The methanolic extract of red seaweed Kappaphycus alvarezii was evaluated against three different cancer cell lines to study for its antiproliferative effect. Lung cancer cell line （NCIH 460）, Colon cancer cell line...The methanolic extract of red seaweed Kappaphycus alvarezii was evaluated against three different cancer cell lines to study for its antiproliferative effect. Lung cancer cell line （NCIH 460）, Colon cancer cell line （HCT 116） and Glial cell carcinoma （U 251） are the three selected cell lines investigated in this study. Different concentrations of methanol extract （0.01, 0.10, 1.00, 10.00 and 100.00 lag/mL respectively） of Kappaphycus alvarezii were prepared and screened by quantitative MTT （Microculture Tetrazolium） （3-（4, 5-dimethylthiazol）-2, 5-diphenyltetrazolium bromide） assay. MTT assay are the colorimetric assay which was applied to assess the viability and proliferation of cancer cells to determine the cytotoxicity of methanol extract ofK. alvarezii. The MTT test is based on the enzymatic reduction of the tetrazolium salt （MTT） to formazon crystals exclusively in living metabolically active cells developed purple color complex which was directly proportional to the viability of cells. To elucidate the in-vitro anticancer activity the Lethal Concentration （LC50）, Growth Inhibition （GI50） and Total Growth Inhibition （TGI） of the extract were investigated individually for each cancer celt line. Analysis of the extract has shown good cytotoxicity in all tested cancer cell lines, with an IC50 of 55 μg/mL against NCIH460 and U251, 65 μg/mL for HCT116 respectively. GI50 was found to be 5 μg/mL for NCIH 460 and 10 μg/mL for HCT 116 and U251 cell lines. TGI was 19 μg/mL for NCIH 460, 29 μg/mL for HCT 116 and 25 μg/mL for U 251 cell lines. The cytotoxicity of the extract were significantly high in Lung Cancer Cell line （NCIH 460） when compared to Colon Cancer Cell line （HCT 116） and Glial Cell Carcinoma （U251） in the following order NCIH 460 〉 HCT 116 〉 U251.展开更多
文摘The methanolic extract of red seaweed Kappaphycus alvarezii was evaluated against three different cancer cell lines to study for its antiproliferative effect. Lung cancer cell line （NCIH 460）, Colon cancer cell line （HCT 116） and Glial cell carcinoma （U 251） are the three selected cell lines investigated in this study. Different concentrations of methanol extract （0.01, 0.10, 1.00, 10.00 and 100.00 lag/mL respectively） of Kappaphycus alvarezii were prepared and screened by quantitative MTT （Microculture Tetrazolium） （3-（4, 5-dimethylthiazol）-2, 5-diphenyltetrazolium bromide） assay. MTT assay are the colorimetric assay which was applied to assess the viability and proliferation of cancer cells to determine the cytotoxicity of methanol extract ofK. alvarezii. The MTT test is based on the enzymatic reduction of the tetrazolium salt （MTT） to formazon crystals exclusively in living metabolically active cells developed purple color complex which was directly proportional to the viability of cells. To elucidate the in-vitro anticancer activity the Lethal Concentration （LC50）, Growth Inhibition （GI50） and Total Growth Inhibition （TGI） of the extract were investigated individually for each cancer celt line. Analysis of the extract has shown good cytotoxicity in all tested cancer cell lines, with an IC50 of 55 μg/mL against NCIH460 and U251, 65 μg/mL for HCT116 respectively. GI50 was found to be 5 μg/mL for NCIH 460 and 10 μg/mL for HCT 116 and U251 cell lines. TGI was 19 μg/mL for NCIH 460, 29 μg/mL for HCT 116 and 25 μg/mL for U 251 cell lines. The cytotoxicity of the extract were significantly high in Lung Cancer Cell line （NCIH 460） when compared to Colon Cancer Cell line （HCT 116） and Glial Cell Carcinoma （U251） in the following order NCIH 460 〉 HCT 116 〉 U251.
