Objective:The aim of this study is to investigate the phytochemical and the long-term anti-neuropathic potentials of Crocus sativus cultivated in the University botanical garden,and explore its most bioactive compound...Objective:The aim of this study is to investigate the phytochemical and the long-term anti-neuropathic potentials of Crocus sativus cultivated in the University botanical garden,and explore its most bioactive compounds and their underlying mechanisms of action.Methods:Phytochemical analysis and bio-guided isolation-procedures including RP-HPLC and1 H and13 C NMR utilizing biological models of diabetes,inflammation,and diabetic-neuropathy were used.Cultivated saffron(S-RCED)and Spanish-saffron stigma(S-SP)alone or in combination with Camellia sinus(CS)were investigated.Results:The RP-HPLC analyses showed the presence of picrocrocin,crocin I,crocin II,crocin I’,crocin II’,and safranal(SAF)in both S-SP and S-RCED extracts with higher-concentrations.It had been shown that SAF was the most bioactive-compound in Crocus sativus.Both S-SP and S-RCED possessed significant(P<0.05)anti-diabetic activities in acute(6 h),subchronic(8 d)and chronic(8 weeks)models.S-RCED had been proven with more hypoglycemic potentials when compared to S-SP and SAF.S-SP,S-RCED,and SAF produced significant anti-inflammatory and anti-nociceptive activities against carrageenan-induced inflammatory,hyperalgesic and tactile diabetic-neuropathy models,respectively.S-SP,S-RCED,and SAF elevated serum catalase,reduced glutathione,and insulin serum levels,ameliorated lipid peroxidation and Hb A1 c levels,and histopathologically regenerated the pancreatic beta-cells.Combinations with CS showed more significant efficacy than the single component.Conclusion:The oxidative stress reduction,insulin secretagogue,and pancreatic beta-cells regeneration potentials might be responsible for the mechanism underlying the anti-diabetic,anti-inflammatory and anti-diabetic neuropathy activities.Thus,the cultivated Crocus sativus might be clinically useful for protecting against many serious-disorders.展开更多
文摘Objective:The aim of this study is to investigate the phytochemical and the long-term anti-neuropathic potentials of Crocus sativus cultivated in the University botanical garden,and explore its most bioactive compounds and their underlying mechanisms of action.Methods:Phytochemical analysis and bio-guided isolation-procedures including RP-HPLC and1 H and13 C NMR utilizing biological models of diabetes,inflammation,and diabetic-neuropathy were used.Cultivated saffron(S-RCED)and Spanish-saffron stigma(S-SP)alone or in combination with Camellia sinus(CS)were investigated.Results:The RP-HPLC analyses showed the presence of picrocrocin,crocin I,crocin II,crocin I’,crocin II’,and safranal(SAF)in both S-SP and S-RCED extracts with higher-concentrations.It had been shown that SAF was the most bioactive-compound in Crocus sativus.Both S-SP and S-RCED possessed significant(P<0.05)anti-diabetic activities in acute(6 h),subchronic(8 d)and chronic(8 weeks)models.S-RCED had been proven with more hypoglycemic potentials when compared to S-SP and SAF.S-SP,S-RCED,and SAF produced significant anti-inflammatory and anti-nociceptive activities against carrageenan-induced inflammatory,hyperalgesic and tactile diabetic-neuropathy models,respectively.S-SP,S-RCED,and SAF elevated serum catalase,reduced glutathione,and insulin serum levels,ameliorated lipid peroxidation and Hb A1 c levels,and histopathologically regenerated the pancreatic beta-cells.Combinations with CS showed more significant efficacy than the single component.Conclusion:The oxidative stress reduction,insulin secretagogue,and pancreatic beta-cells regeneration potentials might be responsible for the mechanism underlying the anti-diabetic,anti-inflammatory and anti-diabetic neuropathy activities.Thus,the cultivated Crocus sativus might be clinically useful for protecting against many serious-disorders.
