Solid phases of visible fuorescence substance(VFS)of biological fuids(blood,urine,hemodia-lysate)which was proposed earlier as a morbidity and mortality marker by renal failure and diabetes were investigated in-depth ...Solid phases of visible fuorescence substance(VFS)of biological fuids(blood,urine,hemodia-lysate)which was proposed earlier as a morbidity and mortality marker by renal failure and diabetes were investigated in-depth by the methods of electron and confocal microscopy,optical spectroscopy and matrix assisted laser desorption-ionization(MALDI)mass spectroscopy.It is shown that dry VFS exists predominantly in the form of carbon-oxygen-nitrogen(N≈8.7 wt.%)nanoparticles(NPs)(5≤d≤100nm).For the first time the existence of the threshold energy E_(g)≈2.15eV for excitation of VFS was observed experimentally and confirmed by semi empirical calculations of the bathochromic shift.A good accordance with the earlier autonomous theo-retical calculations was achieved.Thus,the long wavelength limit(575 nm)of the spectral range where the VFS can be used as a fluorescent marker was reliably determined.A pilot MALDI comparative study of graphene oxide(GO)and urine VFS was carried out.Six kinds of nitrogen-free particles(412≤M≤456 Da)were observed in each substance and possible computer models of those have been composed.It is established that along with nitrogen-containing advanced glycation end products(AGEs)also nitrogen-fee carbon-oxygen-lhydrogen particles(probably toxic)with the composition and structure related to GO can exist in biofuids.Both types of particles should be taken into account in search for the reasons of high mortality among end stage renal disease patients.展开更多
Background: Chronic kidney disease (CKD) is an irreversible decline in the glomerular filtration due to nephrosclerosis and glomerular loss. Rat remnant kidney model enables to assess the benefits of different treatme...Background: Chronic kidney disease (CKD) is an irreversible decline in the glomerular filtration due to nephrosclerosis and glomerular loss. Rat remnant kidney model enables to assess the benefits of different treatment possibilities. Aim: The aim of our study was to investigate renal morphology and functioning after 12 weeks of treatment with Equisetum arvense and Viscum album. Methods: Male Wistar rats with 5/6 nephrectomy received herbal drug preparation Equisetum/Viscum in a dosage of 0.007 g/kg/die (herbal group) or losartan (180 mg/l, ARB group) or remained untreated. Systolic blood pressure (SBP) and proteinuria were measured. Renal cortex tissue samples examined for focal-segmental glomerulosclerosis (FSGS) and interstitial fibrosis (IF). mRNA was isolated to estimate CCL2/MCP-1 gene transcription. Results: SBP was significantly lower both in herbal group and ARB group compared with untreated animals (p between ARB group and untreated NPX (p = 0.001). Quantitative RT-PCR analysis revealed statistically significant differences of mRNA transcription for MCP-1 between herbal group and untreated group (p Conclusions: We report about beneficial impact of horsetail Equisetum arvense and mistletoe Viscum album treatment on kidney functioning and morphology.展开更多
基金support for this work by the Archimedes project 3.2.1101.12-0011supported in part by the institutional research funding IUT (IUT 2-26)of the Estonian Ministry of Education and Research.
文摘Solid phases of visible fuorescence substance(VFS)of biological fuids(blood,urine,hemodia-lysate)which was proposed earlier as a morbidity and mortality marker by renal failure and diabetes were investigated in-depth by the methods of electron and confocal microscopy,optical spectroscopy and matrix assisted laser desorption-ionization(MALDI)mass spectroscopy.It is shown that dry VFS exists predominantly in the form of carbon-oxygen-nitrogen(N≈8.7 wt.%)nanoparticles(NPs)(5≤d≤100nm).For the first time the existence of the threshold energy E_(g)≈2.15eV for excitation of VFS was observed experimentally and confirmed by semi empirical calculations of the bathochromic shift.A good accordance with the earlier autonomous theo-retical calculations was achieved.Thus,the long wavelength limit(575 nm)of the spectral range where the VFS can be used as a fluorescent marker was reliably determined.A pilot MALDI comparative study of graphene oxide(GO)and urine VFS was carried out.Six kinds of nitrogen-free particles(412≤M≤456 Da)were observed in each substance and possible computer models of those have been composed.It is established that along with nitrogen-containing advanced glycation end products(AGEs)also nitrogen-fee carbon-oxygen-lhydrogen particles(probably toxic)with the composition and structure related to GO can exist in biofuids.Both types of particles should be taken into account in search for the reasons of high mortality among end stage renal disease patients.
文摘Background: Chronic kidney disease (CKD) is an irreversible decline in the glomerular filtration due to nephrosclerosis and glomerular loss. Rat remnant kidney model enables to assess the benefits of different treatment possibilities. Aim: The aim of our study was to investigate renal morphology and functioning after 12 weeks of treatment with Equisetum arvense and Viscum album. Methods: Male Wistar rats with 5/6 nephrectomy received herbal drug preparation Equisetum/Viscum in a dosage of 0.007 g/kg/die (herbal group) or losartan (180 mg/l, ARB group) or remained untreated. Systolic blood pressure (SBP) and proteinuria were measured. Renal cortex tissue samples examined for focal-segmental glomerulosclerosis (FSGS) and interstitial fibrosis (IF). mRNA was isolated to estimate CCL2/MCP-1 gene transcription. Results: SBP was significantly lower both in herbal group and ARB group compared with untreated animals (p between ARB group and untreated NPX (p = 0.001). Quantitative RT-PCR analysis revealed statistically significant differences of mRNA transcription for MCP-1 between herbal group and untreated group (p Conclusions: We report about beneficial impact of horsetail Equisetum arvense and mistletoe Viscum album treatment on kidney functioning and morphology.
