BACKGROUND： There are no reports about adult degenerative disc cell culture model currently. This establishment of disc cell culture is the cellular basis of intervertebral disc tissue engineering. OBJECTIVE： To est...BACKGROUND： There are no reports about adult degenerative disc cell culture model currently. This establishment of disc cell culture is the cellular basis of intervertebral disc tissue engineering. OBJECTIVE： To establish cell culture models from human degenerative intervertebral disc, and to settle the cytology base of disc tissue engineering. DESIGN AND SETTING： The controlled observational experiment with regard to cytology was performed in Shandong Institute of Orthopaedics and Traumatology. MATERIALS： Five degenerative intervertebral disc patients were recruited from Department of Orthopaedics, Affiliated Hospital of Qingdao University Medical College, including one case in L2-3, three cases in L4-5 and one case in L5-S1. METHODS： Five samples were taken from the operational disectomy for lumbar disc herniation, and these samples were cultured using enzymatic digestion cell culture and tissue cell culture method differently. All the samples were cultured with HAMF12 medium with the addition of 10% and 20% fetal bovine serum. The morphology of the cultured cell was observed with Giemsa staining and transmission electron microscopy respectively. MAIN OUTCOME MEASURES： ①Cellular growth; ②Morphology of the cultured cells; ③Ultrastructure of the cultured cells. RESULTS： By means of enzymatic digestion cell culture and tissue cell culture method, numerous degenerative lumbar disc cells were obtained and successfully subcultured. The cell secretions around the cultured cells may influence the growth of these cells. Destroying the secretions, the cultured cells could be highly proliferated. The degenerative intervertebral disc cells were proliferated vigorously in HAMF12 medium with 20% fetal bovine serum compared with that with 10% fetal bovine serum. The notochordal cell was observed in all the specimens. CONCLUSION： The adult degenerative disc can be used to obtain the required cell for tissue engineering.展开更多
由于传统无迹卡尔曼滤波估算方法具有局限性,为了能准确估算动力电池荷电状态(state of charge,SOC),提出了一种基于无迹卡尔曼粒子滤波的动力电池SOC估算方法.以三元锂电池为研究对象,建立了电池二阶RC等效电路模型,通过对电池进行充...由于传统无迹卡尔曼滤波估算方法具有局限性,为了能准确估算动力电池荷电状态(state of charge,SOC),提出了一种基于无迹卡尔曼粒子滤波的动力电池SOC估算方法.以三元锂电池为研究对象,建立了电池二阶RC等效电路模型,通过对电池进行充放电试验辨识出模型参数,并验证模型准确性.采集了实际工况下的电池数据,分别用无迹卡尔曼滤波算法、粒子滤波算法和无迹卡尔曼粒子滤波算法估算电池SOC,在MATLAB中进行了仿真试验,并对估算的电池SOC进行比较.结果表明:无迹卡尔曼粒子滤波算法可以快速准确地估算出电池SOC,误差小于2.5%,优于另外2种算法.展开更多
文摘BACKGROUND： There are no reports about adult degenerative disc cell culture model currently. This establishment of disc cell culture is the cellular basis of intervertebral disc tissue engineering. OBJECTIVE： To establish cell culture models from human degenerative intervertebral disc, and to settle the cytology base of disc tissue engineering. DESIGN AND SETTING： The controlled observational experiment with regard to cytology was performed in Shandong Institute of Orthopaedics and Traumatology. MATERIALS： Five degenerative intervertebral disc patients were recruited from Department of Orthopaedics, Affiliated Hospital of Qingdao University Medical College, including one case in L2-3, three cases in L4-5 and one case in L5-S1. METHODS： Five samples were taken from the operational disectomy for lumbar disc herniation, and these samples were cultured using enzymatic digestion cell culture and tissue cell culture method differently. All the samples were cultured with HAMF12 medium with the addition of 10% and 20% fetal bovine serum. The morphology of the cultured cell was observed with Giemsa staining and transmission electron microscopy respectively. MAIN OUTCOME MEASURES： ①Cellular growth; ②Morphology of the cultured cells; ③Ultrastructure of the cultured cells. RESULTS： By means of enzymatic digestion cell culture and tissue cell culture method, numerous degenerative lumbar disc cells were obtained and successfully subcultured. The cell secretions around the cultured cells may influence the growth of these cells. Destroying the secretions, the cultured cells could be highly proliferated. The degenerative intervertebral disc cells were proliferated vigorously in HAMF12 medium with 20% fetal bovine serum compared with that with 10% fetal bovine serum. The notochordal cell was observed in all the specimens. CONCLUSION： The adult degenerative disc can be used to obtain the required cell for tissue engineering.
文摘由于传统无迹卡尔曼滤波估算方法具有局限性,为了能准确估算动力电池荷电状态(state of charge,SOC),提出了一种基于无迹卡尔曼粒子滤波的动力电池SOC估算方法.以三元锂电池为研究对象,建立了电池二阶RC等效电路模型,通过对电池进行充放电试验辨识出模型参数,并验证模型准确性.采集了实际工况下的电池数据,分别用无迹卡尔曼滤波算法、粒子滤波算法和无迹卡尔曼粒子滤波算法估算电池SOC,在MATLAB中进行了仿真试验,并对估算的电池SOC进行比较.结果表明:无迹卡尔曼粒子滤波算法可以快速准确地估算出电池SOC,误差小于2.5%,优于另外2种算法.
