An introgression line RBPH660,derived from wild rice Oryza rufipogon,showed stable resistance to brown planthopper(BPH).Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs.By...An introgression line RBPH660,derived from wild rice Oryza rufipogon,showed stable resistance to brown planthopper(BPH).Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs.By using the bulked segregant analysis(BSA)-seq method,two genomic regions harboring QTLs resistance to BPH were identified from 1.20 to 16.70 Mb on chromosome 4 and from 10.20 to 12.60 Mb on chromosome 9 in RBPH660,respectively.A major resistance locus,designated as Bph35 accounting for 51.27%of the phenotypic variation with a LOD score of 42.51,was mapped to the candidate region of chromosome 4 between In Del(insertion-deletion)markers PSM16 and R4 M13.For fine mapping of Bph35,one simple sequence repeat and three newly developed In Del markers were used to screen the recombinants.Finally,the Bph35 locus was delimited in the region from 6.28 to 6.93 Mb and there were 18 predicted protein-encoding genes with a total of 114 non-synonymous single nucleotide polymorphism(SNP)variant sites between the resistant and susceptible parents.Out of these genes,Os04 g0193950,encoding a putative NB-ARC(nucleotidebinding adaptor shared by APAF-1,R proteins and CED-4)and LRR(leucine-rich repeat)domain protein with nine non-synonymous SNP substitutions in its coding sequence regions,might be the candidate gene for Bph35.These findings would facilitate the map-based cloning of the Bph35 gene and development of resistant varieties against BPH in rice.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.31860416 and 31460387)the Natural Science Foundation of Guangxi Province of China(Grant Nos.2015GXNS FAA139083,2016GXNSFAA380032 and 2017GXNS FAA198314)the Key Project of Science and Technology of Guangxi Province of China(Grant Nos.Guike AA17204070,AB16380079 and AB16380093)。
文摘An introgression line RBPH660,derived from wild rice Oryza rufipogon,showed stable resistance to brown planthopper(BPH).Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs.By using the bulked segregant analysis(BSA)-seq method,two genomic regions harboring QTLs resistance to BPH were identified from 1.20 to 16.70 Mb on chromosome 4 and from 10.20 to 12.60 Mb on chromosome 9 in RBPH660,respectively.A major resistance locus,designated as Bph35 accounting for 51.27%of the phenotypic variation with a LOD score of 42.51,was mapped to the candidate region of chromosome 4 between In Del(insertion-deletion)markers PSM16 and R4 M13.For fine mapping of Bph35,one simple sequence repeat and three newly developed In Del markers were used to screen the recombinants.Finally,the Bph35 locus was delimited in the region from 6.28 to 6.93 Mb and there were 18 predicted protein-encoding genes with a total of 114 non-synonymous single nucleotide polymorphism(SNP)variant sites between the resistant and susceptible parents.Out of these genes,Os04 g0193950,encoding a putative NB-ARC(nucleotidebinding adaptor shared by APAF-1,R proteins and CED-4)and LRR(leucine-rich repeat)domain protein with nine non-synonymous SNP substitutions in its coding sequence regions,might be the candidate gene for Bph35.These findings would facilitate the map-based cloning of the Bph35 gene and development of resistant varieties against BPH in rice.
文摘“荥泽”古湖曾被大量史书文献记载,现已消亡,郑州周边地区地表以下0.60~27.00 m范围内存在不同厚度的湖沼相地层,岩性主要为灰黑色淤泥、淤泥质细砂,灰黑色淤泥质黏土、灰褐色黏土、灰褐色粉砂质黏土、灰黑色黏土质粉砂等等。通过岩矿鉴定、孢粉特征分析、同位素年代学、粒度分析、磁化率分析等手段对沉积物进行了系统研究,初步得出该古湖泊的形成可能始于晚更新世晚期,8000~3000 a BP曾大范围存在,沉积中心位于贾河村附近,古湖泊水源来自于黄河洪水和地表水径流的补给。沉积记录了“荥泽”古湖形成前期—形成期—鼎盛期—动荡衰退期—消亡期的全过程,结合地貌和史书古籍记载、各个时期的考古遗迹点位置、湖沼相层测年数据,初步界定鼎盛时期湖泊的西边界和南边界,分别位于荥阳市汜水镇和管城回族区十八里河镇、南曹乡附近。初步估算鼎盛时期湖泊面积超过1000 km^(2)。
