Epigenetic modifications have been proved to be a powerful way to activate silent gene clusters and lead to diverse secondary metabolites in fungi. Previously, inactivation of a histone H3 deacetylase in Calcarisporiu...Epigenetic modifications have been proved to be a powerful way to activate silent gene clusters and lead to diverse secondary metabolites in fungi. Previously, inactivation of a histone H3 deacetylase in Calcarisporium arbuscula had led to pleiotropic activation and overexpression of more than 75% of the biosynthetic genes and isolation of ten compounds. Further investigation of the crude extract of C. arbuscula Δhda A strain resulted in the isolation of twelve new diterpenoids including three cassanes(1-3), one cleistanthane(4), six pimaranes(5-10), and two isopimaranes(11 and 12) along with two know cleistanthane analogues. Their structures were elucidated by extensive NMR spectroscopic data analysis. Compounds 2 and 4 showed potent inhibitory effects on the expression of MMP1 and MMP2(matrix metalloproteinases family) in human breast cancer(MCF-7) cells.展开更多
Objective To study the chemical constituents of the fungus Ganoderma cochlear. Methods The compounds were isolated by using MCI gel CHP 20P, Sephadex LH-20, RP-18 column chromatography, and preparative TLC. The struct...Objective To study the chemical constituents of the fungus Ganoderma cochlear. Methods The compounds were isolated by using MCI gel CHP 20P, Sephadex LH-20, RP-18 column chromatography, and preparative TLC. The structures were identified by means of spectroscopic methods. Results Two phenolic normeroterpenoid and meroterpenoid, cochlearols C and D （1 and 2）, together with six benzene derivatives, 3- methoxy-4-hydroxy-phenylethanol （3）, 4-hydroxyacetophenone （4）, p-hydroxycinnamic methyl ester （5）, 2-methoxy-4-hydroxybenzaldehyde （6）, 4-hydroxy-3-methoxy benzoic acid （7）, and 2-hydroxy-5-ethoxybenzoic acid （8）, were isolated from the fruiting bodies of Ganoderma cochlear. Conclusion Compounds 1 and 2 are new phenolic normeroterpenoid and meroterpenoid, respectively.展开更多
基金supported financially by National Natural Science Foundation of China (Nos. 21502233 and 81522043)CAMS Initiative for Innovative Medicine (CAMS-I2M-1-010)+1 种基金the PUMC Youth Fund (33320140175)the State Key Laboratory Fund for Excellent Young Scientists to Youcai Hu (GTZB201401)
文摘Epigenetic modifications have been proved to be a powerful way to activate silent gene clusters and lead to diverse secondary metabolites in fungi. Previously, inactivation of a histone H3 deacetylase in Calcarisporium arbuscula had led to pleiotropic activation and overexpression of more than 75% of the biosynthetic genes and isolation of ten compounds. Further investigation of the crude extract of C. arbuscula Δhda A strain resulted in the isolation of twelve new diterpenoids including three cassanes(1-3), one cleistanthane(4), six pimaranes(5-10), and two isopimaranes(11 and 12) along with two know cleistanthane analogues. Their structures were elucidated by extensive NMR spectroscopic data analysis. Compounds 2 and 4 showed potent inhibitory effects on the expression of MMP1 and MMP2(matrix metalloproteinases family) in human breast cancer(MCF-7) cells.
基金NSFC-Joint Foundation of Yunnan Province(U1202222)National Natural Science Foundation of China(21472199)+1 种基金a project from State Key Laboratory of Phytochemistry and Plant Resources in West ChinaKunming Institute of Botany(P2013-ZZ03)
文摘Objective To study the chemical constituents of the fungus Ganoderma cochlear. Methods The compounds were isolated by using MCI gel CHP 20P, Sephadex LH-20, RP-18 column chromatography, and preparative TLC. The structures were identified by means of spectroscopic methods. Results Two phenolic normeroterpenoid and meroterpenoid, cochlearols C and D （1 and 2）, together with six benzene derivatives, 3- methoxy-4-hydroxy-phenylethanol （3）, 4-hydroxyacetophenone （4）, p-hydroxycinnamic methyl ester （5）, 2-methoxy-4-hydroxybenzaldehyde （6）, 4-hydroxy-3-methoxy benzoic acid （7）, and 2-hydroxy-5-ethoxybenzoic acid （8）, were isolated from the fruiting bodies of Ganoderma cochlear. Conclusion Compounds 1 and 2 are new phenolic normeroterpenoid and meroterpenoid, respectively.
