Objective: To evaluate the impact of Olea (O.) europaea extract on markers of oxidative stress and apoptosis of ovarian tissues in a rat model of torsion/detorsion-induced ovarian damage. Methods: A total of 28 Wistar...Objective: To evaluate the impact of Olea (O.) europaea extract on markers of oxidative stress and apoptosis of ovarian tissues in a rat model of torsion/detorsion-induced ovarian damage. Methods: A total of 28 Wistar female rats were randomly assigned into 4 groups, with 7 rats in each group. The sham group received a 2.5 cm longitudinal incision in the midline part of the abdomen which was then sutured with 5-0 nylon thread;the torsion/detorsion group underwent torsion induction for 3 h followed by reperfusion for 10 days;the torsion/detorsion+O. europaea group received 300 mg/kg hydro-alcoholic extract of O. europaea 30 min before detorsion, followed by reperfusion for 10 days;and the O. europaea group only received 300 mg/kg hydro-alcoholic extract of O. europaea for 10 days. After the treatment period, blood samples were taken;the levels of estrogen, glutathione peroxidase, superoxide dismutase, and malondialdehyde were assayed. The histological changes, as well as the rate of apoptosis in ovarian tissues, were also carried out by histomorphometric analysis at day 10 post-procedure. Results: Histological comparisons demonstrated a significant detrimental change in the torsion/detorsion group as compared with other groups. The number of pre-antral and antral follicles and corpus luteum was significantly decreased in the torsion/detorsion group compared with the sham group, while treatment with O. europaea could enhance their numbers (P<0.05). The index of apoptosis and the number of atretic body in the ovarian tissue were significantly higher in the torsion/detorsion group compared with the sham group (P<0.05). The concentrations of glutathione peroxidase, estrogen, and superoxide dismutase as well as the mRNA expression of Bcl-2 were considerably diminished in the torsion/detorsion group while they were elevated in the torsion/detorsion+O. europaea group (P<0.05) compared with the torsion/detorsion group. The serum malondialdehyde level and the mRNA expression of Bax were markedly increased during ischemia, while treatment with O. europaea significantly diminished the increased concentrations of malondialdehyde and Bax level in the torsion/detorsion+O. europaea group (P<0.05). Conclusions: O. europaea extract can reduce the degree of tissue damage induced by oxidative stress and apoptosis in the ovary following ovarian ischemia/reperfusion.展开更多
Background： The aim of this study was to design and assess the effects of hydroalcoholic extract of Matricaria chamomill^l （MC） on preantral follicle culture of mouse ovaries in a three-dimensional culture system. ...Background： The aim of this study was to design and assess the effects of hydroalcoholic extract of Matricaria chamomill^l （MC） on preantral follicle culture of mouse ovaries in a three-dimensional culture system. Methods： Isolated preantral follicles were randomly divided into three main groups： the control group containing 10% fetal bovine serum without MC extract （G1）, the first experimental group supplemented with 25 ktg/ml hydroalcoholic extract of chamomile （G2）, and the second experimental group supplemented with 50 p,g/ml hydroalcoholic extract of chamomile （G3）. Results： After 12 days of culture, the survival rate （P 〈 0.05）, antrum formation （P 〈 0.01）, metaphase two oocytes （P 〈 0.01）, and the expression ofPCNA （P 〈 0.05） and FSHR （P 〈 0.05） genes significantly decreased in G3 as compared with GI. On the other hand, at the last day of culture （day 12）, the mean diameter of follicles cultured in the medium which was supplemented with 50 lag/ml hydroalcoholic extract of chamomile significantly decreased as compared with the GI （P 〈 0.05）. In addition, the levels of progesterone and dehydroepiandrosterone hormones significantly increased in the medium of G3 relative to G I （P 〈 0.01）, while in the medium of G 1, the level of 17[3-estradiol was significantly higher than that of other groups （P 〈 0.01）. Reactive oxygen species levels of metaphase 11 oocytes were significantly decreased in G2 as compared with G1 （P 〈 0.01）. Conclusion： Adding chamomile extract to culture media appeared to decrease follicular function and development.展开更多
文摘Objective: To evaluate the impact of Olea (O.) europaea extract on markers of oxidative stress and apoptosis of ovarian tissues in a rat model of torsion/detorsion-induced ovarian damage. Methods: A total of 28 Wistar female rats were randomly assigned into 4 groups, with 7 rats in each group. The sham group received a 2.5 cm longitudinal incision in the midline part of the abdomen which was then sutured with 5-0 nylon thread;the torsion/detorsion group underwent torsion induction for 3 h followed by reperfusion for 10 days;the torsion/detorsion+O. europaea group received 300 mg/kg hydro-alcoholic extract of O. europaea 30 min before detorsion, followed by reperfusion for 10 days;and the O. europaea group only received 300 mg/kg hydro-alcoholic extract of O. europaea for 10 days. After the treatment period, blood samples were taken;the levels of estrogen, glutathione peroxidase, superoxide dismutase, and malondialdehyde were assayed. The histological changes, as well as the rate of apoptosis in ovarian tissues, were also carried out by histomorphometric analysis at day 10 post-procedure. Results: Histological comparisons demonstrated a significant detrimental change in the torsion/detorsion group as compared with other groups. The number of pre-antral and antral follicles and corpus luteum was significantly decreased in the torsion/detorsion group compared with the sham group, while treatment with O. europaea could enhance their numbers (P<0.05). The index of apoptosis and the number of atretic body in the ovarian tissue were significantly higher in the torsion/detorsion group compared with the sham group (P<0.05). The concentrations of glutathione peroxidase, estrogen, and superoxide dismutase as well as the mRNA expression of Bcl-2 were considerably diminished in the torsion/detorsion group while they were elevated in the torsion/detorsion+O. europaea group (P<0.05) compared with the torsion/detorsion group. The serum malondialdehyde level and the mRNA expression of Bax were markedly increased during ischemia, while treatment with O. europaea significantly diminished the increased concentrations of malondialdehyde and Bax level in the torsion/detorsion+O. europaea group (P<0.05). Conclusions: O. europaea extract can reduce the degree of tissue damage induced by oxidative stress and apoptosis in the ovary following ovarian ischemia/reperfusion.
文摘Background： The aim of this study was to design and assess the effects of hydroalcoholic extract of Matricaria chamomill^l （MC） on preantral follicle culture of mouse ovaries in a three-dimensional culture system. Methods： Isolated preantral follicles were randomly divided into three main groups： the control group containing 10% fetal bovine serum without MC extract （G1）, the first experimental group supplemented with 25 ktg/ml hydroalcoholic extract of chamomile （G2）, and the second experimental group supplemented with 50 p,g/ml hydroalcoholic extract of chamomile （G3）. Results： After 12 days of culture, the survival rate （P 〈 0.05）, antrum formation （P 〈 0.01）, metaphase two oocytes （P 〈 0.01）, and the expression ofPCNA （P 〈 0.05） and FSHR （P 〈 0.05） genes significantly decreased in G3 as compared with GI. On the other hand, at the last day of culture （day 12）, the mean diameter of follicles cultured in the medium which was supplemented with 50 lag/ml hydroalcoholic extract of chamomile significantly decreased as compared with the GI （P 〈 0.05）. In addition, the levels of progesterone and dehydroepiandrosterone hormones significantly increased in the medium of G3 relative to G I （P 〈 0.01）, while in the medium of G 1, the level of 17[3-estradiol was significantly higher than that of other groups （P 〈 0.01）. Reactive oxygen species levels of metaphase 11 oocytes were significantly decreased in G2 as compared with G1 （P 〈 0.01）. Conclusion： Adding chamomile extract to culture media appeared to decrease follicular function and development.
