The anticoagulant effect of leech saliva was traditionally employed in the treatment of diabetes mellitus complications such as peripheral vascular complications.This study was carried out to examine the effect of lee...The anticoagulant effect of leech saliva was traditionally employed in the treatment of diabetes mellitus complications such as peripheral vascular complications.This study was carried out to examine the effect of leech saliva extract(LSE)on blood glucose levels in alloxan-induced diabetic rats.First,LSE was collected from leeches which were fed on a phagostimulatory solution.Second,total protein concentration was estimated using the Bradford assay.Third,diabetic rats were injected subcutaneously(sc)with LSE at doses of 500 and 1 000μg·kg 1body weight(bw).Other diabetic rats were injected sc with insulin at doses of 10 and 20 U·kg 1bw.Another group was injected simultaneously with LSE(250μg·kg 1bw)and insulin(10 U·kg 1bw).Fasting blood glucose(FBG)concentrations were monitored during a study period of eight hours at regular intervals.Findings showed that both doses of LSE resulted in a significant and gradual decrease in FBG starting from 10%18%downfall after two hours of injection reaching the maximal reduction activity of 58%after eight hours.Remarkably,LSE was sufficient to bring the rats to a near norm-glycemic state.The high dose of insulin induced a severe hypoglycemic condition after 2 4 h of injection.The lower dose was able to decline FBG for 2 6 h in rats which became diabetic again after 8 h.On the other hand,the concurrent injection of low doses of LSE and insulin produced a hypoglycemic effect with all rats showing normal FBG levels.Taken together,these findings indicated that the subcutaneous injection of LSE of the medicinal Malaysian leech was able to provide better glycemic control compared with insulin.Moreover,the synergism between LSE and insulin suggests that LSE could be utilized as an adjuvant medication in order to reduce insulin dosage or to achieve better control of blood glucose.展开更多
Designed and synthesized a new highly water soluble N,N^1-bis（2-（（5-（（dimethylamino）methyl）furan-2-yl）methylthio）ethyl）-perylene -3,4,9,10-tetracarboxylic diimide from 2-（（5-（（dimethylamino）methyl）fura...Designed and synthesized a new highly water soluble N,N^1-bis（2-（（5-（（dimethylamino）methyl）furan-2-yl）methylthio）ethyl）-perylene -3,4,9,10-tetracarboxylic diimide from 2-（（5-（（dimethylamino）methyl）furan-2-yl）methylthio）ethanamine and perylene-3,4,9,10- tetracarboxylic dianhydride.The compound was characterized by ~1H,^（13）C,2D NMR,mass and IR techniques.The compound is highly fluorescent with good solubility in water and other polar solvents.展开更多
Some new pyridazinone derivatives(3) were synthesized through addition of antipyrin to β- aroylacrylic acids (1) followed by cyclization of the adducts. Reactions of 3 with ethyl bromoacetate, benzenesulfonyl chlorid...Some new pyridazinone derivatives(3) were synthesized through addition of antipyrin to β- aroylacrylic acids (1) followed by cyclization of the adducts. Reactions of 3 with ethyl bromoacetate, benzenesulfonyl chloride, benzoyl chloride and POCl_3 give N-substituted products and monochlorosub- stituted pyridazines (4), respectively. The reactions of the latter with thiourea give unexpected dithio- derivatives(5), which react with phenylhydrazine to give pyrazolopyridazino pyridazine derivatives (6). Reactions of 2 with NH_2OH in ethanol and pyridine afford oxime and oxazinone derivatives 7 and 8, tespectively. The antibacterial activities of several compounds were screened.展开更多
基金supported by the grant(No.EDW B 10-0393)from Research-Management Centre,International Islamic University Malaysia
文摘The anticoagulant effect of leech saliva was traditionally employed in the treatment of diabetes mellitus complications such as peripheral vascular complications.This study was carried out to examine the effect of leech saliva extract(LSE)on blood glucose levels in alloxan-induced diabetic rats.First,LSE was collected from leeches which were fed on a phagostimulatory solution.Second,total protein concentration was estimated using the Bradford assay.Third,diabetic rats were injected subcutaneously(sc)with LSE at doses of 500 and 1 000μg·kg 1body weight(bw).Other diabetic rats were injected sc with insulin at doses of 10 and 20 U·kg 1bw.Another group was injected simultaneously with LSE(250μg·kg 1bw)and insulin(10 U·kg 1bw).Fasting blood glucose(FBG)concentrations were monitored during a study period of eight hours at regular intervals.Findings showed that both doses of LSE resulted in a significant and gradual decrease in FBG starting from 10%18%downfall after two hours of injection reaching the maximal reduction activity of 58%after eight hours.Remarkably,LSE was sufficient to bring the rats to a near norm-glycemic state.The high dose of insulin induced a severe hypoglycemic condition after 2 4 h of injection.The lower dose was able to decline FBG for 2 6 h in rats which became diabetic again after 8 h.On the other hand,the concurrent injection of low doses of LSE and insulin produced a hypoglycemic effect with all rats showing normal FBG levels.Taken together,these findings indicated that the subcutaneous injection of LSE of the medicinal Malaysian leech was able to provide better glycemic control compared with insulin.Moreover,the synergism between LSE and insulin suggests that LSE could be utilized as an adjuvant medication in order to reduce insulin dosage or to achieve better control of blood glucose.
文摘Designed and synthesized a new highly water soluble N,N^1-bis（2-（（5-（（dimethylamino）methyl）furan-2-yl）methylthio）ethyl）-perylene -3,4,9,10-tetracarboxylic diimide from 2-（（5-（（dimethylamino）methyl）furan-2-yl）methylthio）ethanamine and perylene-3,4,9,10- tetracarboxylic dianhydride.The compound was characterized by ~1H,^（13）C,2D NMR,mass and IR techniques.The compound is highly fluorescent with good solubility in water and other polar solvents.
文摘Some new pyridazinone derivatives(3) were synthesized through addition of antipyrin to β- aroylacrylic acids (1) followed by cyclization of the adducts. Reactions of 3 with ethyl bromoacetate, benzenesulfonyl chloride, benzoyl chloride and POCl_3 give N-substituted products and monochlorosub- stituted pyridazines (4), respectively. The reactions of the latter with thiourea give unexpected dithio- derivatives(5), which react with phenylhydrazine to give pyrazolopyridazino pyridazine derivatives (6). Reactions of 2 with NH_2OH in ethanol and pyridine afford oxime and oxazinone derivatives 7 and 8, tespectively. The antibacterial activities of several compounds were screened.
