A 1500 bp DNA fragment of LsNPV genome was sequenced by silver sequencing system.A whole coding region of ORF1 and an incomplete ORF2 were found.ORF1 was 345 bp long and was predicted to encode a protein with 115 amin...A 1500 bp DNA fragment of LsNPV genome was sequenced by silver sequencing system.A whole coding region of ORF1 and an incomplete ORF2 were found.ORF1 was 345 bp long and was predicted to encode a protein with 115 amino acid residues,the 5'regulatory region of ORF1 contained early transcriptional consensus sequence such as AT/ACGTGT,CGTGC and a stem loop structure.On the 3'terminus of ORF1,a poly(A)tail signal was found.Incomplete ORF2 was 354 bp long and predicted to encode 118 amino acid residues,the 5'regulatory region of ORF2 contained two conserved late transcriptional motif ATAAG.Compared with all the genes from AcNPV,incomplete ORF2 showed 56.5%nucleotide sequence homology and 44.9%amino acid sequence homology with AcNPV PDV E66 gene.Two conserved ATAAG motif on 5'regulatory region of ORF2 was similar to those in AcNPV PDV E66 gene.ORF1 showed no high identity with AcNPV and other baculoviruses.展开更多
文摘A 1500 bp DNA fragment of LsNPV genome was sequenced by silver sequencing system.A whole coding region of ORF1 and an incomplete ORF2 were found.ORF1 was 345 bp long and was predicted to encode a protein with 115 amino acid residues,the 5'regulatory region of ORF1 contained early transcriptional consensus sequence such as AT/ACGTGT,CGTGC and a stem loop structure.On the 3'terminus of ORF1,a poly(A)tail signal was found.Incomplete ORF2 was 354 bp long and predicted to encode 118 amino acid residues,the 5'regulatory region of ORF2 contained two conserved late transcriptional motif ATAAG.Compared with all the genes from AcNPV,incomplete ORF2 showed 56.5%nucleotide sequence homology and 44.9%amino acid sequence homology with AcNPV PDV E66 gene.Two conserved ATAAG motif on 5'regulatory region of ORF2 was similar to those in AcNPV PDV E66 gene.ORF1 showed no high identity with AcNPV and other baculoviruses.
