of the pathological mechanisms of SSNSWRandSDNS/FRNS.

Utility of MtCOI polymerase chain reaction-restriction fragment length polymorphism in differentiating between Q and B whitefly Bemisia tabaci biotypes

The invasive, insecticide-resistant, Q whitefly biotype, has gradually spread to other countries including the US via human-mediated movement of plant materials. We assessed the utility of the VspI-based mtCOI （mitochondrion cytochrome oxidase I） polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique as a rapid, cost-effective, and reliable alternative for differentiating the Q from the dominant B biotype in Arizona. Using the standard mtCOI gene sequencing and mtCOI PCR-RFLP techniques, we biotyped eight whitefly strains of five individuals each collected from poinsettia and cotton at different locations in Arizona. Complete concordance was observed between the two methods, with three strains being identified as the Q biotype and five samples as the B biotype. We also scanned the mtCOI gene sequences for VspI polymorphisms in the B and Q biotype whiteflies currently available in the GenBank database. This global screening revealed the existence of three and four VspI polymorphic types for the Q and B biotypes, respectively. Nevertheless, all three VspI polymorphic Q biotype whiteflies shared a common and unique VspI site that can be used to differentiate Q biotype from the four VspI polymorphic B biotype whiteflies identified. These results demonstrate that the VspI-based mtCOI gene PCR-RFLP provides a reliable diagnostic tool for differentiating the Q and B biotype whiteflies in the US and elsewhere.

路易斯碱催化的不对称C-酰化反应

不对称C-酰化反应是便捷构建季碳手性中心最有效的方法之一.不对称C-酰化反应可分为间接不对称C-酰化与直接不对称C-酰化.路易斯碱催化剂在这类反应中起到了关键性作用.作用于C-酰化反应的路易斯碱催化剂可分为叔胺类、叔膦类和亲核氧类.其中叔胺类应用最广泛并且结构最多样,主要包括氨基吡啶、脒和咪唑三类结构.本文梳理了近年来路易斯碱催化不对称C-酰化反应的发展历程,尤其详细介绍了手性双环咪唑催化的直接不对称C-酰化反应,着重探讨了催化剂在这些反应中的催化性能和作用机理.

基于系留气球的藏东南大气黑碳垂直分布

在青藏高原东南部地区开展大气黑碳垂直分布的探测对研究黑碳跨境传输过程及其气候环境影响具有重要的意义.本研究利用系留气球观测平台获得青藏高原大气黑碳浓度的垂直廓线.观测结果显示,稳定大气边界层黑碳浓度为1000±750 ng/m^3,生物燃烧源贡献为44%±37%;剩余层黑碳浓度为140±50 ng/m^3,生物燃烧源贡献为16%±6%;自由大气黑碳浓度为120±50 ng/m3,生物燃烧源贡献为14%±5%.此外,本项研究表明稳定大气边界层和剩余层大气黑碳主要来源于局地排放,而自由大气黑碳则主要受西风急流长距离传输的影响.

水稻稻瘟病抗性基因的克隆、育种利用及稻瘟菌无毒基因研究进展

稻瘟病是世界上影响水稻(Oryza sativa)粮食生产的主要病害之一,抗病基因的发掘与利用是抗病育种的基础和核心。随着寄主水稻和病原菌稻瘟病菌(Magnaporthe oryzae)基因组测序和基因注释的完成,水稻和稻瘟病菌的互作体系成为研究植物与真菌互作的模式系统。该文对稻瘟病抗病基因的遗传、定位、克隆及育种利用进行概述,并通过生物信息学分析方法,探讨了水稻全基因组中NBS-LRR类抗病基因在水稻12条染色体上的分布情况,同时对稻瘟病菌无毒基因的鉴定及无毒蛋白与抗病蛋白的互作进行初步分析。最后对稻瘟病抗病基因研究存在的问题进行分析并展望了未来的研究方向,以期为水稻抗稻瘟病育种发展和抗病机制的深入理解提供参考。

The Role of OsMSH5 in Crossover Formation during Rice Meiosis

MSH5, a meiosis-specific member of the MutS-homolog family, is required for normal level of recombination in budding yeast, mice, Caenorhabditis elegans, and Arabidopsis. Here, we report the identification and characterization of its rice homolog, OsMSH5, and demonstrate its function in rice meiosis. Five independent Osmsh5 mutants exhibited normal vegetative growth and severe sterility. The synaptonemal complex is well installed in Osmsh5, while the chiasma frequency is greatly reduced to approximately 10% of that observed in the wild-type, leading to the homologous non- disjunction and complete sterile phenotype. OsMSH5 is predominantly expressed in panicles. Immunofluorescence studies indicate that OsMSH5 chromosomal localization is limited to the early meiotic prophase I. OsMSH5 can be loaded onto meiotic chromosomes in Oszip4, Osmer3, and hellO. However, those ZMM proteins cannot be localized normally in the absence of OsMSH5. Furthermore, the residual chiasmata were shown to be the least frequent among the zmm mutants, including Osmer3, Oszip4, hellO, and Osmsh5. Taken together, we propose that OsMSH5 functions upstream of OsZIP4, OsMER3, and HEIl0 in class I crossover formation.

Application of Omega vortex identification method in cavity buffeting noise

The cavity buffeting noise is related to the free shear layer oscillation and the periodic vortex shedding,where weak vortices coexist with strong vortices and the strong shear phenomenon also exists at the opening of the cavity.Therefore,it is of great significance to accurately capture vortices at the opening for the control of the cavity buffeting noise.This paper first compares the Omega vortex identification method with the Q andλ2criteria based on the large eddy simulation(LES)of the backward-facing step flow,and it is found that the Omega method enjoys the following advantages:it is not sensitive to a moderate threshold change andΩ=0.52 can be used as a fixed threshold,it can capture both the strong and weak vortices at the same time;and it will not be contaminated by the shear.Then the Omega(Ω)method is applied to the LES of the cavity buffeting noise:the mechanism of the cavity buffeting noise is studied based on a simple cavity model firstly,and then the effects of the incoming boundary layer thicknesses and the incoming boundary layer shapes on the cavity buffeting noise are analyzed.The results show that:theΩmethod clearly captures the processes of the vortex generation,development,collision and fragmentation,verifying that the generation of the cavity buffeting noise is related to the free shear layer oscillation and the periodic vortex shedding;as the thickness of the incoming boundary layer increases,the free shear layer becomes more stable and the Helmholtz resonance is avoided effectively,thereby the cavity buffeting noise is reduced effectively,adding a convexity upstream of the cavity opening to interfere the shape of the incoming boundary layer to reduce the acoustic feedback effect can reduce the cavity buffeting noise effectively.

SH3P2,an SH3 domain-containing protein that interacts with both Pib and AvrPib,suppresses effector-triggered,Pib-mediated immunity in rice

Plants usually keep resistance(R)proteins in a static state under normal conditions to avoid autoimmunity and save energy for growth,but R proteins can be rapidly activated upon perceiving pathogen invasion.Pib,the first cloned blast disease R gene in rice,encoding a nucleotide-binding leucine-rich repeat(NLR)protein,mediates resistance to the blast fungal(Magnaporthe oryzae)isolates carrying the avirulence gene AvrPib.However,the molecular mechanisms about how Pib recognizes AvrPib and how it is inactivated and activated remain largely unclear.In this study,through map-based cloning and CRISPR-Cas9 gene editing,we proved that Pib contributes to the blast disease resistance of rice cultivar Yunyin(YY).Furthermore,an SH3 domain-containing protein,SH3P2,was found to associate with Pib mainly at clathrin-coated vesicles in rice cells,via direct binding with the coiled-coil(CC)domain of Pib.Interestingly,overexpression of SH3P2 in YY compromised Pib-mediated resistance to M.oryzae isolates carrying AvrPib and Pib-AvrPib recognition-induced cell death.SH3P2 competitively inhibits the self-association of the Pib CC domain in vitro,suggesting that binding of SH3P2 with Pib undermines its homodimerization.Moreover,SH3P2 can also interact with AvrPib and displays higher affinity to AvrPib than to Pib,which leads to dissociation of SH3P2 from Pib in the presence of AvrPib.Taken together,our results suggest that SH3P2 functions as a“protector”to keep Pib in a static state by direct interaction during normal growth but could be triggered off by the invasion of AvrPib-carrying M.oryzae isolates.Our study reveals a new mechanism about how an NLR protein is inactivated under normal conditions but is activated upon pathogen infection.