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ShRNA-LDH纳米颗粒的制备及薇甘菊生物防治 被引量:1
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作者 莫亦琳 陈伟钊 +4 位作者 黄丽娟 吴飞燕 肖念 于宇 刘学东 《深圳大学学报(理工版)》 CAS CSCD 北大核心 2023年第2期163-170,共8页
核糖核酸干扰(ribonucleic acid interference,RNAi)技术在植物保护中具有高效、安全和环保的特点.外源喷施短发夹RNA(short hairpin RNA,shRNA)可以有效沉默薇甘菊(Mikania micrantha)叶绿素a/叶绿素b结合蛋白相关基因.为提高喷施shRN... 核糖核酸干扰(ribonucleic acid interference,RNAi)技术在植物保护中具有高效、安全和环保的特点.外源喷施短发夹RNA(short hairpin RNA,shRNA)可以有效沉默薇甘菊(Mikania micrantha)叶绿素a/叶绿素b结合蛋白相关基因.为提高喷施shRNA对薇甘菊的防治效果,制备直径为10~100 nm的层状双氢氧化物(layered double hydroxide,LDH)黏土纳米片为shRNA的载体,该载体具有良好的生物相容性,具环境友好.研究发现,LDH结合shRNA的最佳质量比为1∶10.shRNA喷施实验表明,有轻微创伤的薇甘菊叶片对shRNA更敏感,并且LDH直接喷施对薇甘菊的生长几乎没有任何影响.为比较LDH-shRNA和单独的shRNA对薇甘菊的控制效果,利用分别搭载了6个shRNA的LDH纳米颗粒或单独的shRNA喷施薇甘菊叶片,在喷施6 d后观察到叶片喷施LDH-shRNA有更加明显的坏死表型.研发的基于LDH的shRNA携带系统可显著提高RNAi在防控薇甘菊中的有效性. 展开更多
关键词 核糖核酸干扰 植物保护 薇甘菊 叶绿素a/b结合蛋白 黏土纳米片 短发夹核糖核酸
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Security for cyber-physical systems: Secure control against known-plaintext attack 被引量:1
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作者 YUAN Ye mo yilin 《Science China(Technological Sciences)》 SCIE EI CAS CSCD 2020年第9期1637-1646,共10页
There has been a surge of interests in the security of cyber-physical systems(CPSs), yet it is commonly assumed that the adversary has a full knowledge of physical system models. This paper argues that such an unreali... There has been a surge of interests in the security of cyber-physical systems(CPSs), yet it is commonly assumed that the adversary has a full knowledge of physical system models. This paper argues that such an unrealistic assumption can be relaxed: the adversary might still be able to identify the system model by passively observing the control input and sensory data. In such a setup, the attack with knowledge of input-output data can be categorized as a Known-Plaintext Attack. A necessary and sufficient condition has been provided, under which the adversary can uniquely obtain the knowledge of the underlying physical system.From the defender's perspective, a secure controller design—which exhibits a low rank structure—is proposed which renders the system unidentifiable to the adversary, while trading off the control system's performance. Finally, a numerical example has been provided to demonstrate the effectiveness of the proposed secure controller design. 展开更多
关键词 cyber-physical systems SECURITY secure control known-plaintext attack
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