目的观察泛素连接酶肌肉环指状蛋白2(muscle ring finger protein2,MuRF2)在HL-1心肌细胞自噬中的作用。方法采用慢病毒转染技术上调和下调HL-1心肌细胞中MuRF2的表达。实验分为阴性对照组、阳性对照组(空载病毒对照)、雷帕霉素处理组、...目的观察泛素连接酶肌肉环指状蛋白2(muscle ring finger protein2,MuRF2)在HL-1心肌细胞自噬中的作用。方法采用慢病毒转染技术上调和下调HL-1心肌细胞中MuRF2的表达。实验分为阴性对照组、阳性对照组(空载病毒对照)、雷帕霉素处理组、MuRF2过表达组和MuRF2敲减组。用雷帕霉素处理细胞,蛋白免疫印迹法(Western blot)检测自噬蛋白LC3和BNIP3的表达。透射电镜观察HL-1心肌细胞超微结构。结果(1)Western blot验证,MuRF2过表达组中MuRF2蛋白表达水平均高于阴性对照组、阳性对照组、雷帕霉素处理组(P均<0.05),MuRF2敲减组中MuRF2蛋白表达水平均低于阴性对照组、阳性对照组、雷帕霉素处理组(P均<0.05)。(2)Western blot结果表明,雷帕霉素处理组LC3蛋白Ⅱ/Ⅰ比值和BNIP3蛋白表达均高于阴性对照组(P均<0.05),MuRF2过表达组LC3蛋白Ⅱ/Ⅰ比值和BNIP3蛋白表达均低于雷帕霉素处理组(P均<0.05)。与阴性对照组相比,MuRF2敲减组的LC3蛋白Ⅱ/Ⅰ比值和BNIP3蛋白表达均升高(P均<0.05),雷帕霉素处理组和MuRF2敲减组在LC蛋白Ⅱ/Ⅰ比值和BNIP3蛋白表达上差异均无统计学意义(P均>0.05)。透射电镜显示,雷帕霉素处理组线粒体分裂、碎片化,自噬体和自噬溶酶体散在胞质内。MuRF2过表达组线粒体处于融合状态,自噬体明显减少。结论MuRF2可以抑制心肌细胞线粒体自噬。展开更多
Firstly,the daily variations of NO2 concentration in the urban area of Wanzhou in 2012 were analyzed,and then the relationship between NO2 concentration and meteorological factors（ precipitation,atmospheric pressure,...Firstly,the daily variations of NO2 concentration in the urban area of Wanzhou in 2012 were analyzed,and then the relationship between NO2 concentration and meteorological factors（ precipitation,atmospheric pressure,wind speed,temperature,relative humidity and sunshine hours） was discussed. Finally,the multiple linear regression equation was established to predict NO2 concentration. The results showed that NO2 concentration in the urban area of Wanzhou did not exceed 80 μg /m3in most days from January 1 to December 31 in 2012. Among the six meteorological factors,NO2 concentration correlated significantly with three meteorological factors,that is,NO2 concentration correlated negatively with atmospheric pressure and wind speed but positively with relative humidity. NO2 concentration in the urban area of Wanzhou could be predicted using the multiple linear regression model. According to the rose diagram of wind directions,the wind blowing from the NNW was dominant in the urban area of Wanzhou.展开更多
基金Supported by the Municipal Key Laboratory Project of Colleges and Universities in Chongqing City,China(WEPKL2013MS-10)National Innovation Planning Project for University Students in 2013(201310643003)
文摘Firstly,the daily variations of NO2 concentration in the urban area of Wanzhou in 2012 were analyzed,and then the relationship between NO2 concentration and meteorological factors（ precipitation,atmospheric pressure,wind speed,temperature,relative humidity and sunshine hours） was discussed. Finally,the multiple linear regression equation was established to predict NO2 concentration. The results showed that NO2 concentration in the urban area of Wanzhou did not exceed 80 μg /m3in most days from January 1 to December 31 in 2012. Among the six meteorological factors,NO2 concentration correlated significantly with three meteorological factors,that is,NO2 concentration correlated negatively with atmospheric pressure and wind speed but positively with relative humidity. NO2 concentration in the urban area of Wanzhou could be predicted using the multiple linear regression model. According to the rose diagram of wind directions,the wind blowing from the NNW was dominant in the urban area of Wanzhou.
