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宏基因组二代测序在实体器官移植感染防控中的应用 被引量:1
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作者 满霖 李小杉 +5 位作者 王文静 钱婷 熊敏 杨航 陈静瑜 吴波 《器官移植》 CAS CSCD 北大核心 2024年第2期289-296,共8页
器官移植已成为多种终末期疾病的有效治疗手段,但器官移植受者术后需长期服用免疫抑制药,导致免疫功能低下,使得细菌、病毒和真菌感染的发生率相对较高。以病原学培养、免疫学检测和聚合酶链反应为代表的传统微生物检测方法被广泛用于... 器官移植已成为多种终末期疾病的有效治疗手段,但器官移植受者术后需长期服用免疫抑制药,导致免疫功能低下,使得细菌、病毒和真菌感染的发生率相对较高。以病原学培养、免疫学检测和聚合酶链反应为代表的传统微生物检测方法被广泛用于感染检测,但存在耗时长、需预先假定病原体等问题。宏基因组二代测序由于具有病原体检出率高、对病原谱检测全面的优点,近年来被广泛应用于器官移植领域的感染防控。本文就宏基因组二代测序技术在实体器官移植感染防控上的应用现状进行综述,以期对移植相关感染的诊断和治疗提供参考。 展开更多
关键词 器官移植 宏基因组二代测序 免疫抑制 肺部感染 供者来源性感染 感染防控 病原学检测 聚合酶链反应(PCR)
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肺移植术后肺部细菌感染的危险因素及其对死亡风险的影响分析 被引量:3
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作者 钱婷 李小杉 +5 位作者 王文静 满霖 范立 杨航 陈静瑜 吴波 《东南大学学报(医学版)》 CAS 2023年第3期348-356,共9页
目的:探讨肺移植术后肺部细菌感染的危险因素及其对死亡风险的影响。方法:回顾性分析南京医科大学附属无锡人民医院2019年1月至2020年12月期间行肺移植手术的274例患者的临床资料,采用单因素及多因素二分类非条件Logistic回归探索肺部... 目的:探讨肺移植术后肺部细菌感染的危险因素及其对死亡风险的影响。方法:回顾性分析南京医科大学附属无锡人民医院2019年1月至2020年12月期间行肺移植手术的274例患者的临床资料,采用单因素及多因素二分类非条件Logistic回归探索肺部细菌感染的影响因素,通过Kaplan-Meier法和Cox回归分析肺部细菌感染对死亡风险的影响。结果:供肺细菌感染(OR=2.859,95%CI 1.202~6.801)和受者术前肺部细菌感染(OR=2.812,95%CI 1.043~7.576)是术后肺部细菌感染的潜在危险因素。术后肺部细菌感染者短期死亡风险较未感染者低,但随着时间的推移,其死亡风险逐渐增加,而未感染者趋于平稳(HR=1.628,95%CI 0.816~3.249)。结论:供肺细菌感染和受者术前肺部细菌感染是肺移植术后早期肺部细菌感染的危险因素,但尚未观察到术后早期肺部细菌感染对1年死亡风险具有显著影响。 展开更多
关键词 肺移植 肺部细菌感染 危险因素 死亡风险
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腹腔镜胃袖状切除术对肥胖型多囊卵巢综合征代谢的影响(附33例报告) 被引量:4
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作者 马驰野 朱江帆 +4 位作者 马颖璋 满琳 陆伟 张启颖 张亚丽 《外科理论与实践》 2018年第6期506-510,共5页
目的 :探讨腹腔镜胃袖状切除术(laparoscopic sleeve gastrectomy, LSG)对肥胖型多囊卵巢综合征(polycystic ovary syndrome, PCOS)代谢改善的临床效果。方法:回顾分析我院33例接受LSG的肥胖型PCOS病例临床资料和术后6个月内随访结果。... 目的 :探讨腹腔镜胃袖状切除术(laparoscopic sleeve gastrectomy, LSG)对肥胖型多囊卵巢综合征(polycystic ovary syndrome, PCOS)代谢改善的临床效果。方法:回顾分析我院33例接受LSG的肥胖型PCOS病例临床资料和术后6个月内随访结果。术后3、6个月复查代谢指标变化。结果:本研究PCOS病人平均年龄(27±5)岁,体质量指数(body mass index, BMI)均≥27.5,均伴月经紊乱或停经,按共识确诊为PCOS。术前BMI为(36.75±4.96),血清睾酮为(0.489±0.186)μg/L,促黄体素/促卵泡素(LH/FSH)为(1.72±0.90),胰岛素抵抗指数(HOMA-IR)为(14.14±16.79),糖化血红蛋白(HbA1c)为6.35%±1.41%。33例均顺利完成手术,无中转开腹和严重并发症发生。病人术后3、6个月BMI分别为(29.55±4.65)和(25.07±3.81),睾酮分别为(0.336±0.112)μg/L和(0.216±0.115)μg/L,LH/FSH分别为(1.68±1.08)和(1.28±0.95),HOMA-IR分别为(3.07±2.14)和(2.35±1.14),HbA1c分别为5.35%±0.40%和5.22%±0.30%。除LH/FSH(P>0.05)外,其他代谢指标术后3、6个月与术前差异均有统计学意义(P<0.05),且随时间延长呈下降趋势。术后3、6个月分别有11例(33.3%)和26(78.8%)月经紊乱情况得到缓解。术后3、6个月PCOS临床缓解率分别为51.5%(17例)和78.8%(26例)。结论:LSG可改善肥胖型PCOS病例的月经和排卵,缓解高雄激素血症等PCOS症状,其机制可能与胰岛素抵抗的减轻密切相关。 展开更多
关键词 胃袖状切除术 多囊卵巢综合征 肥胖症 高雄激素血症 胰岛素抵抗
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An iterative computation method for interpreting and extending an analytical battery model
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作者 Neng-gan ZHENG Zhao-hui WU +1 位作者 man lin Qi-jia WANG 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2008年第2期279-288,共10页
Battery models are of great importance to develop portable computing systems,for whether the design of low power hardware architecture or the design of battery-aware scheduling policies.In this paper,we present a phys... Battery models are of great importance to develop portable computing systems,for whether the design of low power hardware architecture or the design of battery-aware scheduling policies.In this paper,we present a physically justified iterative computing method to illustrate the discharge,recovery and charge process of Li/Li-ion batteries.The discharge and recovery processes correspond well to an existing accurate analytical battery model:R-V-W's analytical model,and thus interpret this model algorithmically.Our method can also extend R-V-W's model easily to accommodate the charge process.The work will help the system designers to grasp the characteristics of R-V-W's battery model and also,enable to predict the battery behavior in the charge process in a uniform way as the discharge process and the recovery process.Experiments are performed to show the ac-curacy of the extended model by comparing the predicted charge times with those derived from the DUALFOIL simulations.Various profiles with different combinations of battery modes were tested.The experimental results show that the extended battery model preserves high accuracy in predicting the charge behavior. 展开更多
关键词 Analytical battery model lterative computation method Capacity response Charge DISCHARGE
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Prevalence and Risk Factors Associated with Prehypertension among Young and Middle-Aged Health Check-Up Population in Guangzhou
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作者 Lulu Yan Xia Lu +4 位作者 Yao Zhong man lin Fangfang Lu Rui Wang Tianhui You 《Journal of Advances in Medicine Science》 2021年第1期1-11,共11页
Objective:To provide basic information and theories for prehypertension early intervention,a systematic analysis of the epidemic status and risk factors among young and middle-aged was carried out here.Methods:This st... Objective:To provide basic information and theories for prehypertension early intervention,a systematic analysis of the epidemic status and risk factors among young and middle-aged was carried out here.Methods:This study relied on the data bank of a health check-up population of a class a tertiary general hospital in Guangdong province in 2015.Total 9540 young and middle-aged adults were enrolled,and 733 people were included to find out the effect with lifestyle in these crowd.Principal Components Analysis(PCA)of Factor(FA)was used to identify dietary patterns.The logistic regression model was used to find the risk factors of prehypertension.Results:Among 9540 young and middle-aged cases,the incidence of prehypertension was 36.6%.Moreover,the average age,proportion of male gender,overweight,FBG(fasting blood glucose),dyslipidemia,and hyperuricemia were significantly higher in the prehypertension group than in the optimal BP group.Multivariate logistic regression analysis indicated that age,total cholesterol,triglycerides,uric acid,body mass index and HR(heart rate)were risk factors,and female was a protective factor for prehypertension.Among 733 cases,the incidence of prehypertension was 35.1%.The proportion of smoking,drinking,physical workers,moderate and severe physical activity,and the intake of meat,dietary energy were significantly higher in the prehypertension group than in the optimal BP group.Dietary patterns included“meat model”,“spice model”,“main vegeTables model”and“high protein model”.Multivariate logistic regression analysis indicated that age,drinking were risk factors for prehypertension,while dietary milk intake,dietary magnesium intake were protective factors.Conclusions:Prehypertension is highly prevalent in Guangzhou.However,education about effective lifestyle modifications as an alcohol limit,increasing the intake of dairy products,and magnesium may intervene in the development of prehypertension.But how to develop targeted interventions for such groups need to be further explored.The present study would lay the theoretical foundation and basic data for the next step. 展开更多
关键词 PREHYPERTENSION Health examination Healthy lifestyle characteristics Dietary patterns NUTRITION Risk factors
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Ladder-Type Thienoacenaphthopyrazine-Based Molecules:Synthesis,Properties,and Application to Construct High-Performance Polymer for Organic Solar Cells
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作者 Pengwei Han man lin +5 位作者 Qiuju Jiang Haijun Ning Mingbin Su Li Dang Feng He Qinghe Wu 《CCS Chemistry》 CSCD 2023年第6期1318-1331,共14页
We present a new design strategy to synthesize ladder-type thienoacenaphthopyrazine(TAP)-based monomers through two different coupling reactions from starting material 5,6-dibromoacenaphthylene-1,2-dione.By bringing v... We present a new design strategy to synthesize ladder-type thienoacenaphthopyrazine(TAP)-based monomers through two different coupling reactions from starting material 5,6-dibromoacenaphthylene-1,2-dione.By bringing varous electron-deficient groups together(such as five-membered aromatic rings,pyrazine,fluorine,and thiadiazols),this new family of molecules exhibits good stability in ambient conditions and easily tunes the electronic,photophysical and film-forming properties of polymers.The unique molecular shape with its extendedπ-conjugated backbone perpendicular to the polymer chain,offers a remarkable platform for the development of a semiconducting polymer with rare geometry.The planar and enlarged conjugated backbone enables TAP-based copolymers PTAP1 and PTAP2 to exhibit distinguishing solubility properties that are different from small-molecule-based polymers,indicating the advantages of novel ladder-type monomers.Their insolubility in chloroform supports orthogonal solvent processing to fabricate layer-by-layer organic solar cells(LBL-OSCs).Owing to its shorterπ–πstacking distance,higher hole mobility,and more favorable phase separation caused bymultifluorine substitution,polymer PTAP1-based LBL-OSC exhibits the highest efficiency of 17.14%by using Y6-BO as the electron acceptor while the efficiency for the PTAP2-based device is 14.20%.This high efficiency indicates the tremendous potential of these novel building units. 展开更多
关键词 ladder-type monomer thienoacenaphthopyrazine polymer donor organic solar cell
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动脉性肺动脉高压特异性差异表达基因的筛选及生物信息学分析
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作者 钱婷 李小杉 +5 位作者 王文静 满霖 范立 杨航 陈静瑜 吴波 《现代医学》 2023年第12期1697-1704,共8页
目的:筛选动脉性肺动脉高压(PAH)相关的特异性差异表达基因,了解相关生物学功能,为进一步寻找PAH潜在调控靶点提供理论依据。方法:从美国国立生物技术信息中心的基因表达数据库(GEO)下载GSE117261和GSE113439高通量基因表达数据集。筛查... 目的:筛选动脉性肺动脉高压(PAH)相关的特异性差异表达基因,了解相关生物学功能,为进一步寻找PAH潜在调控靶点提供理论依据。方法:从美国国立生物技术信息中心的基因表达数据库(GEO)下载GSE117261和GSE113439高通量基因表达数据集。筛查PAH患者和对照人群的差异表达基因。利用“WGCNA”包进行加权基因共表达网络分析(WGCNA)以鉴定PAH表型特异性基因。将两者重叠部分定义为PAH特异性差异表达基因。并通过基因本体(gene ontology, GO)及京都基因和基因组百科全书(KEGG)分析进一步探索其潜在的生物学功能。此外,通过构建蛋白质-蛋白质交互作用(PPI)网络,以识别枢纽基因。结果:共获得414个差异表达基因,其中230个基因显著上调,184个基因显著下调。MEmidnightblue模块中919个基因为PAH表型特异性基因。确定了其交集的135个基因为PAH特异性差异表达基因。GO和KEGG分析表明,差异表达基因主要涉及细胞增殖、细胞趋化和炎症反应等生物学过程。基于PPI网络,将POSTN、ILR2、ILR4、CD163、KIT鉴定为枢纽基因。结论:POSTN、ILR2、ILR4、CD163、KIT可能是参与PAH发生的特异性差异表达基因,这为探寻潜在生物标志物和药物靶点提供了理论基础。 展开更多
关键词 动脉性肺动脉高压 特异性差异表达基因 细胞增殖 细胞趋化 炎症反应
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A Township with 8,000 Weiqi Players
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作者 man lin 《Women of China》 1996年第10期40-42,共3页
TANGQIAO is a township of Zhangjiagang City, Jiangsu Province. Ordinary in other ways, this town is unusual for the number of weiqi (go) players it boasts—8,000 of its population of 28,000. Almost every family has mo... TANGQIAO is a township of Zhangjiagang City, Jiangsu Province. Ordinary in other ways, this town is unusual for the number of weiqi (go) players it boasts—8,000 of its population of 28,000. Almost every family has more than one weiqi enthusiast. In recent years, the township’s players have earned themselves uncountable championships and runner-up placements at provincial-and national-level weiqi competitions. Thus, the State Physical Culture and Sports Commission awarded Zhangjiagang City the title "National Home of Weiqi" in July, 1990. In May of 1991, the Jiangsu Physical Culture 展开更多
关键词 A Township with 8 000 Weiqi Players
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