Diabetes is usually associated with cerebrovascular disease,especially stroke.In practice,fasudil is widely accepted to be applied for the treatment of vascular disease.This article demonstrates the study concentratin...Diabetes is usually associated with cerebrovascular disease,especially stroke.In practice,fasudil is widely accepted to be applied for the treatment of vascular disease.This article demonstrates the study concentrating on the effects of fasudil pretreatment on the prognosis of diabetic stroke.250—300 g SpragueDawley rats were randomly divided into three groups,non-diabetic stroke group,diabetic stroke group,and fasudil pretreatment group.The rats of diabetes group were treated with intraperitoneal injection of streptozotocin(60 mg/kg),in the meantime the same dose of citrate buffer was injected into those of the control group.The rats of the fasudil group received daily fasudil intraperitoneal injection at 10 mg/kg for three consecutive weeks.After four weeks,all the rats of the experimental group were treated with middle cerebral artery occlusion for 90 min.After sacrifice,the fresh brain samples were collected for following experiments,including infarct volume,edema volume,blood-brain barrier(BBB),which were detected by immunohistochemistry.Inflammatory factors were examined by real-time polymerase chain reaction(RT-PCR)using tissue Ribonucleic Acid(RNA).The concentration of blood glucose is 15 mmol/L or more,which proved that the diabetes model was a success.Fasudil pretreatment decreases the percentage of stroke mortality of diabetes from 43.75%to 31.25%,while the infarction volume decreases from 52.95%±12.7%to 45.97%±6.7%.Gap formation of tight junction and Immunoglobulin G(Ig G)leakage were reduced(P<0.05),and the expression of inflammatory factors decreases(P<0.05)in fasudil pretreatment after diabetic stroke.Diabetes aggravates the mortality of cerebral ischemic rats.Prolonged fasudil pretreatment can reduce mortality of diabetic stroke,decrease cerebral infarction volume and undermine inflammatory factors expression,and protect the BBB.展开更多
基金the National Natural Science Foundation of China(Nos.50779033,81771244,81771251,81471178 and 81522015)the National Key Research and Development Program of China(No.2016YFC1300600)+1 种基金the K.C.Wong Education Foundationthe Fund of the Science and Technology Commission of Shanghai Municipality(No.17ZR1413600)
文摘Diabetes is usually associated with cerebrovascular disease,especially stroke.In practice,fasudil is widely accepted to be applied for the treatment of vascular disease.This article demonstrates the study concentrating on the effects of fasudil pretreatment on the prognosis of diabetic stroke.250—300 g SpragueDawley rats were randomly divided into three groups,non-diabetic stroke group,diabetic stroke group,and fasudil pretreatment group.The rats of diabetes group were treated with intraperitoneal injection of streptozotocin(60 mg/kg),in the meantime the same dose of citrate buffer was injected into those of the control group.The rats of the fasudil group received daily fasudil intraperitoneal injection at 10 mg/kg for three consecutive weeks.After four weeks,all the rats of the experimental group were treated with middle cerebral artery occlusion for 90 min.After sacrifice,the fresh brain samples were collected for following experiments,including infarct volume,edema volume,blood-brain barrier(BBB),which were detected by immunohistochemistry.Inflammatory factors were examined by real-time polymerase chain reaction(RT-PCR)using tissue Ribonucleic Acid(RNA).The concentration of blood glucose is 15 mmol/L or more,which proved that the diabetes model was a success.Fasudil pretreatment decreases the percentage of stroke mortality of diabetes from 43.75%to 31.25%,while the infarction volume decreases from 52.95%±12.7%to 45.97%±6.7%.Gap formation of tight junction and Immunoglobulin G(Ig G)leakage were reduced(P<0.05),and the expression of inflammatory factors decreases(P<0.05)in fasudil pretreatment after diabetic stroke.Diabetes aggravates the mortality of cerebral ischemic rats.Prolonged fasudil pretreatment can reduce mortality of diabetic stroke,decrease cerebral infarction volume and undermine inflammatory factors expression,and protect the BBB.
