Objective:To study the effects of silent information regulator of transcription 2 (SIRT2) on inflammatory response and bone destruction in cartilage tissue of osteoarthritis.Methods: A total of 200 patients who underw...Objective:To study the effects of silent information regulator of transcription 2 (SIRT2) on inflammatory response and bone destruction in cartilage tissue of osteoarthritis.Methods: A total of 200 patients who underwent knee replacement due to knee osteoarthritis in Kashgar Prefecture First People's Hospital between September 2014 and September 2017 were selected as the osteoarthritis (OA) group of the research, and 80 patients who underwent knee replacement or meniscus operation due to trauma in Kashgar Prefecture First People's Hospital during the same period were selected as the control group. Articular cartilage tissue was collected after surgery to measure the expression of SIRT2 and bone destruction-related apoptosis molecules as well as the levels of inflammatory response molecules and bone destruction-related collagen metabolism molecules.Results: SIRT2 and Bcl-2 mRNA expression as well as SOX9 and Col-II levels in articular cartilage tissue of OA group were significantly lower than those of control group whereas TNF-α, bFGF, NO, IP-10, CCL2, PAR-2,β-catenin, OPN and MMP13 levels as well as Fas, GRP78, ATF6 and Caspase-3 mRNA expression were significantly higher than those of control group;SIRT2 mRNA expression in articular cartilage tissue of OA group was positively correlated with Bcl-2 mRNA expression as well as SOX9 and Col-II levels, and negatively correlated with TNF-α, bFGF, NO, IP-10, CCL2, PAR-2,β-catenin, OPN and MMP13 levels as well as Fas, GRP78, ATF6 and Caspase-3 mRNA expression.Conclusion: The lowly expressed SIRT2 in cartilage of osteoarthritis can aggravate inflammatory response and bone destruction.展开更多
文摘Objective:To study the effects of silent information regulator of transcription 2 (SIRT2) on inflammatory response and bone destruction in cartilage tissue of osteoarthritis.Methods: A total of 200 patients who underwent knee replacement due to knee osteoarthritis in Kashgar Prefecture First People's Hospital between September 2014 and September 2017 were selected as the osteoarthritis (OA) group of the research, and 80 patients who underwent knee replacement or meniscus operation due to trauma in Kashgar Prefecture First People's Hospital during the same period were selected as the control group. Articular cartilage tissue was collected after surgery to measure the expression of SIRT2 and bone destruction-related apoptosis molecules as well as the levels of inflammatory response molecules and bone destruction-related collagen metabolism molecules.Results: SIRT2 and Bcl-2 mRNA expression as well as SOX9 and Col-II levels in articular cartilage tissue of OA group were significantly lower than those of control group whereas TNF-α, bFGF, NO, IP-10, CCL2, PAR-2,β-catenin, OPN and MMP13 levels as well as Fas, GRP78, ATF6 and Caspase-3 mRNA expression were significantly higher than those of control group;SIRT2 mRNA expression in articular cartilage tissue of OA group was positively correlated with Bcl-2 mRNA expression as well as SOX9 and Col-II levels, and negatively correlated with TNF-α, bFGF, NO, IP-10, CCL2, PAR-2,β-catenin, OPN and MMP13 levels as well as Fas, GRP78, ATF6 and Caspase-3 mRNA expression.Conclusion: The lowly expressed SIRT2 in cartilage of osteoarthritis can aggravate inflammatory response and bone destruction.
