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籽瓜、黏籽和普通西瓜的果实代谢组比较 被引量:8
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作者 袁平丽 何楠 +6 位作者 赵胜杰 路绪强 朱红菊 刁卫楠 龚成胜 muhammad jawad umer 刘文革 《中国农业科学》 CAS CSCD 北大核心 2021年第19期4179-4195,共17页
【目的】西瓜是一种广受欢迎的夏季水果,籽用西瓜(籽瓜)、黏籽西瓜和普通西瓜是3种重要的栽培类型,但对其果实中代谢物全面鉴定的研究很少,比较这3种西瓜果实的代谢组学差异,为明确驯化改良对不同类型西瓜代谢组的影响提供新见解。【方... 【目的】西瓜是一种广受欢迎的夏季水果,籽用西瓜(籽瓜)、黏籽西瓜和普通西瓜是3种重要的栽培类型,但对其果实中代谢物全面鉴定的研究很少,比较这3种西瓜果实的代谢组学差异,为明确驯化改良对不同类型西瓜代谢组的影响提供新见解。【方法】试验以5份籽用西瓜、5份黏籽西瓜和6份普通西瓜为材料,对成熟期果肉进行广泛靶向代谢组学检测,采用SIMCA-P、MetaboAnalyst 5.0、Origin等软件对代谢组数据进行分析。【结果】LC-MS分析共检测到323种代谢物,包括51种氨基酸及其衍生物、21种核苷酸及其衍生物、14种碳水化合物、32种有机酸、52种脂质、36种类黄酮、32种羟基肉桂酰衍生物等。PCA和聚类分析显示,籽用西瓜和黏籽西瓜的代谢组学轮廓差异较小,籽用西瓜的代谢组轮廓介于黏籽西瓜和普通西瓜之间。对分类贡献率较大的代谢物是蔗糖、柠檬酸、L-谷氨酸、L-苏氨酸、葫芦素类、香草酸糖苷异构体、脂质类等。聚类热图分析显示黏籽西瓜中特有的代谢物是葫芦素及其衍生物等,籽用西瓜中的绿原酸、LysoPE脂质类等化合物含量较高,普通西瓜中的糖类、精氨酸、阿魏酸及C18-2和C18-3不饱和脂肪酸等物质含量较高。比较分析共鉴定出156种差异代谢物,籽用西瓜与黏籽西瓜的主要差异代谢物有对香豆醛、阿魏酸、肉桂酸、蔗糖、葫芦素D O-葡萄糖苷、葫芦素E异构体、牡荆素、松柏醇等。籽用西瓜与普通西瓜的主要差异代谢物有脂质类、类黄酮类、有机酸类和糖类等。随着作物进化的程度增加,营养类代谢物质含量增加,抗性相关的代谢物质含量减少。【结论】籽用西瓜和黏籽西瓜的果实代谢组差异比籽用西瓜和普通西瓜的差异小,籽用西瓜的代谢轮廓介于黏籽西瓜和普通西瓜之间。除了表型和基因组差异外,代谢组差异也可以作为区分品种的重要依据。本研究首次在代谢组学水平比较了3种类型西瓜果实的差异,对深入理解西瓜种质资源,培育保健型西瓜新品种有重要的指导意义。 展开更多
关键词 黏籽西瓜 籽用西瓜 普通西瓜 代谢组学 差异代谢物 驯化改良
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黏籽西瓜、籽瓜和普通西瓜的植物学性状比较分析 被引量:3
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作者 袁平丽 龚成胜 +9 位作者 何楠 刁卫楠 muhammad jawad umer 朱红菊 杨东东 muhammad Anees 路绪强 Kaseb M O 赵胜杰 刘文革 《中国瓜菜》 CAS 北大核心 2021年第9期15-25,共11页
西瓜属于葫芦科的重要水果作物,栽培类型丰富多样,其中黏籽西瓜、籽瓜和普通西瓜是被人类栽培和食用的主要类型,但是这3种类型西瓜的植物学性状差异并没有被系统调查过。针对18份黏籽西瓜、38份籽瓜、174份普通西瓜品种的14个植物学性... 西瓜属于葫芦科的重要水果作物,栽培类型丰富多样,其中黏籽西瓜、籽瓜和普通西瓜是被人类栽培和食用的主要类型,但是这3种类型西瓜的植物学性状差异并没有被系统调查过。针对18份黏籽西瓜、38份籽瓜、174份普通西瓜品种的14个植物学性状进行调查和统计分析,结果显示,供试西瓜品种的植物学性状变异范围大,不同性状在3种类型西瓜之间的差异显著,籽瓜和黏籽西瓜的表型更相似。系统聚类和PCA分析可以将3种类型的西瓜品种基本区分开来,籽瓜介于黏籽西瓜和普通西瓜之间。该研究结果有助于充分了解和利用西瓜种质资源,能够为培育理想株型和优质的西瓜新品种提供科学依据。 展开更多
关键词 黏籽西瓜 籽瓜 普通西瓜 植物学性状
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Identification of an Aux/IAA regulator for flesh firmness using combined GWAS and bulked segregant RNA-Seq analysis in watermelon 被引量:1
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作者 muhammad Anees Hongju Zhu +8 位作者 muhammad jawad umer Chengsheng Gong Pingli Yuan Xuqiang Lu Nan He Mohamed Omar Kaseb Dongdong Yang Yong Zhao Wenge Liu 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第5期1198-1213,共16页
Watermelon is a highly cultivated fruit crop renowned for its quality properties of fruit flesh.Among various quality factors,fruit flesh firmness is a crucial quality parameter influencing the fruit texture,shelf lif... Watermelon is a highly cultivated fruit crop renowned for its quality properties of fruit flesh.Among various quality factors,fruit flesh firmness is a crucial quality parameter influencing the fruit texture,shelf life and its commercial value.The auxin/indole-3-acetic acid(Aux/IAA)plays a significant role in fruit development and ripening of non-climacteric fruits.However,the regulatory mechanism of Aux/IAA in controlling fruit flesh firmness and ripening in watermelon remains unknown.In this study,we employed an integrative approach combining genome-wide association study(GWAS)and bulked segregant RNA-Seq analysis(BSR-Seq)to identify an overlapping candidate region between 12776310 and 12968331 bp on chromosome 6,underlying an auxin-responsive gene(Aux/IAA)associated with flesh firmness in watermelon.Transcriptome analysis,followed by real-time quantitative reverse transcription PCR(qRT-PCR),confirmed that the expression of Aux/IAA was consistently higher in fruits with high flesh firmness.The sequence alignment revealed a single base mutation in the coding region of Aux/IAA.Furthermore,the concomitant Kompetitive/Competitive allele-specific PCR(KASP)genotyping data sets for F2 population and germplasm accessions identified Aux/IAA as a strong candidate gene associated with flesh firmness.Aux/IAA was enriched in the plant hormone signal transduction pathway,involving cell enlargement and leading to low flesh firmness.We determined the higher accumulation of abscisic acid(ABA)in fruits with low flesh firmness than hard flesh.Moreover,overexpression of Aux/IAA induced higher flesh firmness with an increased number of fruit flesh cells while reducing ABA content and flesh cell sizes.Additionally,the allelic variation in Aux/IAA for soft flesh firmness was found to exist in Citrullus mucosospermus and gradually fixed into Citrullus lanatus during domestication,indicating that soft flesh firmness was a domesticated trait.These findings significantly enhanced our understanding of watermelon fruit flesh firmness and consequently the watermelon fruit quality. 展开更多
关键词 WATERMELON Flesh firmness GWAS BSR-Seq KASP marker analysis Transient overexpression
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Identification of key gene networks controlling organic acid and sugar metabolism during watermelon fruit development by integrating metabolic phenotypes and gene expression profiles 被引量:16
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作者 muhammad jawad umer Luqman Bin Safdar +9 位作者 Haileslassie Gebremeskel Shengjie Zhao Pingli Yuan Hongju Zhu M.O.Kaseb muhammad Anees Xuqiang Lu Nan He Chengsheng Gong Wenge Liu 《Horticulture Research》 SCIE 2020年第1期191-203,共13页
The organoleptic qualities of watermelon fruit are defined by the sugar and organic acid contents,which undergo considerable variations during development and maturation.The molecular mechanisms underlying these varia... The organoleptic qualities of watermelon fruit are defined by the sugar and organic acid contents,which undergo considerable variations during development and maturation.The molecular mechanisms underlying these variations remain unclear.In this study,we used transcriptome profiles to investigate the coexpression patterns of gene networks associated with sugar and organic acid metabolism.We identified 3 gene networks/modules containing 2443 genes highly correlated with sugars and organic acids.Within these modules,based on intramodular significance and Reverse Transcription Quantitative polymerase chain reaction(RT-qPCR),we identified 7 genes involved in the metabolism of sugars and organic acids.Among these genes,Cla97C01G000640,Cla97C05G087120 and Cla97C01G018840(r^(2)=0.83 with glucose content)were identified as sugar transporters(SWEET,EDR6 and STP)and Cla97C03G064990(r^(2)=0.92 with sucrose content)was identified as a sucrose synthase from information available for other crops.Similarly,Cla97C07G128420,Cla97C03G068240 and Cla97C01G008870,having strong correlations with malic(r^(2)=0.75)and citric acid(r^(2)=0.85),were annotated as malate and citrate transporters(ALMT7,CS,and ICDH).The expression profiles of these 7 genes in diverse watermelon genotypes revealed consistent patterns of expression variation in various types of watermelon.These findings add significantly to our existing knowledge of sugar and organic acid metabolism in watermelon. 展开更多
关键词 METABOLISM SUGAR NETWORKS
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HD-Zip Transcription Factor is Responsible for No-Lobed Leaf in Watermelon(Citrullus lanatus L.) 被引量:1
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作者 Shixiang Duan Yaomiao Guo +8 位作者 Yinping Wang muhammad jawad umer Dongming Liu Sen Yang Huanhuan Niu Shouru Sun Luming Yang Junling Dou Huayu Zhu 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第5期1311-1328,共18页
Leaf is a vital organ of plants that plays an essential role in photosynthesis and respiration.As an important agronomic trait in leaf development,leaf shape is classified into lobed,entire(no-lobed),and serrated in m... Leaf is a vital organ of plants that plays an essential role in photosynthesis and respiration.As an important agronomic trait in leaf development,leaf shape is classified into lobed,entire(no-lobed),and serrated in most crops.In this study,two-lobed leaf watermelon inbred lines WT2 and WCZ,and a no-lobed leaf watermelon inbred line WT20 were used to create two F_(2)populations.Segregation analysis suggested that lobed leaves were dominant over the no-lobed leaves,and it was controlled by a signal gene.A locus on watermelon chromosome 4 controlling watermelon lobed/no-lobed leaves was identified through BSA-seq strategy combined with linkage analysis.The candidate gene was fine-mapped to a 61.5 kb region between 21,224,481 and 21,285,957 bp on watermelon chromosome 4 using two F_(2)populations.Four functional genes were annotated in the candidate region,while sequences blast showed that there was a single-base deletion(A/-)only in the exon of Cla018360,which resulted in premature termination of translation in the no-lobed leaf lines.Function prediction showed that Cla018360 encodes an HD-Zip protein that has been reported to regulate the development of leaf shape.The single-base deletion also occurred in the HD-Zip domain.We inferred that the Cla018360 gene is the candidate gene for regulating the development of lobed/no-lobed leaves in watermelon.Gene expression analysis showed that Cla018360 was highly expressed in young leaves.Phylogenetic analysis showed that Cla018360 had a close genetic relationship with AtHB51,which had been reported to regulate the formation of leaf shape in Arabidopsis.Furthermore,transcriptome analysis showed that a total of 333 differentially expressed genes were identified between WT2 and WT20,of which 115 and 218 genes were upregulated and downregulated in no-lobed leaved watermelon WT20.This study not only provides a good entry point for studying leaf development but also provides foundational insights into breeding for special plant architecture in watermelon. 展开更多
关键词 WATERMELON lobed/no-lobed leaf mapping RNA-SEQ
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Widespread incomplete lineage sorting and introgression shaped adaptive radiation in the Gossypium genus
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作者 Yanchao Xu Yangyang Wei +14 位作者 Zhongli Zhou Xiaoyan Cai Scott ABoden muhammad jawad umer Luqman BSafdar Yuling Liu Dingsha Jin Yuqing Hou Yuhong Wang Sarah Brooke Wall Kunbo Wang Shuxun Yu Baohong Zhang Renhai Peng Fang Liu 《Plant Communications》 SCIE CSCD 2024年第2期185-197,共13页
Cotton(Gossypium)stands as a crucial economic crop,serving as the primary source of naturalfiber for the textile sector.However,the evolutionary mechanisms driving speciation within the Gossypium genus remain unresolv... Cotton(Gossypium)stands as a crucial economic crop,serving as the primary source of naturalfiber for the textile sector.However,the evolutionary mechanisms driving speciation within the Gossypium genus remain unresolved.In this investigation,we leveraged 25 Gossypium genomes and introduced four novel assem-blies—G.harknessii,G.gossypioides,G.trilobum,and G.klotzschianum(Gklo)—to delve into the speciation history of this genus.Notably,we encountered intricate phylogenies potentially stemming from introgres-sion.These complexities are further compounded by incomplete lineage sorting(ILS),a factor likely to have been instrumental in shaping the swift diversification of cotton.Our focus subsequently shifted to the rapid radiation episode during a concise period in Gossypium evolution.For a recently diverged lineage comprising G.davidsonii,Gklo,and G.raimondii,we constructed afinely detailed ILS map.Intriguingly,this analysis revealed the non-random distribution of ILS regions across the reference Gklo genome.Moreover,we identified signs of robust natural selection influencing specific ILS regions.Noteworthy variations per-taining to speciation emerged between the closely related sister species Gklo and G.davidsonii.Approxi-mately 15.74%of speciation structural variation genes and 12.04%of speciation-associated genes were esti-mated to intersect with ILS signatures.Thesefindings enrich our understanding of the role of ILS in adaptive radiation,shedding fresh light on the intricate speciation history of the Gossypium genus. 展开更多
关键词 cotton speciation Gossypium genus incomplete lineage sorting ILS phylogenetic analysis gene tree resolution
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