Background Cotton is a strategically important fibre crop for global textile industry.It profoundly impacts several countries’industrial and agricultural sectors.Sustainable cotton production is continuously threaten...Background Cotton is a strategically important fibre crop for global textile industry.It profoundly impacts several countries’industrial and agricultural sectors.Sustainable cotton production is continuously threatened by the unpre-dictable changes in climate,specifically high temperatures.Breeding heat-tolerant,high-yielding cotton cultivars with wide adaptability to be grown in the regions with rising temperatures is one of the primary objectives of modern cotton breeding programmes.Therefore,the main objective of the current study is to figure out the effective breed-ing approach to imparting heat tolerance as well as the judicious utilization of commercially significant and stress-tolerant attributes in cotton breeding.Initially,the two most notable heat-susceptible(FH-115 and NIAB Kiran)and tolerant(IUB-13 and GH-Mubarak)cotton cultivars were spotted to develop filial and backcross populations to accom-plish the preceding study objectives.The heat tolerant cultivars were screened on the basis of various morphological(seed cotton yield per plant,ginning turnout percentage),physiological(pollen viability,cell membrane thermostabil-ity)and biochemical(peroxidase activity,proline content,hydrogen peroxide content)parameters.Results The results clearly exhibited that heat stress consequently had a detrimental impact on every studied plant trait,as revealed by the ability of crossing and their backcross populations to tolerate high temperatures.However,when considering overall yield,biochemical,and physiological traits,the IUB-13×FH-115 cross went over particularly well at both normal and high temperature conditions.Moreover,overall seed cotton yield per plant exhibited a posi-tive correlation with both pollen viability and antioxidant levels(POD activity and proline content).Conclusions Selection from segregation population and criteria involving pollen viability and antioxidant levels concluded to be an effective strategy for the screening of heat-tolerant cotton germplasms.Therefore,understanding acquired from this study can assist breeders identifying traits that should be prioritized in order to develop climate resilient cotton cultivars.展开更多
Cotton has enormous economic potential providing high-quality protein,oil,and fibre.A large increase in cotton output is necessary due to the world’s changing climate and constantly expanding human population.In the ...Cotton has enormous economic potential providing high-quality protein,oil,and fibre.A large increase in cotton output is necessary due to the world’s changing climate and constantly expanding human population.In the past,conventional breeding techniques were used to introduce genes into superior cotton cultivars to increase production and to improve quality.The disadvantages of traditional breeding techniques are their time-consuming,reliance on genetic differences that are already present,and considerable backcrossing.To accomplish goals in a short amount of time,contemporary plant breeding techniques,in particular modern genome editing technologies(GETs),can be used.Numerous crop improvement initiatives have made use of GETs,such as zinc-finger nucleases,transcription-activator-like effector nucleases,clustered regularly interspaced palindromic repeats(CRISPR),and CRISPR-associated proteins systems(CRISPR/Cas)-based technologies.The CRISPR/Cas system has a lot of potential because it combines three qualities that other GETs lack:simplicity,competence,and adaptability.The CRISPR/Cas mechanism can be used to improve cotton tolerance to biotic and abiotic stresses,alter gene expression,and stack genes for critical features with little possibility of segregation.The transgene clean strategy improves CRISPR acceptability addressing regulatory issues associated with the genetically modified organisms(GMOs).The research opportunities for using the CRISPR/Cas system to address biotic and abiotic stresses,fibre quality,plant architecture and blooming,epigenetic changes,and gene stacking for commercially significant traits are highlighted in this article.Furthermore,challenges to use of CRISPR technology in cotton and its potential for the future are covered in detail.展开更多
基金Centre for Advance Studies in Agricultural Food Security and Punjab Agricultural Research Board for providing funds under CAS-PARB project(No.964).
文摘Background Cotton is a strategically important fibre crop for global textile industry.It profoundly impacts several countries’industrial and agricultural sectors.Sustainable cotton production is continuously threatened by the unpre-dictable changes in climate,specifically high temperatures.Breeding heat-tolerant,high-yielding cotton cultivars with wide adaptability to be grown in the regions with rising temperatures is one of the primary objectives of modern cotton breeding programmes.Therefore,the main objective of the current study is to figure out the effective breed-ing approach to imparting heat tolerance as well as the judicious utilization of commercially significant and stress-tolerant attributes in cotton breeding.Initially,the two most notable heat-susceptible(FH-115 and NIAB Kiran)and tolerant(IUB-13 and GH-Mubarak)cotton cultivars were spotted to develop filial and backcross populations to accom-plish the preceding study objectives.The heat tolerant cultivars were screened on the basis of various morphological(seed cotton yield per plant,ginning turnout percentage),physiological(pollen viability,cell membrane thermostabil-ity)and biochemical(peroxidase activity,proline content,hydrogen peroxide content)parameters.Results The results clearly exhibited that heat stress consequently had a detrimental impact on every studied plant trait,as revealed by the ability of crossing and their backcross populations to tolerate high temperatures.However,when considering overall yield,biochemical,and physiological traits,the IUB-13×FH-115 cross went over particularly well at both normal and high temperature conditions.Moreover,overall seed cotton yield per plant exhibited a posi-tive correlation with both pollen viability and antioxidant levels(POD activity and proline content).Conclusions Selection from segregation population and criteria involving pollen viability and antioxidant levels concluded to be an effective strategy for the screening of heat-tolerant cotton germplasms.Therefore,understanding acquired from this study can assist breeders identifying traits that should be prioritized in order to develop climate resilient cotton cultivars.
文摘Cotton has enormous economic potential providing high-quality protein,oil,and fibre.A large increase in cotton output is necessary due to the world’s changing climate and constantly expanding human population.In the past,conventional breeding techniques were used to introduce genes into superior cotton cultivars to increase production and to improve quality.The disadvantages of traditional breeding techniques are their time-consuming,reliance on genetic differences that are already present,and considerable backcrossing.To accomplish goals in a short amount of time,contemporary plant breeding techniques,in particular modern genome editing technologies(GETs),can be used.Numerous crop improvement initiatives have made use of GETs,such as zinc-finger nucleases,transcription-activator-like effector nucleases,clustered regularly interspaced palindromic repeats(CRISPR),and CRISPR-associated proteins systems(CRISPR/Cas)-based technologies.The CRISPR/Cas system has a lot of potential because it combines three qualities that other GETs lack:simplicity,competence,and adaptability.The CRISPR/Cas mechanism can be used to improve cotton tolerance to biotic and abiotic stresses,alter gene expression,and stack genes for critical features with little possibility of segregation.The transgene clean strategy improves CRISPR acceptability addressing regulatory issues associated with the genetically modified organisms(GMOs).The research opportunities for using the CRISPR/Cas system to address biotic and abiotic stresses,fibre quality,plant architecture and blooming,epigenetic changes,and gene stacking for commercially significant traits are highlighted in this article.Furthermore,challenges to use of CRISPR technology in cotton and its potential for the future are covered in detail.
