目的:利用静息态功能磁共振成像(rs-fMRI)技术的低频振幅(ALFF)算法探讨针刺治疗儿童屈光参差性弱视的脑功能机制。方法:将80例屈光层参差性单眼弱视儿童分为常规组(40例,脱落1例)和针刺组(40例,脱落1例)。常规组予以配镜、红闪光栅和...目的:利用静息态功能磁共振成像(rs-fMRI)技术的低频振幅(ALFF)算法探讨针刺治疗儿童屈光参差性弱视的脑功能机制。方法:将80例屈光层参差性单眼弱视儿童分为常规组(40例,脱落1例)和针刺组(40例,脱落1例)。常规组予以配镜、红闪光栅和视刺激治疗,每项5 min/次;针刺组在常规治疗的基础上给予“调气通经明目”针法治疗,取双侧睛明、攒竹、光明、风池,每次留针30 m in;两组均隔日治疗1次,3次/周,共4周。治疗结束后,检测两组患者视力和视觉诱发电位(P-VEP)P100波潜伏期和振幅改变。同时从两组中各随机选取9例左眼弱视患儿,于治疗前后分别予以rs-fMRI扫描,比较分析两组患儿脑区ALFF差异。结果:①治疗后两组患儿矫正视力均较治疗前提高(P<0.05);与治疗前比较,治疗后两组患儿P-VEP P100波潜伏期缩短、振幅升高(P<0.05),针刺组改善程度显著优于常规组(P<0.05)。②治疗后,常规组右侧豆状核ALFF值增高;针刺组右侧颞叶ALFF值增高(P<0.05);针刺组与常规组比较,右侧边缘叶/扣带回左侧胼胝体/扣带回、左侧额叶/双侧辅助运动区的ALFF值降低(P<0.05),右侧顶叶/顶下小叶增高(P<0.05)。结论:针刺联合常规治疗可改善屈光参差性弱视儿童的矫正视力,增强视神经传导功能;两种疗法的效应机制与有效调节不同脑区自发功能活动和响应有关,但二者脑功能响应区域存在明显差异。与常规疗法相比,边缘系统、额叶、顶叶可能是针刺治疗屈光参差性弱视重点调控的脑区。展开更多
Objective: To explore the mechanism underlying acupuncture regulation of the visual cortex plasticity. Methods: Eighteen kittens of four weeks were randomly divided into 3 groups, a normal group, a model group and an ...Objective: To explore the mechanism underlying acupuncture regulation of the visual cortex plasticity. Methods: Eighteen kittens of four weeks were randomly divided into 3 groups, a normal group, a model group and an acupuncture group, with six in each group. There was no treatment to those in the normal group. Unilateral eyelid suture method was used to establish the deprivation amblyopia cat model in the model group and the acupuncture group. After that, kittens in the model group didn't receive any treatment, but those in the acupuncture group were treated with acupuncture therapy of 12 weeks. Pattern Visual Evoked Potential (P-VEP) and c-Fos protein expression of visual cortex of kittens in each group were tested before and after acupuncture treatment. Results: P-VEP waveform changed significantly in kittens of the model group, the time value of P 100 was significantly delayed (P<0.01) and N 45 -P 100 amplitude was significantly lower (P<0.01) compared with the normal group. After treatment, the time value of P 100 in kittens of the acupuncture group was significantly shorter (P<0.01) and N 45 -P 100 amplitude was significantly higher (P<0.01) when compared with the model group. Expression of c-Fos positive neurons can be seen in the visual cortex layers II-IV of kittens in the acupuncture group, and the density and percentage of c-Fos immunoreactive neurons of cortex layers II-IV in kittens of the model group were significantly lower than those in the acupuncture group. Conclusion: Acupuncture has obvious improvement for abnormal changes of P-VEP waveform of monocular visual deprivation kittens; it can also increase the c-Fos protein expression in visual cortex after form- deprived.展开更多
文摘目的:利用静息态功能磁共振成像(rs-fMRI)技术的低频振幅(ALFF)算法探讨针刺治疗儿童屈光参差性弱视的脑功能机制。方法:将80例屈光层参差性单眼弱视儿童分为常规组(40例,脱落1例)和针刺组(40例,脱落1例)。常规组予以配镜、红闪光栅和视刺激治疗,每项5 min/次;针刺组在常规治疗的基础上给予“调气通经明目”针法治疗,取双侧睛明、攒竹、光明、风池,每次留针30 m in;两组均隔日治疗1次,3次/周,共4周。治疗结束后,检测两组患者视力和视觉诱发电位(P-VEP)P100波潜伏期和振幅改变。同时从两组中各随机选取9例左眼弱视患儿,于治疗前后分别予以rs-fMRI扫描,比较分析两组患儿脑区ALFF差异。结果:①治疗后两组患儿矫正视力均较治疗前提高(P<0.05);与治疗前比较,治疗后两组患儿P-VEP P100波潜伏期缩短、振幅升高(P<0.05),针刺组改善程度显著优于常规组(P<0.05)。②治疗后,常规组右侧豆状核ALFF值增高;针刺组右侧颞叶ALFF值增高(P<0.05);针刺组与常规组比较,右侧边缘叶/扣带回左侧胼胝体/扣带回、左侧额叶/双侧辅助运动区的ALFF值降低(P<0.05),右侧顶叶/顶下小叶增高(P<0.05)。结论:针刺联合常规治疗可改善屈光参差性弱视儿童的矫正视力,增强视神经传导功能;两种疗法的效应机制与有效调节不同脑区自发功能活动和响应有关,但二者脑功能响应区域存在明显差异。与常规疗法相比,边缘系统、额叶、顶叶可能是针刺治疗屈光参差性弱视重点调控的脑区。
基金supported by National Natural Science Foundation of China (81260560)
文摘Objective: To explore the mechanism underlying acupuncture regulation of the visual cortex plasticity. Methods: Eighteen kittens of four weeks were randomly divided into 3 groups, a normal group, a model group and an acupuncture group, with six in each group. There was no treatment to those in the normal group. Unilateral eyelid suture method was used to establish the deprivation amblyopia cat model in the model group and the acupuncture group. After that, kittens in the model group didn't receive any treatment, but those in the acupuncture group were treated with acupuncture therapy of 12 weeks. Pattern Visual Evoked Potential (P-VEP) and c-Fos protein expression of visual cortex of kittens in each group were tested before and after acupuncture treatment. Results: P-VEP waveform changed significantly in kittens of the model group, the time value of P 100 was significantly delayed (P<0.01) and N 45 -P 100 amplitude was significantly lower (P<0.01) compared with the normal group. After treatment, the time value of P 100 in kittens of the acupuncture group was significantly shorter (P<0.01) and N 45 -P 100 amplitude was significantly higher (P<0.01) when compared with the model group. Expression of c-Fos positive neurons can be seen in the visual cortex layers II-IV of kittens in the acupuncture group, and the density and percentage of c-Fos immunoreactive neurons of cortex layers II-IV in kittens of the model group were significantly lower than those in the acupuncture group. Conclusion: Acupuncture has obvious improvement for abnormal changes of P-VEP waveform of monocular visual deprivation kittens; it can also increase the c-Fos protein expression in visual cortex after form- deprived.
