Background: Respiratory distress syndrome (RDS) is a major cause of neonatal morbidity and mortality, affecting approximately 1% of all live births and 10% of all preterm infants. Lamellar bodies represent a storage f...Background: Respiratory distress syndrome (RDS) is a major cause of neonatal morbidity and mortality, affecting approximately 1% of all live births and 10% of all preterm infants. Lamellar bodies represent a storage form of pulmonary surfactant within Type II pneumocytes, secretion of which increases with advancing gestational age, thus enabling prediction of the degree of FLM. Preterm premature rupture of membranes (PPROM) complicates approximately 1/3 of all preterm births. Birth within 1 week is the most likely outcome for any patient with PPROM in the absence of adjunctive treatments. Respiratory distress has been reported to be the most common complication of preterm birth. Sepsis, intraventricular haemorrhage, and necrotizing enterocolitis also are associated with prematurity, but these are less common near to term. Objective: To assess the efficacy of the amniotic fluid lamellar body counting from a vaginal pool in predicting fetal lung maturity in women with preterm premature rupture of membranes. Methods: This study was conducted at Ain Shams University Maternity Hospital in the emergency ward from January 2019 to September 2019. It included 106 women with singleton pregnancies, gestational age from 28 - 36 weeks with preterm premature rupture of membranes. This study is designed to assess the efficacy of the amniotic fluid lamellar body counting (LBC) from a vaginal pool in predicting fetal lung maturity in women with preterm premature rupture of membranes. Results: The current study revealed a highly significant increase in the lamellar body count in cases giving birth to neonates without RDS compared to that cases giving birth to neonates with RDS. Also, no statistically significant difference between LBC and age, parity and number of previous miscarriages in the mother was found. Gestational age at delivery was significantly lower among cases with respiratory distress. Steroid administration was significantly less frequent among cases with respiratory distress. However, lamellar bodies had high diagnostic performance in the prediction of respiratory distress. Conclusion: Lamellar body count (LBC) is an effective, safe, easy, and cost-effective method to assess fetal lung maturity (FLM). It does not need a highly equipped laboratory or specially trained personnel, it just needs the conventional blood count analyzer. Measurement of LBC is now replacing the conventional Lecithin/Sphyngomyelin L/S ratio. LBC cut-off value of ≤42.5 × 10<sup>3</sup>/μL can be used safely to decide fetal lung maturity with sensitivity of 95.7% and specificity of 97.6%.展开更多
Assessment of oocyte quality to avoid overproduction of embryos is now considered an important goal in ICSI cycles. Although in classic Intra Cytoplasmic Sperm Injection (ICSI) cycles selection is mainly done for embr...Assessment of oocyte quality to avoid overproduction of embryos is now considered an important goal in ICSI cycles. Although in classic Intra Cytoplasmic Sperm Injection (ICSI) cycles selection is mainly done for embryos and not for follicles. Follicular fluid and its contents representing the oocyte environment are now gaining more attention because not only of its crucial influence on oocyte developments but also due to easy isolation of follicular fluid with every case of ICSI. By its aspiration during ovum pick up (OPU). substances isolated from follicular fluid to assess oocyte quality were in the form of: hormones like, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), Anti-mullerian Hormon (AMH), Growth Hormone (GH) etc., growth factors like Insulin Like Growth Factor (ILGF), proteins and amino acids like Lactoferrin (LF) which is an iron-binding glycoprotein that was detected in follicular fluid and was thought to be related to good oocyte quality when present in high concentration. Objectives: The aim of this study was to detect the possible effect of follicular fluid lactoferrin on oocyte quality and hence pregnancy rate in ICSI cycles. Methods: Follicular fluid was obtained from 64 patients undergoing Intra-cytoplasmic sperm injection (ICSI) procedure in Ain Shams University Maternity Hospital. Follicular fluid (FF) was collected at the time of oocyte harvesting. The lactoferrin concentration in FF was assayed by ELISA. Results: The mean LF concentration in follicular fluid of the positive biochemical pregnancy group (0.63 ± 0.17 ng/mL) was not significantly higher than that in the negative biochemical pregnancy (0.61 ± 0.16 ng/mL). A positive correlation between a number of mature oocytes and lactoferrin concentration was not found (r.101). Conclusion: Lactoferrin level in follicular fluid does not correlate with oocytes quality or pregnancy rate.展开更多
文摘Background: Respiratory distress syndrome (RDS) is a major cause of neonatal morbidity and mortality, affecting approximately 1% of all live births and 10% of all preterm infants. Lamellar bodies represent a storage form of pulmonary surfactant within Type II pneumocytes, secretion of which increases with advancing gestational age, thus enabling prediction of the degree of FLM. Preterm premature rupture of membranes (PPROM) complicates approximately 1/3 of all preterm births. Birth within 1 week is the most likely outcome for any patient with PPROM in the absence of adjunctive treatments. Respiratory distress has been reported to be the most common complication of preterm birth. Sepsis, intraventricular haemorrhage, and necrotizing enterocolitis also are associated with prematurity, but these are less common near to term. Objective: To assess the efficacy of the amniotic fluid lamellar body counting from a vaginal pool in predicting fetal lung maturity in women with preterm premature rupture of membranes. Methods: This study was conducted at Ain Shams University Maternity Hospital in the emergency ward from January 2019 to September 2019. It included 106 women with singleton pregnancies, gestational age from 28 - 36 weeks with preterm premature rupture of membranes. This study is designed to assess the efficacy of the amniotic fluid lamellar body counting (LBC) from a vaginal pool in predicting fetal lung maturity in women with preterm premature rupture of membranes. Results: The current study revealed a highly significant increase in the lamellar body count in cases giving birth to neonates without RDS compared to that cases giving birth to neonates with RDS. Also, no statistically significant difference between LBC and age, parity and number of previous miscarriages in the mother was found. Gestational age at delivery was significantly lower among cases with respiratory distress. Steroid administration was significantly less frequent among cases with respiratory distress. However, lamellar bodies had high diagnostic performance in the prediction of respiratory distress. Conclusion: Lamellar body count (LBC) is an effective, safe, easy, and cost-effective method to assess fetal lung maturity (FLM). It does not need a highly equipped laboratory or specially trained personnel, it just needs the conventional blood count analyzer. Measurement of LBC is now replacing the conventional Lecithin/Sphyngomyelin L/S ratio. LBC cut-off value of ≤42.5 × 10<sup>3</sup>/μL can be used safely to decide fetal lung maturity with sensitivity of 95.7% and specificity of 97.6%.
文摘Assessment of oocyte quality to avoid overproduction of embryos is now considered an important goal in ICSI cycles. Although in classic Intra Cytoplasmic Sperm Injection (ICSI) cycles selection is mainly done for embryos and not for follicles. Follicular fluid and its contents representing the oocyte environment are now gaining more attention because not only of its crucial influence on oocyte developments but also due to easy isolation of follicular fluid with every case of ICSI. By its aspiration during ovum pick up (OPU). substances isolated from follicular fluid to assess oocyte quality were in the form of: hormones like, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), Anti-mullerian Hormon (AMH), Growth Hormone (GH) etc., growth factors like Insulin Like Growth Factor (ILGF), proteins and amino acids like Lactoferrin (LF) which is an iron-binding glycoprotein that was detected in follicular fluid and was thought to be related to good oocyte quality when present in high concentration. Objectives: The aim of this study was to detect the possible effect of follicular fluid lactoferrin on oocyte quality and hence pregnancy rate in ICSI cycles. Methods: Follicular fluid was obtained from 64 patients undergoing Intra-cytoplasmic sperm injection (ICSI) procedure in Ain Shams University Maternity Hospital. Follicular fluid (FF) was collected at the time of oocyte harvesting. The lactoferrin concentration in FF was assayed by ELISA. Results: The mean LF concentration in follicular fluid of the positive biochemical pregnancy group (0.63 ± 0.17 ng/mL) was not significantly higher than that in the negative biochemical pregnancy (0.61 ± 0.16 ng/mL). A positive correlation between a number of mature oocytes and lactoferrin concentration was not found (r.101). Conclusion: Lactoferrin level in follicular fluid does not correlate with oocytes quality or pregnancy rate.
