Background: Cadmium is a heavy metal having toxic effects on most organ systems of the body. Objectives: We aim to investigate the postulated protective properties of olive oil in Cadmium-induced renal toxicity in mic...Background: Cadmium is a heavy metal having toxic effects on most organ systems of the body. Objectives: We aim to investigate the postulated protective properties of olive oil in Cadmium-induced renal toxicity in mice by utilizing standard biomarkers of renal toxicity, oxidative stress and histopathological characterization. Materials and Methods: Forty mice were randomly divided into four groups of 10 mice each. Group 1 served as control group, group 2 was given Extra-virgin olive oil orally, group 3 was given Cadmium chloride and group 4 was given Cadmium chloride with Extra-virgin olive oil. At the end of the experimental period, biochemical analysis histopathology of kidney was done. Results: The present study depicted that blood urea, serum creatinine and the antioxidant markers (Superoxide dismutase, Glutathione peroxidase and Catalase) levels were significantly increased in group 3 compared to group 1 and 2. After administration of Extra-virgin olive oil with Cadmium in group 4, the levels of those markers significantly improved. Histopathology of the renal tissue showed severe damage of glomeruli, severely congested blood vessels and marked dilatation of Bowman’s capsule in group 3 with improvements of these changes in group 4. Conclusion: This study suggests that Extra-virgin olive oil can be used as a cost effective safe anti-oxidative agent in the prevention of Cadmium toxicity.展开更多
Reaching a postmortem diagnosis of hypothermia is challenging in forensic practice.Therefore,this study was conducted to detect the histopathological,histochemical and biochemical changes that occur in adult albino ra...Reaching a postmortem diagnosis of hypothermia is challenging in forensic practice.Therefore,this study was conducted to detect the histopathological,histochemical and biochemical changes that occur in adult albino rats following exposure to induced fatal hypothermia.Twenty-four adult albino rats were divided into the negative control,moderate hypothermia,severe hypothermia and hypoxia groups.Rats in the control group were euthanized when those in the moderate hypothermic group died.Blood samples were collected via heart puncture,and the cerebrum,heart,suprarenal gland,kidney,liver and skeletal muscle were removed to investigate the biochemical,histochemical and histopathological changes.Postmortem assessment depicted significant changes in lipid peroxidation,represented by increased malondialdehyde levels in the studied organs of the rats in hypothermic and hypoxia groups.Histopathological examination of the rats’organs revealed degeneration and necrosis in the hypothermia and hypoxia groups.Sections taken from the severe hypothermic rats revealed a loss of normal cardiac tissue architecture,necrotic changes in the pyramidal cells in the cerebral cortex,and massive necrosis,mainly in the tubules of the renal cortex and medulla.These findings suggest that histological changes might be used as biochemical markers for postmortem diagnosing of fatal hypothermia,particularly in severe hypothermic conditions.展开更多
文摘Background: Cadmium is a heavy metal having toxic effects on most organ systems of the body. Objectives: We aim to investigate the postulated protective properties of olive oil in Cadmium-induced renal toxicity in mice by utilizing standard biomarkers of renal toxicity, oxidative stress and histopathological characterization. Materials and Methods: Forty mice were randomly divided into four groups of 10 mice each. Group 1 served as control group, group 2 was given Extra-virgin olive oil orally, group 3 was given Cadmium chloride and group 4 was given Cadmium chloride with Extra-virgin olive oil. At the end of the experimental period, biochemical analysis histopathology of kidney was done. Results: The present study depicted that blood urea, serum creatinine and the antioxidant markers (Superoxide dismutase, Glutathione peroxidase and Catalase) levels were significantly increased in group 3 compared to group 1 and 2. After administration of Extra-virgin olive oil with Cadmium in group 4, the levels of those markers significantly improved. Histopathology of the renal tissue showed severe damage of glomeruli, severely congested blood vessels and marked dilatation of Bowman’s capsule in group 3 with improvements of these changes in group 4. Conclusion: This study suggests that Extra-virgin olive oil can be used as a cost effective safe anti-oxidative agent in the prevention of Cadmium toxicity.
文摘Reaching a postmortem diagnosis of hypothermia is challenging in forensic practice.Therefore,this study was conducted to detect the histopathological,histochemical and biochemical changes that occur in adult albino rats following exposure to induced fatal hypothermia.Twenty-four adult albino rats were divided into the negative control,moderate hypothermia,severe hypothermia and hypoxia groups.Rats in the control group were euthanized when those in the moderate hypothermic group died.Blood samples were collected via heart puncture,and the cerebrum,heart,suprarenal gland,kidney,liver and skeletal muscle were removed to investigate the biochemical,histochemical and histopathological changes.Postmortem assessment depicted significant changes in lipid peroxidation,represented by increased malondialdehyde levels in the studied organs of the rats in hypothermic and hypoxia groups.Histopathological examination of the rats’organs revealed degeneration and necrosis in the hypothermia and hypoxia groups.Sections taken from the severe hypothermic rats revealed a loss of normal cardiac tissue architecture,necrotic changes in the pyramidal cells in the cerebral cortex,and massive necrosis,mainly in the tubules of the renal cortex and medulla.These findings suggest that histological changes might be used as biochemical markers for postmortem diagnosing of fatal hypothermia,particularly in severe hypothermic conditions.
